LOCUS       sequence-207554-        1800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR 
            intergenic region. The Landing Pad consists of floxed mCherry,Chlor 
            that are cotranscribed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Urtecho G, Tripp AD, Insigne K, Kim H, Kosuri S
  TITLE     Systematic Dissection of Sequence Elements Controlling sigma70 
            Promoters Using a Genomically-Encoded Multiplexed Reporter Assay in 
            E. coli.
  JOURNAL   Biochemistry. 2018 Feb 1. doi: 10.1021/acs.biochem.7b01069.
  PUBMED    29388765
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Feb 1. doi: 10.1021/acs.biochem.7b01069."
COMMENT     Sequence Label: E. coli K-12 MG1655_rLP5(nth-ydgR)
FEATURES             Location/Qualifiers
     source          1..1800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(123..782)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     697..716
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    1697..1730
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctgctttccg ctcaggcgac cgatgtcagt gttaataagg cgacggcgaa gcaagcggta
       61 cactcagttc taccgttcgt ataagaaacc atatacgaag ttatattacc ctgttatccc
      121 tattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
      181 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat caacaccttg
      241 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
      301 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata
      361 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
      421 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
      481 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
      541 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
      601 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
      661 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
      721 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
      781 atctagtaat ccgccttata tttgatatat gagattctag tattatttgt acagctcatc
      841 catgcccttg gaaaccatct agtaatccgc cttatatttg atatatgaga ttctagtatt
      901 atttgtacag ctcatccatg ccaccggtag aatgacgacc ctccgcgcgc tcatattgct
      961 ctacgatcgt ataatcttca ttatgagagg tgatgtccag tttaatattc acattgtacg
     1021 cgccaggcag ctgcacaggt ttcttggctt tgtacgtggt tttcacttca gcgtcataat
     1081 ggccgccatc tttcagtttc aggcgctgtt taatttcgcc tttcagcgca ccatcttccg
     1141 gatacatgcg ttcgctagac gcctcccaac ccatcgtctt tttctgcatc accgggccat
     1201 cagatggaaa attagtacca cgcagtttaa ctttatagat gaactcgcca tcctgcaggg
     1261 aggagtcctg agtgacggtc acgacaccac catcttcaaa attcattacg cgttcccact
     1321 tgaaaccttc cggaaaagac agcttcagat agtccgggat atccgctggg tgtttaacat
     1381 acgctttaga accgtacata aattgcgggc tcaggatgtc ccacgcaaaa ggcagcgggc
     1441 cgcctttagt cactttcagt ttggcggtct gggtgccttc atacggacgg ccctcgcctt
     1501 cgccttcgat ctcgaactcg tgaccgttaa cagaaccctc catgtgaact ttgaagcgca
     1561 tgaactcttt aatgatagcc atgttatcct cctcgccctt ggaaaccatc tagtaatcca
     1621 ccctatattt gatatatgag attctagtaa tgaaataatt atgcagaaaa attttcctga
     1681 agtcaatact ctttttataa cttcgtataa tgtatgctat acgaacggta agttctagga
     1741 cggacgtgtt aacactgcat tcggctggcc gactattgct gtcgacacgc acattttccg
//
