LOCUS       sequence-207562-        8583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HU-FRB construct (anchor) expressed in trans from the slr0168 
            neutral site of Synechocystis PCC6803. Used to anchor away any FKBP 
            tagged target in presence of rapamycin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8583)
  AUTHORS   Gutu A, Chang F, O'Shea EK
  TITLE     Dynamical localization of a thylakoid membrane binding protein is 
            required for acquisition of photosynthetic competency.
  JOURNAL   Mol Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912.
  PUBMED    29357135
REFERENCE   2  (bases 1 to 8583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912."
COMMENT     Sequence Label: pAGH51
FEATURES             Location/Qualifiers
     source          1..8583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1349..2164)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFLLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2072..2091
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2156..2175
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(4501..4520)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             5978..6169
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(6163..6185)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6271..6411
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6326..6345
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6426..6443)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6597..7185)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6677..6696)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7356..8147,8148..8216))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7979..7998
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8217..8321)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8389..8407
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(8434..8439,8440..8456,8457..8462)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 gccggtactg ccgggcctct tgcgggattc ggcgataatc tcccctgggt cggtttttga
       61 ctggggaaag aagagaaaca cggcctgtgc tagtatctaa ggcatgaatt ttcgtgaagt
      121 taattataac agtcagtatt ttgcggctgg ctaggtgttt aggagtggca acagcatcat
      181 gacgagccat cagctcaacg ggcaacggag ttaccttcag ccgatccgca tcggggtgat
      241 tggggtgggc aatatgggac agcaccatac cagagtcctg agcctgatga aggatgtgga
      301 gtttgtgggt attgccgatg tcaatgtgga acggggccta gacaccgcca gtaagtatcg
      361 ggtgcatttt tttgaagatt atcaggagat gttgccccat gtggatgcgg tttgtgtggc
      421 ggttcccact aggctccatc acgatgtggg catgaattgt ttgcaaaata atgtccatac
      481 tctgattgaa aaacccattg ccgctagcat tgctgaagcg gaatccctgg ttaatgccgc
      541 cgccgatgcc aattgcattc tccaagtggg gcacattgaa cgcttcaacc cggcattttt
      601 agagctaacc aaaattctca aaacggaaga gttattggcg atcgaagccc atcgcatgag
      661 tccctattcc cagcgggcca atgatgtctc cgtggtattg gatttgatga tccatgacat
      721 tgacctgttg ctggaattgg tgggttcgga agtggttaaa ctgtccgcca gtggcagtcg
      781 ggcttctggg tcaggatatt tggattatgt caccgctacg ttaggcttct cctccggcat
      841 tgtggccacc ctcaccgcca gtaaggtcac ccatcgtaaa attcgttcca tcgccgccca
      901 ctgcaaaaat tccctcaccg aagcggattt tctcaataac gaaattttga tccatcgcca
      961 aaccaccgct gattggagcg cggactatgg ccaggtattg tatcgccagg atggtctaat
     1021 cgaaaaggtt tacaccagta atattgaacc tctccacgct gaattagaac attttattca
     1081 ttgtgttagg ggaggtgatc aaccctcagt ggggggagaa caggccctca aggccctgaa
     1141 gttagccagt ttaattgaag aaatggccct ggacagtcag gaatggcatg ggggggaagt
     1201 tgtgacagaa tatcaagatg ccaccctggc cctcagtgcg agtgtttaaa tcaacttaat
     1261 taatgcaatt attgcgagtt caaactcatt tctgtcctgg ctggcgaacg agccgctgcc
     1321 agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact
     1381 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     1441 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     1501 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     1561 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca
     1621 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     1681 caaccaaacc gttattcatt cgtgattgcg cctgagcgag aagaaatacg cgatcgctgt
     1741 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     1801 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg
     1861 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     1921 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     1981 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc
     2041 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     2101 cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc
     2161 tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata
     2221 tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg ctttgttgaa
     2281 taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac agggtaaaat
     2341 ccaagttttc gtaaaggatt gtgacaaaat cccgcaaaac tgcaaaaatt cgtggggaaa
     2401 agtgatccta cttccacact ttgtgcatag aataaggcag tgaaacagcc agtctgcggg
     2461 gatttcaggg gtttttaatg ccaaatttct gtgtctatcc gggctgtgga gcctcccgat
     2521 gaccctgtta tggggagaaa atgcctaaaa aatgagtcta ttccaacaaa ctcactctca
     2581 aggagatttt ttcagatgaa taaaggcgaa ttagtccatg cggtcacgga caaagcgaaa
     2641 gagcaactgg gggaaagcat cactaaaaag gaggtagatg cggtaatttc agccgccatt
     2701 gattgcatca tggaagtggt ggccgaaggg gaaaaagtaa ccctagtggg ctttggttcc
     2761 tttgaagcca gggaacgtaa ggaacgggag ggccgtaacc ccaaaactgg ggataaaatg
     2821 ctcattccag ccactaaggt gccggctttc tccgcaggca aaatgttcaa agataaagtt
     2881 gctcctgaga aaaaaggaag tggtatgtgg catgaagggc tagaagaggc atctcgtcta
     2941 tattttggcg agcggaatgt taaaggcatg tttgaagttt tggaacctct gcatgctatg
     3001 atggaacgcg gtccccaaac attaaaggaa accagtttta accaagcata tgggcgtgat
     3061 ttaatggaag cccaggaatg gtgtcgcaag tatatgaagt ctggtaatgt taaggatctg
     3121 ttgcaagcct gggatctata ctatcatgtg ttccgccgta ttagtaagta attggtttaa
     3181 ctcagccagt aaaaatcccc cattaaccta ggttttgggg gaaattgatg ggatttggcc
     3241 ggggttcagg acagctatga cctgacttac atcgaagatc atgacaacta ttacgacatt
     3301 atcctcaaag gggacgaagc cgcagttcgc caaattaaga gggttgcttt gccctccgaa
     3361 ggggattatt cggcggttta taatcccggt ggccccggca atgatccaga gaatggtccc
     3421 ccagggccct ttactgtgtc cagtagtccc caggtaattg aggtaacgga taccatcggc
     3481 cagcccacca aagtctccta tgtggaagtg gatggccccg tattgcgtaa tcccttcagt
     3541 ggtactccca ttgggcaaga ggtgggttta gcggttaaag atctggccac aggtcatgaa
     3601 atttatcagt acactgaccc agatgggaag gtattttatg cttcctttgc tgccgctgat
     3661 gaccaagcca cggatttaac cacggcgatc gccaatccca cggccatcga tttaattaac
     3721 gccaggggat ttacggcggg tagttccgtc accgtatcgg gttcctacag tcgggaagcc
     3781 ttttttgatg gatccatggg tttttatcga cttctggacg ataacggtgc agtgctagat
     3841 cccttaacag gtggtgtaat caacccagga caggtaggtt atcaagaagc agctttggca
     3901 gatagcaatc gtttgcaagc cactggctcc accctaacgg cagaagacct agaaaccaga
     3961 gcattttcct tcaatatttt gggtggcgag ttgtatgcgc catttttaac ggttaatgac
     4021 agtctttccg gtattaatca gacttatttt gcctttgggt cggccaaccc agatggcatc
     4081 agccacagca caaacttggg acccaacgtg attggttttg aagattttct cggcggagga
     4141 gacctagatt tcgacgacat cattgtcaga tttacgctga cttagctcag gggcattatc
     4201 ggagcaagcc gatattcagg taagatgggg cggtgctttt gtgggttgat ccattagctt
     4261 taaccccatt gtctccactg acggtaatag ttttcccgct atatgcgttg atgcaatttc
     4321 tatgcgcacc cgttctcgga gcactgtccg accgctttgg ccgccgccca gtcctgctcg
     4381 cttcgctact tggagccact atcgactacg cgatcatggc gaccacaccc gtcctgtgga
     4441 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
     4501 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
     4561 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
     4621 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
     4681 gcttcctaat gcaggagtcg cataagggag agcgtcgacc gatgcccttg agagccttca
     4741 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     4801 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
     4861 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
     4921 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
     4981 aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga
     5041 cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc
     5101 ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag
     5161 gatcgctcgc ggctcttacc agcctaactt cgatcattgg accgctgatc gtcacggcga
     5221 tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc gccgccctat
     5281 accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa
     5341 tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt
     5401 cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc
     5461 catctccagc agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc cacgggtgcg
     5521 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta
     5581 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc
     5641 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg
     5701 gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc
     5761 ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct
     5821 ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc
     5881 atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac
     5941 ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc
     6001 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga
     6061 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct
     6121 ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     6181 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     6241 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     6301 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     6361 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     6421 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6481 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6541 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6601 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6661 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6721 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6781 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6841 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6901 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6961 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7021 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7081 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7141 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7201 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7261 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7321 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7381 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7441 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7501 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7561 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7621 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
     7681 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7741 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7801 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7861 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7921 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     7981 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8041 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8101 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8161 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8221 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8281 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8341 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     8401 acgaggccct ttcgtcttca agaattctca tgtttgacag cttatcatcg ataagcttta
     8461 atgcggtagt ttatcacagt taaattgcta acgcagtcag gcaccgtgta tgaaatctaa
     8521 caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt
     8581 tat
//
