LOCUS       sequence-207556-        8124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin 
            resistance cassette downstream used to replace the native copy of 
            Vipp1 in Synechocystis (sll0617)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Gutu A, Chang F, O'Shea EK
  TITLE     Dynamical localization of a thylakoid membrane binding protein is 
            required for acquisition of photosynthetic competency.
  JOURNAL   Mol Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912.
  PUBMED    29357135
REFERENCE   2  (bases 1 to 8124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912."
COMMENT     Sequence Label: pAGH42
FEATURES             Location/Qualifiers
     source          1..8124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(2546..3337)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(5012..5030)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5013..5030)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5048..5064)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5048..5064)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5061..5083)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5072..5088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5096..5102,5103..5120,5121..5126))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5141..5162
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5279..5296)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5450..6038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5530..5549)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6209..7000,7001..7069))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6832..6851
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7070..7174)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7242..7260
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7298..7320)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7420..7439
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7506..7961)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7643..7664)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7855..7874
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8087..8109
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8101..8118
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8102..8118
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattcgag ctcggtaccc gggtaacgaa aggattcggc
       61 gggtagggca aaatcttgtc agcagactag gaatagcaat taagcccaag cccaactact
      121 ttaactgttc aagttgtttt tccctcattt cactgggctt tttaatattt ggctttggag
      181 ccatccccaa taatttttga ttaatttgtt ccctggtgtt tttgctataa ctagcccagg
      241 gatcgggcaa aaacatatca ccgatgctga taaaagccag accagcaatc accgctggaa
      301 tggcaaggga gcgcaaaatt ttaaatagac ttgccataac gctatgcctc cacaatcaaa
      361 tctcgattgg actacctgtg tttccattct agggtagggc accatggcga aagccactaa
      421 aaccaactcc aaggctgtct agtgtctgcg ggcttggttt acactggatc aacatagggc
      481 caactcctta gtccccctta tgcggacttc tgtacagacc cccattcgtt aacccccagc
      541 gattttgaga ggataagtaa gacatgggat tatttgaccg tttaggccgc gtcgtccggg
      601 ctaacctcaa cgacctcgtc agtaaagctg aagatccaga aaaagttctg gaacaggccg
      661 tgatcgatat gcaggaagac ctggtgcaac tccgccaggc cgttgcccgc acgatcgccg
      721 aagaaaaacg aacagagcaa cgtcttaacc aagacaccca ggaagccaag aaatgggaag
      781 accgggcaaa attagccctc accaatgggg aagaaaattt ggctagggaa gccctggccc
      841 gcaaaaaaag tctgacagat acggcggcgg cgtaccaaac ccagctagcc caacagagga
      901 ccatgtcgga gaatctccgt cgcaacctcg cggctctaga agcaaaaatt tccgaagcta
      961 aaaccaagaa aaatatgttg caggccaggg ccaaagcggc caaggctaat gctgaactgc
     1021 agcaaaccct cgggggctta ggtactagca gtgctactag tgcttttgaa cggatggaga
     1081 acaaggtact ggatatggaa gccacttccc aagcggcagg ggagttagcc ggctttggca
     1141 tcgagaacca gtttgcccag ttggaagcca gcagtggggt agaagacgag ttggccgccc
     1201 tgaaagcttc catggccggt ggagcattac cgggaacctc tgccgctacg ccccaactcg
     1261 aagcggctcc tgttgattcc tcagtaccag ctaataatgc cagtcaagat gatgcggtga
     1321 ttgaccagga attggacgac ctacgtcgtc ggttaaataa tctgagtggt ggtggaggta
     1381 gcaaaggcga ggagctcttt actggcgttg tccccattct cgtcgaacta gacggggatg
     1441 ttaatggcca taagttctcc gtgagtggcg aaggggaagg tgatgccacg tatggcaaac
     1501 tgacgctgaa atttatctgt accaccggga aattgccggt tccttggcca acactcgtta
     1561 ccacctttgg ctatggcgtc caatgctttg cccgttaccc cgatcacatg aagcagcatg
     1621 acttcttcaa aagcgccatg ccagagggtt atgtccaaga acgcacgatc ttcttcaagg
     1681 acgatggcaa ctacaagacc cgagctgaag tgaagtttga gggagatacg ttggtgaatc
     1741 gcatcgaact gaaagggatc gactttaaag aggatggcaa tattctgggg cataaactgg
     1801 agtacaacta caattcgcat aacgtgtaca ttatggcgga taagcagaag aatggcatca
     1861 aggtcaactt caagattcgg cacaatatcg aagatggttc agtgcaacta gcggaccact
     1921 atcagcagaa cacaccgatt ggcgatggtc ctgtcctgtt gccggataac cactatctga
     1981 gcactcaatc gaaactgtct aaggatccca acgagaaacg cgatcacatg gtgttgctcg
     2041 aatttgtgac tgctgcaggc attacccatg gaatggacga actgtacaaa taattggttt
     2101 aactcagcca gtaaaaatcc cccattaacc taggttttgg gggaaattga tcagattggc
     2161 ggatttggcc ggggttcagg ttgtaaaccg ttttgtgaaa aaatttttaa aataaaaaag
     2221 gggacctcta gggtccccaa ttaattagta atataatcta ttaaaggtca ttcaaaaggt
     2281 catccaccgg atcagcttag taaagccctc gctagatttt aatgcggatg ttgcgattac
     2341 ttcgccaact attgcgataa caagaaaaag ccagcctttc atgatatatc tcccaatttg
     2401 tgtagggctt attatgcacg cttaaaaata ataaaagcag acttgacctg atagtttggc
     2461 tgtgagcaat tatgtgctta gtgcatctaa cgcttgagtt aagccgcgcc gcgaagcggc
     2521 gtcggcttga acgaattgtt agacattatt tgccgactac cttggtgatc tcgcctttca
     2581 cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc
     2641 caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca
     2701 ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa
     2761 tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc
     2821 atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga
     2881 gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga
     2941 tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct
     3001 gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt
     3061 gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag
     3121 tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg
     3181 taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca
     3241 aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata
     3301 gttgagtcga tacttcggcg atcaccgctt ccctcatgat gtttaacttt gttttagggc
     3361 gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt
     3421 aacgcgcttg ctgcttggat gcccgaggca tagactgtac cccaaaaaaa cagtcataac
     3481 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc
     3541 agttgcgtga gcgcatacgc tacttgcatt acagcttacg aaccgaacag gcttatgtcc
     3601 actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca
     3661 gcgaagtcga ggcatttctg tcctggctgg cgaacgagga ttgaattaca aatgctagtg
     3721 ggtcagactc cagcaactat gattaggggt tcgagtttcc ctacaggaag caatcaaagc
     3781 ctcgatcacc cacagccaga actgggcaat attaccagct agacttggtt acaccgggca
     3841 aaagaccctg gtgggcccat tcccgtaaaa cattgcggca aaggccaaag tcacggtagt
     3901 aggaacgggg acgacctgta acctgacagc ggttacggcg acggttgggg gcgctattgc
     3961 ggggtaaatc ctgcaatttt tggtgaacag ctaatttgtc ttctagggtt tcagcttgac
     4021 ggaattcttc tttgagcgct tcccgtttag cagcatactt tgctaccaag cggctacgac
     4081 gcttatcccg ctcgatcatg gatttttttg ccatcggggt ttaactttat ttctttagtc
     4141 taaaaattga gcactcccca tcatatcaca ctgcttgggg acacaaaggt ttatgaaagg
     4201 taatggaact agacgaacac ggagggattc gaacccccga ccctcaggac cggaacctga
     4261 tgctctatcc actgagctac gtgtccagga cagacttggg ctgacccttt catcttgcga
     4321 aaagaattca caccagctat cagcgtaaca tttcctgcac cgattcggaa gctaactggg
     4381 caccattttc cagtaaccaa gcttcctgac tgggagctaa ccccggattg tcaccaaaac
     4441 tgagattttc cacctttgcg ccactgaaat tgacgttttg gagagtggct ccccgcaaat
     4501 ccgcttcccg taggtctgtg ccgattaaag ttgctagggc taaactactt ttacgaaaat
     4561 caacttggct gagattagcc ccttctaggt aagcgccact caggtctgca ccccgaaaat
     4621 tggaccggcg gagatcggta ttactcaaat cgctgtcggt cagaatagct ccccggaaat
     4681 tgctgtccat tagttggcca ttactaaggt caatgctgtt gaggttagca cccacaaatt
     4741 tgcctcccgc caaatcccgt tgcgggtcca aaccgactaa cttccccagg gtaaggcaat
     4801 ccaactgggg ggagttgatg attaattgca ggcgatcgcc aaggttcccg gtgtctgctt
     4861 ccgttacggt gcgattattt tcgacattgt atagaggttg ccagataatt tgaggatcgg
     4921 catcgactga tttgctcagg agccattgac cccaacaaag ggccggagtt aggtaatttt
     4981 ccgctataaa cttacatgca agcttgagta ttctatagtg tcacctaaat agcttggcgt
     5041 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5101 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5161 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     5221 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5281 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5341 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5401 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5461 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5521 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5581 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5641 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5701 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5761 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5821 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5881 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5941 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6001 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6061 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6121 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6181 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6241 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6301 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6361 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6421 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6481 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     6541 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6601 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6661 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     6721 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     6781 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     6841 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     6901 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     6961 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7021 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7081 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7141 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7201 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     7261 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     7321 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     7381 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     7441 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     7501 tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     7561 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     7621 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     7681 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     7741 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     7801 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     7861 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     7921 caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg
     7981 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     8041 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     8101 tgtaaaacga cggccagtga attg
//
