LOCUS       sequence-207608-       11180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9 gap repair vector - NatR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11180)
  TITLE     Ellis Lab Yeast CRISPR Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pWS174
FEATURES             Location/Qualifiers
     source          1..11180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             767..4870
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             767..4870
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4883..4903
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4907..4927
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     RBS             5837..5848
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5855..6568
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      6580..6651
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6667..6694
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_RNA        6708..6783
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      8215..8412
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      8417..9399
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(9457..10272)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9457..10272)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10180..10199
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10264..10283
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10497..11085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10986..11005
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgtgcggcc gcccctgaat tcgcatctag actgaactgg ccgataattg cagacgaacg
       61 gtgagtaagg aaagagtgag gaactatcgc atacctgcat ttaaagatgc cgatttgggc
      121 gcgaatcctt tattttggct tcaccctcat actattatca gggccagaaa aaggaagtgt
      181 ttccctcctt cttgaattga tgttaccctc ataaagcacg tggcctctta tcgagaaaga
      241 aattaccgtc gctcgtgatt tgtttgcaaa aagaacaaaa ctgaaaaaac ccagacacgc
      301 tcgacttcct gtcatcctat tgattgcagc ttccaatttc gtcacacaac aaggtcctag
      361 cgacggctca caggttttgt aacaagcaat cgaaggttct ggaatggcgg gaaagggttt
      421 agtaccacat gctatgatgc ccactgtgat ctccagagca aagttcgttc gatcgtactg
      481 ttactctctc tctttcaaac agaattgtcc gaatcgtgtg acaacaacag cctgttctca
      541 cacactcttt tcttctaacc aagggggtgg tttagtttag tagaacctcg tgaaacttac
      601 atttacatat atataaactt gcataaattg gtcaatgcaa gaaatacata tttggtcttt
      661 tctaattcgt agtttttcaa gttcttagat gctttctttt tctctttttt acagatcatc
      721 aaggaagtaa ttatctactt tttacaacaa atataaaaca agatctatgg acaagaagta
      781 ttctatcgga ctggatatcg ggactaatag cgtcgggtgg gccgtgatca ctgacgagta
      841 caaggtgccc tctaagaagt tcaaggtgct cgggaacacc gaccggcatt ccatcaagaa
      901 aaatctgatc ggagctctcc tctttgattc aggggaaacc gctgaagcaa cccgcctcaa
      961 gcggactgct agacggcggt acaccaggag gaagaaccgg atttgttacc ttcaagagat
     1021 attctccaac gaaatggcaa aggtcgacga cagcttcttc cataggctgg aagaatcatt
     1081 cctcgtggaa gaggataaga agcatgaacg gcatcccatc ttcggtaata tcgtcgacga
     1141 ggtggcctat cacgagaaat acccaaccat ctaccatctt cgcaaaaagc tggtggactc
     1201 aaccgacaag gcagacctcc ggcttatcta cctggccctg gcccacatga tcaagttcag
     1261 aggccacttc ctgatcgagg gcgacctcaa tcctgacaat agcgatgtgg ataaactgtt
     1321 catccagctg gtgcagactt acaaccagct ctttgaagag aaccccatca atgcaagcgg
     1381 agtcgatgcc aaggccattc tgtcagcccg gctgtcaaag agccgcagac ttgagaatct
     1441 tatcgctcag ctgccgggtg aaaagaaaaa tggactgttc gggaacctga ttgctctttc
     1501 acttgggctg actcccaatt tcaagtctaa tttcgacctg gcagaggatg ccaagctgca
     1561 actgtccaag gacacctatg atgacgatct cgacaacctc ctggcccaga tcggtgacca
     1621 atacgccgac cttttccttg ctgctaagaa tctttctgac gccatcctgc tgtctgacat
     1681 tctccgcgtg aacactgaaa tcaccaaggc ccctctttca gcttcaatga ttaagcggta
     1741 tgatgagcac caccaggacc tgaccctgct taaggcactc gtccggcagc agcttccgga
     1801 gaagtacaag gaaatcttct ttgaccagtc aaagaatgga tacgccggct acatcgacgg
     1861 aggtgcctcc caagaggaat tttataagtt tatcaaacct atccttgaga agatggacgg
     1921 caccgaagag ctcctcgtga aactgaatcg ggaggatctg ctgcggaagc agcgcacttt
     1981 cgacaatggg agcattcccc accagatcca tcttggggag cttcacgcca tccttcggcg
     2041 ccaagaggac ttctacccct ttcttaagga caacagggag aagattgaga aaattctcac
     2101 tttccgcatc ccctactacg tgggacccct cgccagagga aatagccggt ttgcttggat
     2161 gaccagaaag tcagaagaaa ctatcactcc ctggaacttc gaagaggtgg tggacaaggg
     2221 agccagcgct cagtcattca tcgaacggat gactaacttc gataagaacc tccccaatga
     2281 gaaggtcctg ccgaaacatt ccctgctcta cgagtacttt accgtgtaca acgagctgac
     2341 caaggtgaaa tatgtcaccg aagggatgag gaagcccgca ttcctgtcag gcgaacaaaa
     2401 gaaggcaatt gtggaccttc tgttcaagac caatagaaag gtgaccgtga agcagctgaa
     2461 ggaggactat ttcaagaaaa ttgaatgctt cgactctgtg gagattagcg gggtcgaaga
     2521 tcggttcaac gcaagcctgg gtacttacca tgatctgctt aagatcatca aggacaagga
     2581 ttttctggac aatgaggaga acgaggacat ccttgaggac attgtcctga ctctcactct
     2641 gttcgaggac cgggaaatga tcgaggagag gcttaagacc tacgcccatc tgttcgacga
     2701 taaagtgatg aagcaactta aacggagaag atataccgga tggggacgcc ttagccgcaa
     2761 actcatcaac ggaatccggg acaaacagag cggaaagacc attcttgatt tccttaagag
     2821 cgacggattc gctaatcgca acttcatgca acttatccat gatgattccc tgacctttaa
     2881 ggaggacatc cagaaggccc aagtgtctgg acaaggtgac tcactgcacg agcatatcgc
     2941 aaatctggct ggttcacccg ctattaagaa gggtattctc cagaccgtga aagtcgtgga
     3001 cgagctggtc aaggtgatgg gtcgccataa accagagaac attgtcatcg agatggccag
     3061 ggaaaaccag actacccaga agggacagaa gaacagcagg gagcggatga aaagaattga
     3121 ggaagggatt aaggagctcg ggtcacagat ccttaaagag cacccggtgg aaaacaccca
     3181 gcttcagaat gagaagctct atctgtacta ccttcaaaat ggacgcgata tgtatgtgga
     3241 ccaagagctt gatatcaaca ggctctcaga ctacgacgtg gaccatatcg tccctcagag
     3301 cttcctcaaa gacgactcaa ttgacaataa ggtgctgact cgctcagaca agaaccgggg
     3361 aaagtcagat aacgtgccct cagaggaagt cgtgaaaaag atgaagaact attggcgcca
     3421 gcttctgaac gcaaagctga tcactcagcg gaagttcgac aatctcacta aggctgagag
     3481 gggcggactg agcgaactgg acaaagcagg attcattaaa cggcaacttg tggagactcg
     3541 gcagattact aaacatgtcg cccaaatcct tgactcacgc atgaatacca agtacgacga
     3601 aaacgacaaa cttatccgcg aggtgaaggt gattaccctg aagtccaagc tggtcagcga
     3661 tttcagaaag gactttcaat tctacaaagt gcgggagatc aataactatc atcatgctca
     3721 tgacgcatat ctgaatgccg tggtgggaac cgccctgatc aagaagtacc caaagctgga
     3781 aagcgagttc gtgtacggag actacaaggt ctacgacgtg cgcaagatga ttgccaaatc
     3841 tgagcaggag atcggaaagg ccaccgcaaa gtacttcttc tacagcaaca tcatgaattt
     3901 cttcaagacc gaaatcaccc ttgcaaacgg tgagatccgg aagaggccgc tcatcgagac
     3961 taatggggag actggcgaaa tcgtgtggga caagggcaga gatttcgcta ccgtgcgcaa
     4021 agtgctttct atgcctcaag tgaacatcgt gaagaaaacc gaggtgcaaa ccggaggctt
     4081 ttctaaggaa tcaatcctcc ccaagcgcaa ctccgacaag ctcattgcaa ggaagaagga
     4141 ttgggaccct aagaagtacg gcggattcga ttcaccaact gtggcttatt ctgtcctggt
     4201 cgtggctaag gtggaaaaag gaaagtctaa gaagctcaag agcgtgaagg aactgctggg
     4261 tatcaccatt atggagcgca gctccttcga gaagaaccca attgactttc tcgaagccaa
     4321 aggttacaag gaagtcaaga aggaccttat catcaagctc ccaaagtata gcctgttcga
     4381 actggagaat gggcggaagc ggatgctcgc ctccgctggc gaacttcaga agggtaatga
     4441 gctggctctc ccctccaagt acgtgaattt cctctacctt gcaagccatt acgagaagct
     4501 gaaggggagc cccgaggaca acgagcaaaa gcaactgttt gtggagcagc ataagcatta
     4561 tctggacgag atcattgagc agatttccga gttttctaaa cgcgtcattc tcgctgatgc
     4621 caacctcgat aaagtcctta gcgcatacaa taagcacaga gacaaaccaa ttcgggagca
     4681 ggctgagaat atcatccacc tgttcaccct caccaatctt ggtgcccctg ccgcattcaa
     4741 gtacttcgac accaccatcg accggaaacg ctatacctcc accaaagaag tgctggacgc
     4801 caccctcatc caccagagca tcaccggact ttacgaaact cggattgacc tctcacagct
     4861 cggaggggat gagggagctg atccaaaaaa gaagagaaag gtagatccaa aaaagaagag
     4921 aaaggtataa ggatcctaac tcgagattga attgaattga aatcgataga tcaatttttt
     4981 tcttttctct ttccccatcc tttacgctaa aataatagtt tattttattt tttgaatatt
     5041 ttttatttat atacgtatat atagactatt atttatcttt taatgattat taagattttt
     5101 attaaaaaaa aattcgctcc tcttttaatg cctttatgca gttttttttt cccattcgat
     5161 atttctatgt gctgccaaga tatcaacaac agaggacata tgccctacct ccatgatcta
     5221 ctgacgtccc tgaggctgca attcatgtaa tggggcagta tccgcggcaa gtcctagtgc
     5281 aatggcggtt ttttaccctc gttctgaaga agaggcgacg cgggtgcggt catcactaat
     5341 gtggaaattg gtaagactct cgggcctccg cctttaggcg gtgcttactc tttcataaag
     5401 gggctgttag ttatgcaacg atgtgcttca gtattacatt ttttgccttc aacgccttga
     5461 ttgttctatt tttgctaata ataaatctat ttcatcggac taaaagtcca ttagttgtaa
     5521 gcggatttag ctcagttggg agagcgccag actgaagaaa aacttcggtc aagtcatctg
     5581 gaggtcctgt gttcgatcca cagaattcgc agatggccgg catggtccca gcctcctcgc
     5641 tggcgccggc tgggcaacac cttcgggtgg cgaatgggac ttgagacgga aagtgaaacg
     5701 tgatttcatg cgtcattttg aacattttgt aaatcttatt taataatgtg tgcggcaatt
     5761 cacatttaat ttatgaatgt tttcttaaca tcgcggcaac tcaagaaacg gcaggttcgg
     5821 atcttagcta ctagagaaag aggagaaata ctagatgcgt aaaggcgaag agctgttcac
     5881 tggtgtcgtc cctattctgg tggaactgga tggtgatgtc aacggtcata agttttccgt
     5941 gcgtggcgag ggtgaaggtg acgcaactaa tggtaaactg acgctgaagt tcatctgtac
     6001 tactggtaaa ctgccggttc cttggccgac tctggtaacg acgctgactt atggtgttca
     6061 gtgctttgct cgttatccgg accatatgaa gcagcatgac ttcttcaagt ccgccatgcc
     6121 ggaaggctat gtgcaggaac gcacgatttc ctttaaggat gacggcacgt acaaaacgcg
     6181 tgcggaagtg aaatttgaag gcgataccct ggtaaaccgc attgagctga aaggcattga
     6241 ctttaaagag gacggcaata tcctgggcca taagctggaa tacaatttta acagccacaa
     6301 tgtttacatc accgccgata aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca
     6361 caacgtggag gatggcagcg tgcagctggc tgatcactac cagcaaaaca ctccaatcgg
     6421 tgatggtcct gttctgctgc cagacaatca ctatctgagc acgcaaagcg ttctgtctaa
     6481 agatccgaac gagaaacgcg atcatatggt tctgctggag ttcgtaaccg cagcgggcat
     6541 cacgcatggt atggatgaac tgtacaaatg accaggcatc aaataaaacg aaaggctcag
     6601 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     6661 cacactggct caccttcggg tgggcctttc tgcgtttata cgtctcagtt ttagagctag
     6721 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     6781 tgcttttttt attttttgtc actattgtta tgtaaaatgc cacctctgac agtatggaac
     6841 gcaaacttct gtctagtgga tagctggaca ttccaggcag tgcctctgcc gccggacccc
     6901 tctcgtgatt gggtagctgg acatgccctt gtaagatata acaagagcct gcctgtctaa
     6961 tgatctcacg gcgaaagtcg gggagacagc agcggctgca gacattatac cgcaacaaca
     7021 ctaaggtgag ataactccgt acattgacta cgcgttcctc tagaccttac ttgaccggat
     7081 acagtgtctt tgacacgttt atgggttaca gcaatcacat ccaagactgg ctatgcacga
     7141 agcaactctt gagtgttaaa atgttgaccc ctgtatttgg gatgcgggta gtagatgagt
     7201 gcaggatatc agcagttaca gagatgttac gaaccactag tgcactgcag tacagtttag
     7261 cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt
     7321 gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc
     7381 atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc
     7441 aaaaattacg gctcctcgct ccagacctgc gagcagggaa acgctcccct cacagacgcg
     7501 ttgaattgtc cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg
     7561 aggttcttct ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca
     7621 tccgaacata aacaaaaatg ggtactacct tagatgatac agcctacaga tacagaacat
     7681 cagtccctgg tgatgctgaa gcaattgagg ctttagacgg ttcattcacc accgacaccg
     7741 tctttagagt aaccgccacc ggtgatggat ttaccttaag agaagtccca gtcgaccctc
     7801 cattaactaa agtctttcca gatgatgaat ctgatgacga aagcgacgac ggagaagatg
     7861 gtgacccaga ttcaagaact ttcgtagcat acggtgatga cggtgatttg gctggttttg
     7921 tagtcgtttc ttattcaggt tggaatagaa ggttgaccgt tgaagatata gaagtcgccc
     7981 cagagcatag aggtcatggt gtaggaagag ctttgatggg tttggctaca gaatttgcaa
     8041 gagagagagg agccggtcat ttatggttag aagttactaa tgttaacgcc cctgctatcc
     8101 atgcttatag aagaatgggt ttcacattat gtggtttaga tactgcttta tatgatggaa
     8161 cagcatctga cggtgaacag gccttgtata tgtctatgcc ttgcccttaa agtaactgac
     8221 aataaaaaga ttcttgtttt caagaacttg tcatttgtat agttttttta tattgtagtt
     8281 gttctatttt aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga catcatctgc
     8341 ccagatgcga agttaagtgc gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg
     8401 gtcgctatac tggagtaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
     8461 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     8521 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
     8581 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     8641 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
     8701 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
     8761 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
     8821 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
     8881 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
     8941 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
     9001 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
     9061 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
     9121 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
     9181 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
     9241 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
     9301 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcatc agagcgcttt
     9361 tggttttcaa aagcgctctg aagttcctat actttctagc tagagaatag gaacttcccg
     9421 agcggccgcg tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa
     9481 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     9541 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     9601 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     9661 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     9721 cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     9781 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgaggc gaaatacgcg
     9841 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
     9901 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     9961 tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
    10021 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
    10081 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
    10141 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
    10201 atataaatca gcatccatgt tggaatttaa tcgcggcctg gagcaagacg tttcccgttg
    10261 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
    10321 tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct
    10381 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag tcatgaccaa aatcccttaa
    10441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    10501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    10561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    10621 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    10681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    10741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    10801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    10861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    10921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    10981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    11041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    11101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    11161 tcccctgatt ctgtggataa
//
