LOCUS       sequence-20758-e         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG416GAL-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(128..252)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             311..388
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             416..436
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tctcgttatc ggggaaaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
       61 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
      121 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
      181 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt
      241 gtacaaagtg gtgatgggct gcaggaattc gatatcaagc ttatcgatag gtggaccagg
      301 tggtggaatg aagcgacgat ggaaaaagaa tttcatagcc gtctcagcag ccaaccgctt
      361 taagaaaatc tcatcctccg gggcacttga ttatggtacc ccaactactg cttctgaaaa
      421 tctatatttt caaggtgaac taaaaactgc tgctttggct caacatgcga ttaagggtga
      481 agctcaaaaa cttaatgact ctcaagctcc aaaagctgat gcgcaacaaa ataacttcaa
      541 caaagatcaa caaagcgcct tctatgaaat cttgaacatg cctaacttaa acgaagcgca
      601 acgtaacggc ttcattcaaa gtcttaaaga cgacccaagc caaagcacta acgttttagg
      661 tga
//
