LOCUS       sequence-207565-        7714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express cytoplasmic tail human PC1 from amino acid 4077 to 4302 with
            a membrane anchor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Lin CC, Kurashige M, Liu Y, Terabayashi T, Ishimoto Y, Wang T, 
            Choudhary V, Hobbs R, Liu LK, Lee PH, Outeda P, Zhou F, Restifo NP, 
            Watnick T, Kawano H, Horie S, Prinz W, Xu H, Menezes LF, Germino GG
  TITLE     A cleavage product of Polycystin-1 is a mitochondrial matrix protein
            that affects mitochondria morphology and function when 
            heterologously expressed.
  JOURNAL   Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1038/s41598-018-20856-6.
  PUBMED    29426897
REFERENCE   2  (bases 1 to 7714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-02-9"; volume: "8(1):2743. doi"; pages: " 
            10.1038/s41598-018-20856-6"
COMMENT     Sequence Label: pcDNA3-CD16.7-hPC1-eGFP-3HA
FEATURES             Location/Qualifiers
     source          1..7714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(2436..2438,2439..2441,2442..3152)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2436..2438,2439..2441,2442..3152)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2481..2502)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2742..2761)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3089..3110
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3162..3188
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3192..3218
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3222..3248
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3267..3285)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3268..3285)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3305..3322)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3311..3535
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3581..4009
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3668..3687)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3878..3899
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4018..4038)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4023..4352
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4203..4338
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4265..4284
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4419..5213
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4473..4492)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5083..5102
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5387..5508
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5424..5443)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5478..5497
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5557..5573)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5557..5573)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5570..5592)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5581..5597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5605..5611,5612..5629,5630..5635))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5650..5671
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5788..5805)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5959..6547)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6039..6058)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6718..7509,7510..7578))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7341..7360
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7579..7683)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa taccatgtgg cagctgctcc
      961 tcccaactgc tctgctactt ctagtttcag ctggcatgcg gactgaagat ctcccaaagg
     1021 ctgtggtgtt cctggagcct caatggtaca gcgtgcttga gaaggacagt gtgactctga
     1081 agtgccaggg agcctactcc cctgaggaca attccacaca gtggtttcac aatgagagcc
     1141 tcatctcaag ccaggcctcg agctacttca ttgacgctgc cacagtcaac gacagtggag
     1201 agtacaggtg ccagacaaac ctctccaccc tcagtgaccc ggtgcagcta gaagtccata
     1261 tcggctggct gttgctccag gcccctcggt gggtgttcaa ggaggaagac cctattcacc
     1321 tgaggtgtca cagctggaag aacactgctc tgcataaggt cacatattta cagaatggca
     1381 aagacaggaa gtattttcat cataattctg acttccacat tccaaaagcc acactcaaag
     1441 atagcggctc ctacttctgc agggggcttg ttgggagtaa aaatgtgtct tcagagactg
     1501 tgaacatcac catcactcaa ggtttggcag tgtcaaccat ctcatcattc tctccacctg
     1561 gcgcggatcc accaagggcc tctgccctcc ctgccccacc gacaggctcc gccctccctg
     1621 acccgcagac agcctctgcc ctccctgacc cgccagcagc ctctgccctc cctgcggccc
     1681 tggcggtgat ctccttcctc ctcgggctgg gcctgggggt ggcgtgtgtg ctggcgagga
     1741 cgcgtgagtc ctggcacttg tcacccctgc tgtgtgtggg gctctgggca ctgcggctgt
     1801 ggggcgccct acggctgggg gctgttattc tccgctggcg ctaccacgcc ttgcgtggag
     1861 agctgtaccg gccggcctgg gagccccagg actacgagat ggtggagttg ttcctgcgca
     1921 ggctgcgcct ctggatgggc ctcagcaagg tcaaggagtt ccgccacaaa gtccgctttg
     1981 aagggatgga gccgctgccc tctcgctcct ccaggggctc caaggtatcc ccggatgtgc
     2041 ccccacccag cgctggctcc gatgcctcgc acccctccac ctcctccagc cagctggatg
     2101 ggctgagcgt gagcctgggc cggctgggga caaggtgtga gcctgagccc tcccgcctcc
     2161 aagccgtgtt cgaggccctg ctcacccagt ttgaccgact caaccaggcc acagaggacg
     2221 tctaccagct ggagcagcag ctgcacagcc tgcaaggccg caggagcagc cgggcgcccg
     2281 ccggatcttc ccgtggccca tccccgggcc tgcggccagc actgcccagc cgccttgccc
     2341 gggccagtcg gggtgtggac ctggccactg gccccagcag gacacccctt cgggccaaga
     2401 acaaggtcca ccccagcagc actctcgaga tcgatatggt gagcaagggc gaggagctgt
     2461 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     2521 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     2581 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     2641 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     2701 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     2761 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     2821 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     2881 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     2941 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     3001 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     3061 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     3121 ggatcactct cggcatggac gagctgtaca agatcgatat gtacccatac gatgttccag
     3181 attacgctgg ctatccctat gacgtcccgg actatgcagg atatccatat gacgttccag
     3241 attacgctta atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga
     3301 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     3361 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     3421 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     3481 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     3541 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     3601 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3661 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3721 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3781 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3841 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3901 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     3961 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     4021 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4081 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     4141 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4201 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4261 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     4321 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     4381 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     4441 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     4501 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     4561 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     4621 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     4681 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     4741 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     4801 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     4861 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     4921 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     4981 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     5041 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     5101 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     5161 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     5221 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     5281 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     5341 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     5401 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5461 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     5521 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     5581 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     5641 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     5701 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5761 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5821 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5881 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5941 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6001 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6061 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6121 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6181 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6241 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6301 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6361 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6421 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6481 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6541 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     6601 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     6661 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     6721 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     6781 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     6841 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     6901 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     6961 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7021 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7081 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7141 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7201 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7261 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     7321 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7381 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7441 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7501 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7561 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     7621 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     7681 gcgcacattt ccccgaaaag tgccacctga cgtc
//
