LOCUS       sequence-207614-       10491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for human codon optimized 
            DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR 
            activation domain, encoding E174R/S542R/K548R/D908A substitutions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10491)
  AUTHORS   Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, 
            Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, 
            Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas12a variants with increased activities and 
            improved targeting ranges for gene, epigenetic and base editing.
  JOURNAL   Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0.
  PUBMED    30742127
REFERENCE   2  (bases 1 to 10491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0."
COMMENT     Sequence Label: 
            pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)
FEATURES             Location/Qualifiers
     source          1..10491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..662
                     /label=chicken beta-actin promoter
     intron          663..1671
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1595..1617
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1679..1698
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1770..5690
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     CDS             5691..5738
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5745..5825
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5856..6005
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6030..6050
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6480..6836
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             6855..7424
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     primer_bind     complement(7513..7532)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7578..7633
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7632..7651)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7994..8010)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7994..8010)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8007..8029)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8018..8034
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8042..8048,8049..8066,8067..8072))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8087..8108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8167..8362
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8213..8348
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8368..8502
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8405..8424)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8459..8478
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8570..8587)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8741..9329)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8821..8840)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9500..10291,10292..10360))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10123..10142
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10361..10465)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catgggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgttgcct tcgccccgtg ccccgctccg cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggctcgtttc ttttctgtgg ctgcgtgaaa
      841 gccttaaagg gctccgggag ggccctttgt gcggggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggcccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcgtgtgcgc gaggggagcg cggccggggg
     1021 cggtgccccg cggtgcgggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
     1141 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
     1201 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     1261 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctggcggagc cgaaatctgg gaggcgccgc cgcaccccct
     1441 ctagcgggcg cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
     1501 tcgtgcgtcg ccgcgccgcc gtccccttct ccatctccag cctcggggct gccgcagggg
     1561 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     1621 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
     1681 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattctgcag tcgacggtac
     1741 cgcgggcccg ggatccaccg gtcgccacca tgacacagtt cgagggcttt accaacctgt
     1801 atcaggtgag caagacactg cggtttgagc tgatcccaca gggcaagacc ctgaagcaca
     1861 tccaggagca gggcttcatc gaggaggaca aggcccgcaa tgatcactac aaggagctga
     1921 agcccatcat cgatcggatc tacaagacct atgccgacca gtgcctgcag ctggtgcagc
     1981 tggattggga gaacctgagc gccgccatcg actcctatag aaaggagaaa accgaggaga
     2041 caaggaacgc cctgatcgag gagcaggcca catatcgcaa tgccatccac gactacttca
     2101 tcggccggac agacaacctg accgatgcca tcaataagag acacgccgag atctacaagg
     2161 gcctgttcaa ggccgagctg tttaatggca aggtgctgaa gcagctgggc accgtgacca
     2221 caaccgagca cgagaacgcc ctgctgcgga gcttcgacaa gtttacaacc tacttctccg
     2281 gcttttatag aaacaggaag aacgtgttca gcgccgagga tatcagcaca gccatcccac
     2341 accgcatcgt gcaggacaac ttccccaagt ttaaggagaa ttgtcacatc ttcacacgcc
     2401 tgatcaccgc cgtgcccagc ctgcgggagc actttgagaa cgtgaagaag gccatcggca
     2461 tcttcgtgag cacctccatc gaggaggtgt tttccttccc tttttataac cagctgctga
     2521 cacagaccca gatcgacctg tataaccagc tgctgggagg aatctctcgg gaggcaggca
     2581 ccgagaagat caagggcctg aacgaggtgc tgaatctggc catccagaag aatgatgaga
     2641 cagcccacat catcgcctcc ctgccacaca gattcatccc cctgtttaag cagatcctgt
     2701 ccgataggaa caccctgtct ttcatcctgg aggagtttaa gagcgacgag gaagtgatcc
     2761 agtccttctg caagtacaag acactgctga gaaacgagaa cgtgctggag acagccgagg
     2821 ccctgtttaa cgagctgaac agcatcgacc tgacacacat cttcatcagc cacaagaagc
     2881 tggagacaat cagcagcgcc ctgtgcgacc actgggatac actgaggaat gccctgtatg
     2941 agcggagaat ctccgagctg acaggcaaga tcaccaagtc tgccaaggag aaggtgcagc
     3001 gcagcctgaa gcacgaggat atcaacctgc aggagatcat ctctgccgca ggcaaggagc
     3061 tgagcgaggc cttcaagcag aaaaccagcg agatcctgtc ccacgcacac gccgccctgg
     3121 atcagccact gcctacaacc ctgaagaagc aggaggagaa ggagatcctg aagtctcagc
     3181 tggacagcct gctgggcctg taccacctgc tggactggtt tgccgtggat gagtccaacg
     3241 aggtggaccc cgagttctct gcccggctga ccggcatcaa gctggagatg gagccttctc
     3301 tgagcttcta caacaaggcc agaaattatg ccaccaagaa gccctactcc gtggagaagt
     3361 tcaagctgaa ctttcagatg cctacactgg ccagaggctg ggacgtgaat agagagaaga
     3421 acaatggcgc catcctgttt gtgaagaacg gcctgtacta tctgggcatc atgccaaagc
     3481 agaagggcag gtataaggcc ctgagcttcg agcccacaga gaaaaccagc gagggctttg
     3541 ataagatgta ctatgactac ttccctgatg ccgccaagat gatcccaaag tgcagcaccc
     3601 agctgaaggc cgtgacagcc cactttcaga cccacacaac ccccatcctg ctgtccaaca
     3661 atttcatcga gcctctggag atcacaaagg agatctacga cctgaacaat cctgagaagg
     3721 agccaaagaa gtttcagaca gcctacgcca agaaaaccgg cgaccagaag ggctacagag
     3781 aggccctgtg caagtggatc gacttcacaa gggattttct gtccaagtat accaagacaa
     3841 cctctatcga tctgtctagc ctgcggccat cctctcagta taaggacctg ggcgagtact
     3901 atgccgagct gaatcccctg ctgtaccaca tcagcttcca gagaatcgcc gagaaggaga
     3961 tcatggatgc cgtggagaca ggcaagctgt acctgttcca gatctataac aaggactttg
     4021 ccaagggcca ccacggcaag cctaatctgc acacactgta ttggaccggc ctgttttctc
     4081 cagagaacct ggccaagaca agcatcaagc tgaatggcca ggccgagctg ttctaccgcc
     4141 ctaagtccag gatgaagagg atggcacacc ggctgggaga gaagatgctg aacaagaagc
     4201 tgaaggatca gaaaacccca atccccgaca ccctgtacca ggagctgtac gactatgtga
     4261 atcacagact gtcccacgac ctgtctgatg aggccagggc cctgctgccc aacgtgatca
     4321 ccaaggaggt gtctcacgag atcatcaagg ataggcgctt taccagcgac aagttctttt
     4381 tccacgtgcc tatcacactg aactatcagg ccgccaattc cccatctaag ttcaaccaga
     4441 gggtgaatgc ctacctgaag gagcaccccg agacacctat catcggcatc gcccggggcg
     4501 agagaaacct gatctatatc acagtgatcg actccaccgg caagatcctg gagcagcgga
     4561 gcctgaacac catccagcag tttgattacc agaagaagct ggacaacagg gagaaggaga
     4621 gggtggcagc aaggcaggcc tggtctgtgg tgggcacaat caaggatctg aagcagggct
     4681 atctgagcca ggtcatccac gagatcgtgg acctgatgat ccactaccag gccgtggtgg
     4741 tgctggagaa cctgaatttc ggctttaaga gcaagaggac cggcatcgcc gagaaggccg
     4801 tgtaccagca gttcgagaag atgctgatcg ataagctgaa ttgcctggtg ctgaaggact
     4861 atccagcaga gaaagtggga ggcgtgctga acccatacca gctgacagac cagttcacct
     4921 cctttgccaa gatgggcacc cagtctggct tcctgtttta cgtgcctgcc ccatatacat
     4981 ctaagatcga tcccctgacc ggcttcgtgg accccttcgt gtggaaaacc atcaagaatc
     5041 acgagagccg caagcacttc ctggagggct tcgactttct gcactacgac gtgaaaaccg
     5101 gcgacttcat cctgcacttt aagatgaaca gaaatctgtc cttccagagg ggcctgcccg
     5161 gctttatgcc tgcatgggat atcgtgttcg agaagaacga gacacagttt gacgccaagg
     5221 gcaccccttt catcgccggc aagagaatcg tgccagtgat cgagaatcac agattcaccg
     5281 gcagataccg ggacctgtat cctgccaacg agctgatcgc cctgctggag gagaagggca
     5341 tcgtgttcag ggatggctcc aacatcctgc caaagctgct ggagaatgac gattctcacg
     5401 ccatcgacac gatggtggcc ctgatccgca gcgtgctgca gatgcggaac tccaatgccg
     5461 ccacaggcga ggactatatc aacagccccg tgcgcgatct gaatggcgtg tgcttcgact
     5521 cccggtttca gaacccagag tggccaatgg acgccgatgc caatggcgcc taccacatcg
     5581 ccctgaaggg ccagctgctg ctgaatcacc tgaaggagag caaggatctg aagctgcaga
     5641 acggcatctc caatcaggac tggctggcct acatccagga gctgcgcaac aaaaggccgg
     5701 cggccacgaa aaaggccggc caggcaaaaa agaaaaaggg atcctaccca tacgatgttc
     5761 cagattacgc ttatccctac gacgtgcctg attatgcata cccatatgat gtccccgact
     5821 atgccggaag cgaggccagc ggttccggac gggctgacgc attggacgat tttgatctgg
     5881 atatgctggg aagtgacgcc ctcgatgatt ttgaccttga catgcttggt tcggatgccc
     5941 ttgatgactt tgacctcgac atgctcggca gtgacgccct tgatgatttc gacctggaca
     6001 tgctgattaa ctctagaagt tccggatctc cgaaaaagaa acgcaaagtt ggtagccagt
     6061 acctgcccga caccgacgac cggcaccgga tcgaggaaaa gcggaagcgg acctacgaga
     6121 cattcaagag catcatgaag aagtccccct tcagcggccc caccgaccct agacctccac
     6181 ctagaagaat cgccgtgccc agcagatcca gcgccagcgt gccaaaacct gccccccagc
     6241 cttacccctt caccagcagc ctgagcacca tcaactacga cgagttccct accatggtgt
     6301 tccccagcgg ccagatctct caggcctctg ctctggctcc agcccctcct caggtgctgc
     6361 ctcaggctcc tgctcctgca ccagctccag ccatggtgtc tgcactggct caggcaccag
     6421 cacccgtgcc tgtgctggct cctggacctc cacaggctgt ggctccacca gcccctaaac
     6481 ctacacaggc cggcgagggc acactgtctg aagctctgct gcagctgcag ttcgacgacg
     6541 aggatctggg agccctgctg ggaaacagca ccgatcctgc cgtgttcacc gacctggcca
     6601 gcgtggacaa cagcgagttc cagcagctgc tgaaccaggg catccctgtg gcccctcaca
     6661 ccaccgagcc catgctgatg gaataccccg aggccatcac ccggctcgtg acaggcgctc
     6721 agaggcctcc tgatccagct cctgcccctc tgggagcacc aggcctgcct aatggactgc
     6781 tgtctggcga cgaggacttc agctctatcg ccgatatgga tttctcagcc ttgctgggct
     6841 ctggcagcgg cagccgggat tccagggaag ggatgttttt gccgaagcct gaggccggct
     6901 ccgctattag tgacgtgttt gagggccgcg aggtgtgcca gccaaaacga atccggccat
     6961 ttcatcctcc aggaagtcca tgggccaacc gcccactccc cgccagcctc gcaccaacac
     7021 caaccggtcc agtacatgag ccagtcgggt cactgacccc ggcaccagtc cctcagccac
     7081 tggatccagc gcccgcagtg actcccgagg ccagtcacct gttggaggat cccgatgaag
     7141 agacgagcca ggctgtcaaa gcccttcggg agatggccga tactgtgatt ccccagaagg
     7201 aagaggctgc aatctgtggc caaatggacc tttcccatcc gcccccaagg ggccatctgg
     7261 atgagctgac aaccacactt gagtccatga ccgaggatct gaacctggac tcacccctga
     7321 ccccggaatt gaacgagatt ctggatacct tcctgaacga cgagtgcctc ttgcatgcca
     7381 tgcatatcag cacaggactg tccatcttcg acacatctct gttttaagcg gccgcactcc
     7441 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
     7501 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     7561 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     7621 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     7681 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     7741 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     7801 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     7861 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     7921 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct
     7981 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8041 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8101 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8161 ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     8221 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     8281 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     8341 tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa
     8401 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8461 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg
     8521 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8581 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8641 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8701 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8761 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8821 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8881 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8941 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     9001 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9061 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9121 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9181 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9241 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9301 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9361 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9421 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9481 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9541 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9601 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9661 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9721 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9781 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9841 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9901 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9961 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    10021 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    10081 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    10141 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10201 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10261 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10321 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10381 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10441 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
//
