LOCUS       sequence-207615-        3693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number plasmid containing Ple265 (PCP2 MiniPromoter) 
            insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, 
            Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, 
            Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM
  TITLE     Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye 
            and Brain; Including Seventeen Carrying a New Inducible-First 
            Constitutive-Ready Allele.
  JOURNAL   Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984.
  PUBMED    30765420
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984."
COMMENT     Sequence Label: pEMS1942
FEATURES             Location/Qualifiers
     source          1..3693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     622..641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(860..951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     993..1012
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1018..1037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(2696..2713)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2696..2712)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2811..2828)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2982..3570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3062..3081)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3692..3693,1..790,791..859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgccctttt gacgggcgcg cctggactcc agtcactttt
     1261 ctgctggcct ctgccccggc ccagtgccag agagggcctt ttaaacgtcc aggacgtggg
     1321 ggtgtggatt ccccccatcc ccaaatcccc agctcatgcc ccatctgctc ccacccaagc
     1381 ttaagcccca taaagatcat ccagataatt gtttgcccac aacgatgctg gatctggcat
     1441 ggtgggtagg gggcagggcg acctgagctt ggacctcgct gtgcccagca gcaatgactg
     1501 gaccccaggg actctcaggt gtgatgtcac aaaaggcctg agcagcgtcc ccacagttgg
     1561 agaagggagg ccaacagtgt agggtggtag ctggcttagg gaggacttgc aggtgtcccc
     1621 acccagccac ccaggttccc ccactggctc ctggaagggc agctgccacc tgcagtcggt
     1681 aaagaaaaag gaggttggag tcaaaggccc cttccctccc tccctctctg cacctgcctc
     1741 agacaccacc cgccacccta gctccagctc tgtctccttg gcagcaagtc aaggggtctc
     1801 ccagccaccc ttccttcaac tcactgtccc tggagcctgg ttgcctgccc cagaccccaa
     1861 aacaagaaag tctggggtct cctgttccca caccacctaa atgccaggtt gagctatctc
     1921 ctctcctcta tcaccctagc cccaggtggg aacaggaggc gagactccct ggggacagag
     1981 gcaggcgggg agaggatgtc cctgcctttt accccgagcc actgtggctt tccctcagtg
     2041 cccaagcagg ggataccact gggaggtccc catccccttc ccttccctcc ccaagacgtc
     2101 tttacggaaa ccagtggaag tttcatctca gaatgaatgg gctgcactat caccacctct
     2161 gcccgagcct cctctgttcc acaaagggcc aagttcacac tctctccctc tatgccaatg
     2221 aagtgtccac gcgtttcagc gaagggcgac acaaaattta ttctaaatgc ataataaata
     2281 ctgataacat cttatagttt gtattatatt ttgtattatc gttgacatgt ataattttga
     2341 tatcaaaaac tgattttccc tttattattt tcgagattta ttttcttaat tctctttaac
     2401 aaactagaaa tattgtatat acaaaaaatc ataaataata gatgaatagt ttaattatag
     2461 gtgttcatca atcgaaaaag caacgtatct tatttaaagt gcgttgcttt tttctcattt
     2521 ataaggttaa ataattctca tatatcaagc aaagtgacag gcgcccttaa atattctgac
     2581 aaatgctctt tccctaaact ccccccataa aaaaacccgc cgaagcgggt ttttacgtta
     2641 tttgcggatt aacgattact cgttatcaga accgcccagg gggcccgagc ttaagactgg
     2701 ccgtcgtttt acaacacaga aagagtttgt agaaacgcaa aaaggccatc cgtcaggggc
     2761 cttctgctta gtttgatgcc tggcagttcc ctactctcgc cttccgcttc ctcgctcact
     2821 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2881 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2941 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3001 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3061 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3121 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3181 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3241 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3301 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3361 aggtatgtag gcggtgctac agagttcttg aagtggtggg ctaactacgg ctacactaga
     3421 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3481 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3541 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3601 gacgctcagt ggaacgacgc gcgcgtaact cacgttaagg gattttggtc atgagcttgc
     3661 gccgtcccgt caagtcagcg taatgctctg ctt
//
