LOCUS       sequence-207560-        8975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  contains CurT-mTurquoise2 construct used to replace the native CurT 
            in Synechocystis PCC 6803 via homologous recombination..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Gutu A, Chang F, O'Shea EK
  TITLE     Dynamical localization of a thylakoid membrane binding protein is 
            required for acquisition of photosynthetic competency.
  JOURNAL   Mol Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912.
  PUBMED    29357135
REFERENCE   2  (bases 1 to 8975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912."
COMMENT     Sequence Label: pAGH23
FEATURES             Location/Qualifiers
     source          1..8975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(2135..2140,2141..2157,2158..2163)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2352..2885
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     primer_bind     complement(4893..4912)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             6370..6561
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(6555..6577)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6663..6803
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6718..6737
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6818..6835)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6989..7577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7069..7088)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7748..8539,8540..8608))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8371..8390
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8609..8713)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8781..8799
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(8826..8831,8832..8848,8849..8854)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 gccggtactg ccgggcctct tgcgggatat tacttgccat ggccagttcc cccagaccca
       61 ctgaacaaac caaacaattt tttcagcaat ggcaaaccta tcagcagttg gttgaccaca
      121 attacatggc ccatcggcaa attcaccagg cggtgggggc tttgattcaa caacagttcg
      181 atcgcccgtt tgattttttg gaccttggct gtggggatgc ccaggcgatc gccaaaactt
      241 tgacggggag taaaatcaat tcctatacag gggtagattt atcccccaat gccctggccc
      301 tagcccagaa aaatttggac ccttttcgac aggtggagct aagggaaacg gattttttga
      361 cctatctggg tgaagccaac caaaccgccc cagccagctt tgatctgatt cacgtcggtt
      421 tttctttgca ccatctgctc ccagaagaaa aggaacaatt ttttgcccat tgcttttctc
      481 tgctgcgccc tgggggatgc ctagttatct acgacgtttt tcgtcagccc gagcaaaatc
      541 gacagcagta tattgaagcc tatttagcta aggccgaaag tcaatggact gcccttgccc
      601 ccgaccaact ccaggcgatc gctgcccata taaaggagtg cgatttcccc gaattggtgg
      661 ctaccatggc agaaattggc caaaaagtag gatttatacc accccaagtc ctctttactg
      721 agcctagggg tttccaccac tgctttgcct ttgccattag tcaaagtgga aaagccctgg
      781 cccaagacgg ggtgtaaaaa gcaattttcc gataatatgg gcagaagcta aacagtgccc
      841 caatatggga agctgtgggc cgtaaacatt caatccgaat aataaaggac tggttgaaga
      901 tggaagaaca aaagacagca actgccggga ttaaaaccga tgtaggacca atcactactc
      961 ccaatcccca aaagtcccca atcaccgacc aggcttggca ggaatggcta caaccggtgt
     1021 gggaagtgct gggtaaaatt cctcaaatga ccggggagtt tttcgaggac aacaagcaac
     1081 ccctcatttc cctgggcata atcctgttgg gcattatcag cgtcaaaatc ttgattgctg
     1141 tgctcgatgc cattaatgat attcctttat tggcaccaac tttacagttg atcggcatgg
     1201 gctacaccgc ttggtttatc tggcgttacc tttggaaagc tgaaaagcgc caagagttgg
     1261 cttccgaatt tggtgcacta aaggagcaaa tttttggcgg tggaagcggc agtggtatgg
     1321 tttcgaaggg cgaagaactg tttacgggag tggtgccgat cctggttgag ttggatggcg
     1381 acgtgaacgg acacaagttt agtgtctccg gcgaaggtga gggtgacgct acctacggta
     1441 aactgacgct gaaattcatt tgtactaccg gtaaactccc ggttccatgg cctacgctgg
     1501 ttacgaccct cagctggggt gtccaatgct tcgcccgcta cccggaccac atgaagcagc
     1561 acgacttttt caaaagtgcc atgcctgagg ggtacgttca agaacgcacc attttcttta
     1621 aggatgacgg caattacaaa acacgcgcag aggtgaaatt tgaaggtgat acgttggtca
     1681 atcgaatcga actcaaaggc attgacttta aagaggatgg caatatcctc ggccacaaac
     1741 tggaatacaa ttacttcagc gacaacgtct atatcacagc tgataagcag aaaaatggga
     1801 ttaaagccaa ctttaagatc cggcataaca ttgaagatgg cggcgtgcaa ctggcggatc
     1861 attatcagca gaatactccc atcggcgatg ggcccgtact gttgccagat aaccattatc
     1921 tgtctaccca aagcaagttg tcgaaggatc ccaacgagaa gcgtgatcat atggtcctct
     1981 tggagttcgt gactgcggca ggcattaccc tcgggatgga tgaattgtat aagtaggctc
     2041 gttcgccagc caggacagaa atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg
     2101 cacaccgtgg aaacggatga aggcacgaac ccagttgaca taagcctgtt cggttcgtaa
     2161 actgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     2221 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttgtacagtc
     2281 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     2341 gagcagcaac gatgttacgc agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     2401 caaagttagg tggctcaagt atgggcatca ttcgcacatg taggctcggc cctgaccaag
     2461 tcaaatccat gcgggctgct cttgatcttt tcggtcgtga gttcggagac gtagccacct
     2521 actcccaaca tcagccggac tccgattacc tcgggaactt gctccgtagt aagacattca
     2581 tcgcgcttgc tgccttcgac caagaagcgg ttgttggcgc tctcgcggct tacgttctgc
     2641 ccaagtttga gcagccgcgt agtgagatct atatctatga tctcgcagtc tccggcgagc
     2701 accggaggca gggcattgcc accgcgctca tcaatctcct caagcatgag gccaacgcgc
     2761 ttggtgctta tgtgatctac gtgcaagcag attacggtga cgatcccgca gtggctctct
     2821 atacaaagtt gggcatacgg gaagaagtga tgcactttga tatcgaccca agtaccgcca
     2881 cctaacaatt cgttcaagcc gagatcggct tcccggccgc ggagttgttc ggtaaattgt
     2941 cacaacgccg ctgtctccag accgccccag cctctttttc ctgcgcctat cctccgggtt
     3001 tgatgttcgg gggatttttt ctggcccatg accaaccgtc ccgtcccatc ggtgtgggat
     3061 agttctccct tcattggtat tctaggagtt agtttttcgc cattcttcct cccctttttt
     3121 caggagcgat gagcaactcc ccgtctgttc ttacagagct attgcaggct ttacccaatc
     3181 tgcgggccca aacctacttc aagtcttccc tcacggcctt gtcccacgcc atggaagacc
     3241 aggtactagc ggaaagtaca gcgggcaatc ccctgatcat tgcggctttt cagcgggaga
     3301 gattttatcg tcaagaagcc caccgttatc gacgtattgc cgataagagc agtcaggttt
     3361 acgtgctctc cgctccggag acggaattta aacacaactc cggcatttac gaaaccattg
     3421 cctttactcc tgaagacagc ctcgcccagg aatggcattt gattgtcatt ggcgatcgtt
     3481 atgccagttg tctaatttgt cgagaacgac cgacccagga taaatgggat agtaaccgcc
     3541 gttttgaagg tgtttggacc tttgatcgcc aggtgagtcg agttgcagcc caactattgc
     3601 tacaacggat tttagcctac cgtccagagt tacaagccaa aatcgaggag gcgacccaaa
     3661 cctggctcca gccttcctgc actgttcccc cccggggcat tgacgataac cctgatccct
     3721 ttgtacaacg gttaatcacc tatctccaag ctggacagta taagttaatc aaagccaatc
     3781 gtaatttgca ggacaaagaa gagcgggaga gactgcttaa ctccatcaca ggggcggtgc
     3841 gacgttccct tgatcccgag gaaattatgc aggtggcggt ggaaaaacta ggccaaggtt
     3901 tgggcatttg ccgttgtgtg atttaccgct gtagtgctga tgatacagtg gcgaccatta
     3961 accatgaatt cctcggtagc ggtgttggtt ccatcaaggg ttatccctgg cccctcaaag
     4021 ataatcccct ttttcaggag gtggtggtcc tcaaggaggc gatcgccatc gacaacatcg
     4081 accgagatcc aaggttaggg gaaccggagc cttctacggg ggaagtgaaa accctggtga
     4141 agagttgtgc cattctgtcc tggatgttag tgccaatttt ttatcgggaa aggttgctgg
     4201 gcatgatgga atttcaccac tgtggtcctg cccccacgga gtgggaggac gatgccctta
     4261 ccctagtgga aggggtggcc acccaaattg gggtagcact gatccaagca gaatcctatg
     4321 ccaacctaca ggaattgaat gagcaattag ctgccctaga ccgcactcgg tctaacctgg
     4381 tggccattac agggcatgaa ctacgcaccc ccctttccac tatccaagtg tgtttggaaa
     4441 gcctggccac ggagccggat atgccccagg aattgagaca ggtaatgttg aacactgccc
     4501 tggaagatgc ggagaggatg cgcaaactgg tgcaggactt tctcacgcta tctcaactgg
     4561 aaagtggacg agtggaatgg aacgcagagc caatttccct agaagaatgt gtggagcttt
     4621 ccttgagcca tatccgagcc cacaatcggg gccaggaagt acctgtcatt gtcaatcagg
     4681 tgaatgggcg ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg gagcactgtc
     4741 cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta cttggagcca ctatcgacta
     4801 cgcgatcatg gcgaccacac ccgtcctgtg gatcctctac gccggacgca tcgtggccgg
     4861 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
     4921 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
     4981 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
     5041 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
     5101 agagcgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
     5161 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
     5221 ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat
     5281 gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac
     5341 tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga
     5401 cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat
     5461 gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca
     5521 ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac
     5581 ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa
     5641 cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg
     5701 cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga
     5761 ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc
     5821 aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc
     5881 tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc
     5941 cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga
     6001 acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc
     6061 ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc
     6121 cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg
     6181 aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt
     6241 tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg
     6301 agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg
     6361 gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc
     6421 cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc
     6481 tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt
     6541 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     6601 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     6661 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     6721 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     6781 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6841 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6901 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6961 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7021 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7081 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7141 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7201 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7261 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7321 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7381 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     7441 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7501 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7561 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7621 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7681 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7741 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7801 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7861 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7921 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7981 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8041 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8101 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8161 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8221 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8281 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8341 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     8401 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8461 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8521 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8581 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8641 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8701 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     8761 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattct
     8821 catgtttgac agcttatcat cgataagctt taatgcggta gtttatcaca gttaaattgc
     8881 taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc
     8941 gtcaccctgg atgctgtagg cataggcttg gttat
//
