LOCUS       sequence-207632-       12370 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin
            resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12370)
  AUTHORS   Hodges HC, Stanton BZ, Cermakova K, Chang CY, Miller EL, Kirkland 
            JG, Ku WL, Veverka V, Zhao K, Crabtree GR
  TITLE     Dominant-negative SMARCA4 mutants alter the accessibility landscape 
            of tissue-unrestricted enhancers.
  JOURNAL   Nat Struct Mol Biol. 2018 Jan;25(1):61-72. doi: 
            10.1038/s41594-017-0007-3. Epub 2017 Dec 11.
  PUBMED    29323272
REFERENCE   2  (bases 1 to 12370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-017-0007-3"; journalName: "Nat Struct Mol Biol"; 
            date: "2018-01"; volume: "25"; issue: "1"; pages: "61-72"
COMMENT     Sequence Label: WT Smarca4-V5 IRES-puro
FEATURES             Location/Qualifiers
     source          1..12370
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4877..4918
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4997..5569
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5164..5181)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5391..5410
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5572..6174
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6071..6091
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6246..6834
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6299..6319)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6717..6728)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6921..7154
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7226..7347
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7263..7282)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7317..7336
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7387..7522
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7542..7561)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7543..7561)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7571..7588)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7571..7587)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7580..7602)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7729..8184
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7816..7835)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8026..8047
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8210..8314
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8315..8383,8384..9175)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8533..8552)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9346..9934
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9835..9854
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10088..10105
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10222..10243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10258..10263,10264..10281,10282..10288)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10296..10312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10301..10323
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10320..10336
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10320..10336
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10355..10375
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10357..10375
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10403..10629
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10630..10810
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10857..10982
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11475..11708
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11893..11937
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12204..12321
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccaccgggct agcatcgcca ccatgtctac tccagaccca cccttgggtg ggactcctcg
       61 gcctggtcct tccccaggcc ctggtccttc acctggtgca atgctgggtc ctagccctgg
      121 cccctcacca ggttctgccc acagcatgat ggggccaagc ccaggacctc cttcagcagg
      181 acatcccatg cccacccagg ggcctggagg gtacccccag gacaacatgc atcagatgca
      241 caagcctatg gagtccatgc acgagaaggg catgcctgat gacccacgat acaaccagat
      301 gaaagggatg ggcatgcggt caggggccca cacaggcatg gcacctccac ctagtcccat
      361 ggaccagcat tctcaaggtt acccctcacc cctcggcggc tctgaacatg cctccagtcc
      421 tgtcccagcc agtggcccat cttcaggccc ccagatgtcc tctgggccag gaggggcccc
      481 actagatggt tctgatcccc aggccttggg acagcaaaac agaggcccaa ccccatttaa
      541 ccagaaccag ctgcatcaac tcagagctca gataatggcc tacaagatgt tggccagggg
      601 ccagccattg cccgaccacc tgcagatggc cgtgcaaggc aagcggccga tgcctggaat
      661 gcagcaacag atgccaacac tacctccacc ctcagtgtcc gccacaggac ccggacctgg
      721 acccggccct ggccctggcc ctggcccagg accagcccct ccaaattaca gtagacccca
      781 tggtatggga gggcccaaca tgcctccccc aggaccctca ggtgtgcccc ccgggatgcc
      841 tggtcagccg cctggagggc ctcccaagcc atggcctgaa ggacccatgg ccaatgctgc
      901 tgcccccaca agcaccccac agaagctgat tcctccgcaa ccaacaggcc gtccttcacc
      961 tgcacctcct gctgtcccgc ctgctgcctc acctgtaatg ccaccacaaa cacagtcccc
     1021 agggcagcca gcccagcctg ctccattggt gccactgcac cagaagcaga gccgaatcac
     1081 ccccatccag aagccccgag gccttgaccc tgtggagatc ctacaagagc gggagtacag
     1141 gcttcaggct cgaatcgcac acagaattca ggaacttgaa aacctccctg ggtccctggc
     1201 tggggacctt cgaaccaaag caaccatcga actcaaggcc cttaggttgc tgaacttcca
     1261 gaggcagctg cgccaggagg tggtggtgtg catgcgaaga gacacagccc tggagacagc
     1321 cctcaatgcc aaggcctaca agcgcagcaa acgtcagtca ctacgggagg cccgcatcac
     1381 tgagaagttg gagaagcagc agaagattga acaggagcgc aagcgccgcc agaagcacca
     1441 ggagtacctc aacagcattc tgcagcatgc caaggacttc agggagtatc acagatcagt
     1501 cacaggcaaa ctccagaaac tcaccaaggc tgtggccacc taccatgcca acactgagcg
     1561 ggagcagaag aaagaaaatg agcgcattga gaaggagcga atgcggaggc ttatggctga
     1621 agatgaggag ggctaccgca aactcattga ccagaagaag gacaagcgcc tggcctacct
     1681 tctgcagcag acagatgagt atgtggccaa cctcacagag ctggtgcggc agcacaaagc
     1741 tgcccaggtt gccaaggaga agaagaagaa aaagaaaaag aagaaggcag aaaatgctga
     1801 aggacagaca cctgctattg gaccagatgg tgagcctctg gatgagacca gccagatgag
     1861 tgacctccct gtgaaggtga tccacgtgga gagtggcaag atcctcactg gcacagatgc
     1921 cccaaaagcc gggcagctgg aagcctggct tgaaatgaac ccagggtatg aagtagcccc
     1981 caggtcagac agtgaagaaa gtggctctga agaggaggag gaggaggagg aagaggagca
     2041 gcctcagccc gcacagcccc ctacactgcc tgtggaagaa aagaagaaga ttccagaccc
     2101 agacagcgat gatgtctctg aggtggacgc ccgacacatt attgagaacg ccaagcaaga
     2161 tgtggacgat gagtacggtg tgtcccaggc ccttgctcgt ggcctgcagt cttactatgc
     2221 tgtggcccat gcagtcacag agagagtaga taagcagtcc gccctcatgg tcaacggtgt
     2281 cctcaaacag taccagatca agggtttgga gtggctggtg tccctgtaca acaacaacct
     2341 gaatggcatc ctggctgatg agatggggct ggggaagacc atccagacca tcgcgctcat
     2401 cacatacctc atggagcaca agcgcatcaa cgggcctttc ctcatcatcg tgcctctctc
     2461 gacactgtca aactgggcgt atgaatttga caagtgggcc ccctctgtgg tgaaggtttc
     2521 ttacaagggc tctccagctg caaggcgagc ttttgtccca cagcttcgca gtgggaagtt
     2581 caacgtctta ctgaccacct atgaatatat catcaaagac aagcatatcc tagccaagat
     2641 ccgctggaag tacatgattg tggatgaagg ccaccgcatg aaaaaccacc actgcaagtt
     2701 gacgcaggtc cttaacacac actacgtggc ccctcggcgc ctgcttctta caggcacacc
     2761 actgcagaac aagctaccgg agctctgggc cctgcttaac ttcctgctcc ccactatctt
     2821 caagagctgc agcaccttcg aacagtggtt caatgcaccc tttgccatga ctggagaaaa
     2881 ggtggacctg aatgaagagg agactatcct cattattcgt cgcctacaca aagttctgcg
     2941 gcccttcctg ctgcggcggc tcaagaagga agttgaagcc cagctccctg agaaggtaga
     3001 gtatgtcatc aaatgcgaca tgtcagccct gcagcgtgtg ctgtaccgtc acatgcaggc
     3061 caaaggtgtg ctgctgactg acggctccga gaaggacaag aagggcaaag gtggcaccaa
     3121 gacactgatg aacactatta tgcaactgcg taagatctgc aaccacccct acatgttcca
     3181 gcacatcgag gagtcctttt ctgagcactt ggggttcacc ggcggcatcg tgcaaggatt
     3241 ggacctttac cgtgcctcag ggaaatttga acttcttgat agaattctac ccaaactccg
     3301 tgcaacgaac cataaagtgc tcctcttttg ccaaatgacc tccctcatga ccatcatgga
     3361 agactacttt gcataccgtg gcttcaaata cctcaggctt gatggaacca caaaagcaga
     3421 agaccggggc atgctgttga aaacctttaa tgaacctggc tctgagtatt tcattttcct
     3481 gctcagtacc cgtgctgggg ggctgggcct gaatctgcag tcagctgaca ctgtgatcat
     3541 ctttgacagt gactggaatc cccaccagga cctacaagca caggatcgag cccatcgcat
     3601 tggacagcag aatgaggtgc gtgttcttcg cctgtgcacg gtcaacagtg tggaagagaa
     3661 gatactggct gctgccaaat acaaactcaa tgtggatcag aaggtgatcc aggcaggcat
     3721 gttcgaccag aagtcgtcca gccatgagag gcgtgccttc ctgcaggcca tcctggagca
     3781 cgaggagcag gatgaggagg aagatgaggt gcctgatgat gagaccgtca accagatgat
     3841 tgcccggcac gaagaagagt ttgacctctt catgcgcatg gacttggacc gccggcgtga
     3901 agaagcccgc aaccccaagc ggaagccacg cctgatggaa gaggatgagc tcccatcctg
     3961 gatcatcaag gatgatgccg aggtggagcg gctgacatgt gaagaggaag aggagaagat
     4021 gttcggccgt ggttctcgcc accgcaagga ggtagactac agcgactcac tgacagagaa
     4081 gcagtggctc aaggctatcg aggagggcac gctggaggag atcgaagagg aggtccggca
     4141 gaagaaatct tcacgtaagc gtaagcgaga cagcgaggcc ggctcctcca ccccgaccac
     4201 cagcacccgc agccgtgaca aggatgagga gagcaagaag cagaagaaac gtgggcggcc
     4261 acctgctgag aagctgtccc caaacccacc taacctcacc aagaagatga agaagatcgt
     4321 ggatgctgtg atcaagtaca aagacagcag cagtggacgt cagctcagcg aggtgttcat
     4381 ccagctcccc tctcgcaagg agcttcctga gtactatgag ctcatccgaa agcctgtgga
     4441 cttcaagaag atcaaggaac gcatccgaaa ccacaagtac cgcagcctca atgacctgga
     4501 gaaggatgtg atgctgctgt gccagaacgc tcagacgttc aacctcgagg gttccctgat
     4561 ctatgaggac tccatcgtcc tgcagtctgt cttcaccagc gtacggcaga agattgagaa
     4621 ggaggacgac agtgaaggcg aggaaagcga ggaggaggag gagggcgagg aggaaggctc
     4681 cgagtctgag tcccgctccg tcaaggtgaa gatcaagctg ggccgcaagg agaaggccca
     4741 ggaccgactc aaggggggcc gccggcggcc aagccgggga tcccgggcca agccggttgt
     4801 gagtgacgat gacagtgagg aggagcagga ggaggaccgc tcaggaagtg gcagtgagga
     4861 agacggtgga accggtggta agcctatccc taaccctctc ctcggtctcg attctacgta
     4921 acgcgtccgg tagtggagga tccgtcgacg cagatgccgg tgaagaataa tgtacaagta
     4981 gcggccgcaa attccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     5041 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     5101 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     5161 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     5221 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     5281 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     5341 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     5401 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     5461 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     5521 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgataatac catggccacc
     5581 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc
     5641 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac
     5701 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     5761 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     5821 gaagcggggg cggtgttcgc cgagatcggc tcgcgcatgg ccgagttgag cggttcccgg
     5881 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     5941 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6001 gtcgtgctcc ccggagtgga ggcggccgag cgcgctgggg tgcccgcctt cctggagacc
     6061 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6121 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagtttgt
     6181 ttgaatgagg cttcagtact ttacagaatc gttgcctgca cgatatcgta caagtaagat
     6241 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6301 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6361 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6421 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6481 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6541 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6601 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     6661 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6721 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6781 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     6841 ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6901 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     6961 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7021 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7081 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7141 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     7201 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     7261 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7321 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     7381 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7441 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     7501 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     7561 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     7621 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     7681 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     7741 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     7801 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     7861 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     7921 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     7981 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     8041 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     8101 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     8161 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     8221 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     8281 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     8341 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     8401 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     8461 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     8521 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     8581 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     8641 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     8701 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     8761 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     8821 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     8881 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     8941 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     9001 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     9061 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     9121 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     9181 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     9241 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     9301 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     9361 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     9421 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     9481 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     9541 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9601 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9661 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9721 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9781 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9841 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9901 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9961 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    10021 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    10081 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
    10141 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
    10201 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
    10261 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
    10321 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
    10381 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
    10441 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
    10501 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
    10561 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
    10621 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
    10681 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
    10741 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
    10801 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
    10861 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
    10921 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
    10981 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
    11041 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
    11101 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
    11161 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
    11221 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
    11281 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
    11341 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
    11401 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
    11461 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
    11521 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
    11581 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
    11641 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
    11701 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
    11761 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
    11821 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
    11881 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    11941 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    12001 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
    12061 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
    12121 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
    12181 ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca
    12241 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
    12301 attacaaaaa ttcaaaattt tatcgatcac gagactagcc tcgagaagct tgatcggcca
    12361 cgcggatctc
//
