LOCUS       sequence-207623-        9217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression retroviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Lindsey JC, Kawauchi D, Schwalbe EC, Solecki DJ, Selby MP, McKinnon 
            PJ, Olson JM, Hayden JT, Grundy RG, Ellison DW, Williamson D, Bailey
            S, Roussel MF, Clifford SC
  TITLE     Cross-species epigenetics identifies a critical role for VAV1 in SHH
            subgroup medulloblastoma maintenance.
  JOURNAL   Oncogene. 2015 Sep 3;34(36):4746-57. doi: 10.1038/onc.2014.405. Epub
            2014 Dec 22.
  PUBMED    25531316
REFERENCE   2  (bases 1 to 9217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2014.405"; journalName: "Oncogene"; date: "2015-09-3- 
            3"; volume: "34"; issue: "36"; pages: "4746-57"
COMMENT     Sequence Label: MSCV mouse Vav1 IRES GFP
FEATURES             Location/Qualifiers
     source          1..9217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    419..760
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             827..1243
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1173..1195
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1211..1227
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1377..1386
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4017..4566
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4161..4178)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4388..4407
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4569..4571,4572..4574,4575..5288)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4569..4571,4572..4574,4575..5285)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4614..4635)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4875..4894)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5222..5243
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5450..5964
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6126..6142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6150..6156,6157..6174,6175..6180))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6195..6216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6333..6350)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6504..7092)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6584..6603)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7263..8054,8055..8123))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7886..7905
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8124..8228)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8296..8314
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8352..8374)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8474..8493
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8687..8709
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8837..8856
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9056..9217,1..355)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc
       61 tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat
      121 ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc
      181 aataaaagag cccacaaccc ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc
      241 gggtacccgt attcccaata aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga
      301 tccttgggag ggtctcctca gattgattga ctgcccacct cgggggtctt tcatttggag
      361 gttccaccga gatttggaga cccctgccca gggaccaccg acccccccgc cgggaggtaa
      421 gctggccagc ggtcgtttcg tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta
      481 atgtttgcgc ctgcgtctgt actagttagc taactagctc tgtatctggc ggacccgtgg
      541 tggaactgac gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg
      601 gggccgtttt tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg
      661 atatgtggtt ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt
      721 gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct
      781 gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac
      841 cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca
      901 gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt
      961 taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat
     1021 caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg
     1081 ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc
     1141 gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc
     1201 tcgatcctcc ctttatccag ccctcactcc ttctctaggc gccggaattg ggatcccagt
     1261 gtggtggtac gtaggaattc agatctcgac ccacgcgtcc gcggacgcgt gggcggcggt
     1321 ggtgaaggaa cgagggtgca cggggcctcc ccgaggggcc aagtgagagg ccggcagcca
     1381 ccatggagct ctggcgacag tgcacccact ggctgatcca gtgtcgggtg ctgcctccca
     1441 gccaccgtgt gacctgggag ggggcccagg tgtgtgagct ggcacaggca ctgcgggacg
     1501 gtgtcctctt gtgccaattg cttaacaacc tgcttcccca ggccattaat cttcgcgagg
     1561 ttaacttgcg gccccagatg tcccagttcc tttgtcttaa gaacattcga accttcctgt
     1621 ctacttgctg tgagaagttc ggcctcaagc gcagtgaact ctttgaggct tttgacctct
     1681 tcgatgtgca ggactttgga aaggtcatct acaccctgtc tgctctgtca tggacaccca
     1741 ttgcccagaa caaaggaatc atgcccttcc caacagagga cagcgctctg aacgacgaag
     1801 atatttacag tggcctttca gaccagattg atgacaccgc agaggaagac gaggaccttt
     1861 atgactgcgt ggaaaatgag gaggcagagg gggacgagat ctacgaggac ctaatgcgct
     1921 tggagtcggt gcctacgcca cccaagatga cagagtatga taagcgctgc tgctgcctgc
     1981 gggagatcca gcagacggag gagaagtata cagacacact gggctccatc cagcagcact
     2041 tcatgaagcc tctgcagcga ttccttaagc ctcaagacat ggagaccatc tttgtcaaca
     2101 ttgaggagct gttctctgtg catacccact tcttaaagga actgaaggat gccctggctg
     2161 gcccgggagc aacaacactg tatcaggtct tcatcaagta caaggagagg ttcctggttt
     2221 atggccgtta ttgcagtcag gtggagtcag ccagcaagca cttggatcaa gtggccacag
     2281 cacgggagga tgtgcagatg aagctggagg aatgttctca aagagctaac aatggccgat
     2341 tcaccctacg ggatctgctg atggtaccta tgcagcgggt gctgaagtac cacctccttc
     2401 tccaggagct agtgaaacac acacaggatg ctacagagaa ggagaacctg cggttggccc
     2461 tggacgccat gagggacctg gcacagtgcg tgaacgaggt caagagggac aatgaaaccc
     2521 tacggcagat cacaaacttt cagctgtcca ttgagaacct ggaccagtct ctggctaact
     2581 atggccggcc caagattgac ggtgagctca agattacctc agtggagcgt cgctcaaaga
     2641 cagacaggta tgccttcctg ctggacaaag cactgctcat ctgtaaacgc cgcggggact
     2701 cttacgacct caaagcctcg gtgaacttgc acagcttcca agttcgagat gactcctccg
     2761 gggagcgaga caacaagaag tggagccata tgttccttct gattgaggat caaggcgccc
     2821 agggctatga gctgttcttc aagactcggg agctgaagaa gaagtggatg gaacagttcg
     2881 aaatggccat ctccaacatt tacccagaga atgctacagc caatgggcat gattttcaga
     2941 tgttctcctt tgaggagacc acttcctgca aggcctgcca gatgttactc agaggcacat
     3001 tctaccaggg atatcgctgt tacaggtgcc gggcacctgc acacaaggag tgtctgggga
     3061 gagtgcctcc ctgtggtcgc catgggcaag atttcgcagg aaccatgaag aaggacaagc
     3121 tccatcgaag ggcccaggac aagaaaagga atgaattggg tctgcctaag atggaagtgt
     3181 ttcaggaata ctatgggatc cctcctccac ctggagcctt tgggccattt ttacggctca
     3241 accctgggga cattgtggag ctcactaagg cagaggctga gcacaactgg tgggagggaa
     3301 ggaatactgc tacaaatgaa gtcggctggt ttccctgtaa cagagtgcat ccctatgtcc
     3361 acggccctcc tcaggacctg tctgtgcatc tctggtatgc gggccctatg gaacgagcag
     3421 gcgctgaggg catcctcacc aaccgttctg atgggaccta tctggtgcgg cagagggtga
     3481 aagatacagc ggagttcgcc atcagcatta agtataacgt ggaggtcaag catattaaaa
     3541 tcatgacgtc agaggggttg taccggatca cagagaagaa ggctttccgg ggccttctgg
     3601 aactggtaga gttttatcag cagaattccc tcaaagattg cttcaagtcg ttggacacca
     3661 ccttgcagtt tccttataag gaacctgaga ggagagccat cagcaagcca ccagctggaa
     3721 gcaccaagta ttttggcact gccaaagccc gctacgactt ctgtgcccgg gacaggtcgg
     3781 aactgtccct taaggagggt gatatcatca agatcctcaa taagaaggga cagcaaggct
     3841 ggtggcgtgg ggagatctac ggccggatcg gctggttccc ttctaactat gtggaggaag
     3901 actattccga atattgctga gcctggtgcc ctgtaggaca cagagagagg cagatgaagg
     3961 ctgagcccag gatgctagag tatccctcga gcgggatcaa ttccgccccc cccctaacgt
     4021 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     4081 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     4141 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     4201 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     4261 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     4321 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     4381 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     4441 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     4501 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     4561 ataataccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     4621 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     4681 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     4741 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     4801 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     4861 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     4921 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     4981 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     5041 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     5101 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     5161 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     5221 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     5281 acaagtaaag cggccgcgac tctagagtcg acctgcaggc atgcaagctt caggtagccg
     5341 gctaacgtta acaaccggta cctctagaac tatagctagc atgcgcaaat ttaaagcgct
     5401 gatatcgata aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc
     5461 cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaataca
     5521 taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca gaatatgggc
     5581 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
     5641 cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
     5701 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
     5761 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc
     5821 gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt gtatccaata aaccctcttg
     5881 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
     5941 tacccgtcag cgggggtctt tcatgggtaa cagtttcttg aagttggaga acaacattct
     6001 gagggtagga gtcgaatatt aagtaatcct gactcaatta gccactgttt tgaatccaca
     6061 tactccaata ctcctgaaat agttcattat ggacagcgca gaaagagctg gggagaattg
     6121 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6181 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6241 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6301 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6361 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6421 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6481 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6541 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6601 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6661 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6721 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6781 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6841 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6901 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6961 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7021 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7081 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7141 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7201 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7261 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7321 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7381 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7441 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7501 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7561 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7621 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7681 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7741 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7801 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7861 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7921 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7981 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8041 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8101 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8161 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8221 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     8281 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     8341 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     8401 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     8461 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     8521 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
     8581 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     8641 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     8701 tgtaaaacga cggcgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
     8761 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     8821 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     8881 cggtgatgcc ggccacgatg cgtccggcgt agaggcgatt agtccaattt gttaaagaca
     8941 ggatatcagt ggtccaggct ctagttttga ctcaacaata tcaccagctg aagcctatag
     9001 agtacgagcc atagataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     9061 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
     9121 aaatacataa ctgagaatag agaagttcag atcaaggtta ggaacagaga gacagcagaa
     9181 tatgggccaa acaggatatc tgtggtaagc agttcct
//
