LOCUS       sequence-207577-        4711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Modafferi EF, Black DL
  TITLE     A complex intronic splicing enhancer from the c-src pre-mRNA 
            activates inclusion of a heterologous exon.
  JOURNAL   Mol Cell Biol. 1997 Nov . 17(11):6537-45.
  PUBMED    9343417
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1997 Nov . 17(11):6537-45."
COMMENT     Sequence Label: pDUP 4-1
FEATURES             Location/Qualifiers
     source          1..4711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(join(1810..1816,1817..1834,1835..1840))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1855..1876
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1993..2010)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2164..2752)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2244..2263)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2923..3714,3715..3783))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3546..3565
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3784..3888)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3956..3974
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4012..4034)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4134..4153
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4367..4386
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        4676..4694
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4676..4693
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatca gcttgcttac
      541 atttgcttct gacacaactg tgttcactag caacctcaaa cagacaccat gcatggtgca
      601 cctgactcct gaggagaagt ctgccgttac tgccctgtgg ggcaaggtga acgtggatga
      661 agttggtggt gaggccctgg gcaggttggt atcaaggtta caagacaggt ttaaggagac
      721 caatagaaac tgggcatgtg gagacagaga agactcttgg gtttctgata gggcccactg
      781 actctctctg cctattggtc tattttccca cccttaggct gctggtggtg ccatggcagg
      841 ccctgggcag gttggtatca aggttacaag acaggtttaa ggagaccaat agatctgggc
      901 atgtggagac agagaagact cttgggtttc tgataggcac tgactctctc tgcctattgg
      961 tctattttcc cacccttagg ctgctggtgg tctacccttg gacccagagg ttctttgagt
     1021 cctttgggga tctgtccact cctgatgctg ttatgggcaa ccctaaggtg aaggctcatg
     1081 gcaagaaagt gctcggtgcc tttagtgatg gcctggctca cctggacaac ctcaagggca
     1141 cctttgccac actgagtgag ctgcactgtg acaagctgca cgtggatcct tgagcatctg
     1201 gattctgcct aataaaaaac atttattttc attgcaatga tgtatttaaa ttatttctga
     1261 atattttact aaaaagggaa tgtgggaggt cagtgcattt aaaacataaa gaaatgaaga
     1321 gctagttcaa accttgggaa aatacactat atcttaaact ccatgaaaga aggtgaggct
     1381 gcaaacagct aatgcacatt ggcaacagcc ctgatgccta tgccttattc atccctcaga
     1441 aaaggattca agtagaggct tgatttggag gttaaagttt tgctatgctg tattttacat
     1501 tacttattgt tttagctgtc ctcatgaatg tcttttcact acccatttgc ttatcctgca
     1561 tctctcagcc ttgactccac tcagttctct tgcttagaga taccaccttt cccctgaagt
     1621 gttccttcca tgttttacgg cgagatggtt tctcctcgcc tggccactca gccttagttg
     1681 tctctgttgt cttatagagg tctacttgaa gaaggaaaaa cagggggcat ggtttgactg
     1741 tcctgtgagc ccttcttccc tgcctccccc actcacagtg acccggaatc atccgctcac
     1801 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     1861 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     1921 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1981 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2041 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2101 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2161 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2221 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2281 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2341 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2401 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2461 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2521 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2581 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2641 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2701 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2761 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2821 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2881 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2941 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3001 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3061 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3121 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3181 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3241 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3301 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3361 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3421 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3481 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3541 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3601 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3661 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3721 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3781 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3841 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3901 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     3961 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     4021 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     4081 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     4141 agagcagatt gtactgagag tgcaccattc gacgctctcc cttatgcgac tcctgcatta
     4201 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
     4261 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     4321 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     4381 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     4441 agaggatctg gctagcgatg accctgctga ttggttcgct gaccatttcc gggtgcggga
     4501 cggcgttacc agaaactcag aaggttcgtc caaccaaacc gactctgacg gcagtttacg
     4561 agagagatga tagggtctgc ttcagtaagc cagatgctac acaattaggc ttgtacatat
     4621 tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta
     4681 ggtgacacta tagaatacaa gctgatccgt c
//
