LOCUS       sequence-207618-        2998 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number plasmid containing Hspa1a (synonym Hsp68) 
            MiniPromoter insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, 
            Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, 
            Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM
  TITLE     Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye 
            and Brain; Including Seventeen Carrying a New Inducible-First 
            Constitutive-Ready Allele.
  JOURNAL   Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984.
  PUBMED    30765420
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984."
COMMENT     Sequence Label: pEMS1951
FEATURES             Location/Qualifiers
     source          1..2998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..22
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1068..1077
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1206..1222)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1206..1222)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      1270..1301
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1302..1404
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             join(1405..1473,1474..2265)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1623..1642)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      2337..2364
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2376..2963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2456..2475)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcga ccgccggagg gttgcgtttg agacgggcga cagatcgaca ctgctcgatc
      181 cgctcgcacc acgcgtgagc tccaggaaca tccaaactga gcagccgggg tcccccccac
      241 cccccacccc gccccacgcg gcaactttga gcctgtgctg ggacagagcc tctagttcct
      301 aaattagtcc atgaggtcag aggcagcact gccattgtaa cgcgattgga gaggatcacg
      361 tcaccggaca cgcccccagg catctccctg ggtctcctaa acttggcggg gagaagtttt
      421 agcccttaag ttttagcctt taacccccat attcagaact gtgcgagttg gcgaaacccc
      481 acaaatcaca acaaactgta cacaacaccg agctagaggt gatctttctt gtccattcca
      541 cacaggcctt agtaatgcgt cgccatagca acagtgtcac tagtagcacc agcacttccc
      601 cacaccctcc ccctcaggaa tccgtactct ccagtgaacc ccagaaacct ctggagagtt
      661 ctggacaagg gcggaaccca caactccgat tactcaaggg aggcggggaa gctccaccag
      721 acgcgaaact gctggaagat tcctggcccc aaggcctcct ccggctcgct gattggccca
      781 gcggagagtg ggcggggccg gtgaagactc cttaaaggcg cagggcggcg agcaggtcac
      841 cagacgctga cagctactca gaaccaaatc tggttccatc cagagacaag cgaagacaag
      901 agaagcagag cgagcggcgc gttcccgatc ctcggccagg accagccttc cccagagcat
      961 ccctgccgcg gagcgcaacc ttcccaggag catccctgcc gcggagcgca actttccccg
     1021 gagcatccac gccgcggagc gcagccttcc agaagcagag cgcggcgcca tggcggcgcg
     1081 ccggatcgac gagagcagcg cgactggatc agttctggac gagcgagctg tcgtccgacc
     1141 cgtgatctta cggcattata cgtatgatcg gtccacgatc agctagatta tctagtcagc
     1201 ttgatgtcat agctgtttcc tgaggctcaa tactgaccat ttaaatcata cctgacctcc
     1261 atagcagaaa gtcaaaagcc tccgaccgga ggcttttgac ttgatcggca cgtaagaggt
     1321 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
     1381 ttttcaggag ctaaggaagc taaaatgagt attcaacatt tccgtgtcgc ccttattccc
     1441 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1501 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1561 aagatccttg agagtttacg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1621 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     1681 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatctcacg
     1741 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1801 gccaacttac ttctggcaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     1861 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     1921 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     1981 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2041 aaagttgcag gatcacttct gcgctcggcc ctcccggctg gctggtttat tgctgataaa
     2101 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2161 ccctcccgca tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2221 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaatgagg gcccaaatgt
     2281 aatcacctgg ctcaccttcg ggtgggcctt tcttgaggac ctaaatgtaa tcacctggct
     2341 caccttcggg tgggcctttc tgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2401 gagcatcaca aaaatcgatg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2461 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2521 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2581 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2641 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2701 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2761 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     2821 gtatttggta tctgcgctct gctgaagcca gttacctcgg aaaaagagtt ggtagctctt
     2881 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2941 cgcgcagaaa aaaaggatct caagaagatc ctttgatttt ctaccgaaga aaggccca
//
