LOCUS       sequence-207635-       12419 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with 
            IRES puromycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12419)
  AUTHORS   Hodges HC, Stanton BZ, Cermakova K, Chang CY, Miller EL, Kirkland 
            JG, Ku WL, Veverka V, Zhao K, Crabtree GR
  TITLE     Dominant-negative SMARCA4 mutants alter the accessibility landscape 
            of tissue-unrestricted enhancers.
  JOURNAL   Nat Struct Mol Biol. 2018 Jan;25(1):61-72. doi: 
            10.1038/s41594-017-0007-3. Epub 2017 Dec 11.
  PUBMED    29323272
REFERENCE   2  (bases 1 to 12419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-017-0007-3"; journalName: "Nat Struct Mol Biol"; 
            date: "2018-01"; volume: "25"; issue: "1"; pages: "61-72"
COMMENT     Sequence Label: K785R Smarca4-V5 IRES-puro
FEATURES             Location/Qualifiers
     source          1..12419
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4926..4967
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5046..5618
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5213..5230)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5440..5459
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5621..6223
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6120..6140
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6295..6883
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6348..6368)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6766..6777)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6970..7203
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7275..7396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7312..7331)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7366..7385
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7436..7571
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7591..7610)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7592..7610)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7620..7637)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7620..7636)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7629..7651)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7778..8233
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7865..7884)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8075..8096
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8259..8363
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8364..8432,8433..9224)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8582..8601)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9395..9983
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9884..9903
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10137..10154
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10271..10292
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10307..10312,10313..10330,10331..10337)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10345..10361
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10350..10372
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10369..10385
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10369..10385
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10404..10424
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10406..10424
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10452..10678
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10679..10859
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10906..11031
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11524..11757
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11942..11986
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12253..12370
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc caccgggcta
       61 gcatcgccac catgtctact ccagacccac ccttgggtgg gactcctcgg cctggtcctt
      121 ccccaggccc tggtccttca cctggtgcaa tgctgggtcc tagccctggc ccctcaccag
      181 gttctgccca cagcatgatg gggccaagcc caggacctcc ttcagcagga catcccatgc
      241 ccacccaggg gcctggaggg tacccccagg acaacatgca tcagatgcac aagcctatgg
      301 agtccatgca cgagaagggc atgcctgatg acccacgata caaccagatg aaagggatgg
      361 gcatgcggtc aggggcccac acaggcatgg cacctccacc tagtcccatg gaccagcatt
      421 ctcaaggtta cccctcaccc ctcggcggct ctgaacatgc ctccagtcct gtcccagcca
      481 gtggcccatc ttcaggcccc cagatgtcct ctgggccagg aggggcccca ctagatggtt
      541 ctgatcccca ggccttggga cagcaaaaca gaggcccaac cccatttaac cagaaccagc
      601 tgcatcaact cagagctcag ataatggcct acaagatgtt ggccaggggc cagccattgc
      661 ccgaccacct gcagatggcc gtgcaaggca agcggccgat gcctggaatg cagcaacaga
      721 tgccaacact acctccaccc tcagtgtccg ccacaggacc cggacctgga cccggccctg
      781 gccctggccc tggcccagga ccagcccctc caaattacag tagaccccat ggtatgggag
      841 ggcccaacat gcctccccca ggaccctcag gtgtgccccc cgggatgcct ggtcagccgc
      901 ctggagggcc tcccaagcca tggcctgaag gacccatggc caatgctgct gcccccacaa
      961 gcaccccaca gaagctgatt cctccgcaac caacaggccg tccttcacct gcacctcctg
     1021 ctgtcccgcc tgctgcctca cctgtaatgc caccacaaac acagtcccca gggcagccag
     1081 cccagcctgc tccattggtg ccactgcacc agaagcagag ccgaatcacc cccatccaga
     1141 agccccgagg ccttgaccct gtggagatcc tacaagagcg ggagtacagg cttcaggctc
     1201 gaatcgcaca cagaattcag gaacttgaaa acctccctgg gtccctggct ggggaccttc
     1261 gaaccaaagc aaccatcgaa ctcaaggccc ttaggttgct gaacttccag aggcagctgc
     1321 gccaggaggt ggtggtgtgc atgcgaagag acacagccct ggagacagcc ctcaatgcca
     1381 aggcctacaa gcgcagcaaa cgtcagtcac tacgggaggc ccgcatcact gagaagttgg
     1441 agaagcagca gaagattgaa caggagcgca agcgccgcca gaagcaccag gagtacctca
     1501 acagcattct gcagcatgcc aaggacttca gggagtatca cagatcagtc acaggcaaac
     1561 tccagaaact caccaaggct gtggccacct accatgccaa cactgagcgg gagcagaaga
     1621 aagaaaatga gcgcattgag aaggagcgaa tgcggaggct tatggctgaa gatgaggagg
     1681 gctaccgcaa actcattgac cagaagaagg acaagcgcct ggcctacctt ctgcagcaga
     1741 cagatgagta tgtggccaac ctcacagagc tggtgcggca gcacaaagct gcccaggttg
     1801 ccaaggagaa gaagaagaaa aagaaaaaga agaaggcaga aaatgctgaa ggacagacac
     1861 ctgctattgg accagatggt gagcctctgg atgagaccag ccagatgagt gacctccctg
     1921 tgaaggtgat ccacgtggag agtggcaaga tcctcactgg cacagatgcc ccaaaagccg
     1981 ggcagctgga agcctggctt gaaatgaacc cagggtatga agtagccccc aggtcagaca
     2041 gtgaagaaag tggctctgaa gaggaggagg aggaggagga agaggagcag cctcagcccg
     2101 cacagccccc tacactgcct gtggaagaaa agaagaagat tccagaccca gacagcgatg
     2161 atgtctctga ggtggacgcc cgacacatta ttgagaacgc caagcaagat gtggacgatg
     2221 agtacggtgt gtcccaggcc cttgctcgtg gcctgcagtc ttactatgct gtggcccatg
     2281 cagtcacaga gagagtagat aagcagtccg ccctcatggt caacggtgtc ctcaaacagt
     2341 accagatcaa gggtttggag tggctggtgt ccctgtacaa caacaacctg aatggcatcc
     2401 tggctgatga gatggggctg gggaggacca tccagaccat cgcgctcatc acatacctca
     2461 tggagcacaa gcgcatcaac gggcctttcc tcatcatcgt gcctctctcg acactgtcaa
     2521 actgggcgta tgaatttgac aagtgggccc cctctgtggt gaaggtttct tacaagggct
     2581 ctccagctgc aaggcgagct tttgtcccac agcttcgcag tgggaagttc aacgtcttac
     2641 tgaccaccta tgaatatatc atcaaagaca agcatatcct agccaagatc cgctggaagt
     2701 acatgattgt ggatgaaggc caccgcatga aaaaccacca ctgcaagttg acgcaggtcc
     2761 ttaacacaca ctacgtggcc cctcggcgcc tgcttcttac aggcacacca ctgcagaaca
     2821 agctaccgga gctctgggcc ctgcttaact tcctgctccc cactatcttc aagagctgca
     2881 gcaccttcga acagtggttc aatgcaccct ttgccatgac tggagaaaag gtggacctga
     2941 atgaagagga gactatcctc attattcgtc gcctacacaa agttctgcgg cccttcctgc
     3001 tgcggcggct caagaaggaa gttgaagccc agctccctga gaaggtagag tatgtcatca
     3061 aatgcgacat gtcagccctg cagcgtgtgc tgtaccgtca catgcaggcc aaaggtgtgc
     3121 tgctgactga cggctccgag aaggacaaga agggcaaagg tggcaccaag acactgatga
     3181 acactattat gcaactgcgt aagatctgca accaccccta catgttccag cacatcgagg
     3241 agtccttttc tgagcacttg gggttcaccg gcggcatcgt gcaaggattg gacctttacc
     3301 gtgcctcagg gaaatttgaa cttcttgata gaattctacc caaactccgt gcaacgaacc
     3361 ataaagtgct cctcttttgc caaatgacct ccctcatgac catcatggaa gactactttg
     3421 cataccgtgg cttcaaatac ctcaggcttg atggaaccac aaaagcagaa gaccggggca
     3481 tgctgttgaa aacctttaat gaacctggct ctgagtattt cattttcctg ctcagtaccc
     3541 gtgctggggg gctgggcctg aatctgcagt cagctgacac tgtgatcatc tttgacagtg
     3601 actggaatcc ccaccaggac ctacaagcac aggatcgagc ccatcgcatt ggacagcaga
     3661 atgaggtgcg tgttcttcgc ctgtgcacgg tcaacagtgt ggaagagaag atactggctg
     3721 ctgccaaata caaactcaat gtggatcaga aggtgatcca ggcaggcatg ttcgaccaga
     3781 agtcgtccag ccatgagagg cgtgccttcc tgcaggccat cctggagcac gaggagcagg
     3841 atgaggagga agatgaggtg cctgatgatg agaccgtcaa ccagatgatt gcccggcacg
     3901 aagaagagtt tgacctcttc atgcgcatgg acttggaccg ccggcgtgaa gaagcccgca
     3961 accccaagcg gaagccacgc ctgatggaag aggatgagct cccatcctgg atcatcaagg
     4021 atgatgccga ggtggagcgg ctgacatgtg aagaggaaga ggagaagatg ttcggccgtg
     4081 gttctcgcca ccgcaaggag gtagactaca gcgactcact gacagagaag cagtggctca
     4141 aggctatcga ggagggcacg ctggaggaga tcgaagagga ggtccggcag aagaaatctt
     4201 cacgtaagcg taagcgagac agcgaggccg gctcctccac cccgaccacc agcacccgca
     4261 gccgtgacaa ggatgaggag agcaagaagc agaagaaacg tgggcggcca cctgctgaga
     4321 agctgtcccc aaacccacct aacctcacca agaagatgaa gaagatcgtg gatgctgtga
     4381 tcaagtacaa agacagcagc agtggacgtc agctcagcga ggtgttcatc cagctcccct
     4441 ctcgcaagga gcttcctgag tactatgagc tcatccgaaa gcctgtggac ttcaagaaga
     4501 tcaaggaacg catccgaaac cacaagtacc gcagcctcaa tgacctggag aaggatgtga
     4561 tgctgctgtg ccagaacgct cagacgttca acctcgaggg ttccctgatc tatgaggact
     4621 ccatcgtcct gcagtctgtc ttcaccagcg tacggcagaa gattgagaag gaggacgaca
     4681 gtgaaggcga ggaaagcgag gaggaggagg agggcgagga ggaaggctcc gagtctgagt
     4741 cccgctccgt caaggtgaag atcaagctgg gccgcaagga gaaggcccag gaccgactca
     4801 aggggggccg ccggcggcca agccggggat cccgggccaa gccggttgtg agtgacgatg
     4861 acagtgagga ggagcaggag gaggaccgct caggaagtgg cagtgaggaa gacggtggaa
     4921 ccggtggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtaa cgcgtccggt
     4981 agtggaggat ccgtcgacgc agatgccggt gaagaataat gtacaagtag cggccgcaaa
     5041 ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     5101 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     5161 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     5221 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     5281 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     5341 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     5401 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     5461 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     5521 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     5581 cacggggacg tggttttcct ttgaaaaaca cgataatacc atggccaccg agtacaagcc
     5641 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
     5701 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
     5761 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     5821 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     5881 ggtgttcgcc gagatcggct cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     5941 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     6001 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6061 cggagtggag gcggccgagc gcgctggggt gcccgccttc ctggagacct ccgcgccccg
     6121 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     6181 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtttgtt tgaatgaggc
     6241 ttcagtactt tacagaatcg ttgcctgcac gatatcgtac aagtaagatt cgacaatcaa
     6301 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6361 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6421 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6481 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6541 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6601 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6661 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     6721 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6781 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6841 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tcgagctcgg
     6901 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6961 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     7021 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7081 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7141 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7201 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7261 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7321 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7381 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     7441 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7501 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7561 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     7621 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     7681 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     7741 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     7801 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7861 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7921 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7981 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8041 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8101 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     8161 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     8221 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8281 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     8341 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     8401 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     8461 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     8521 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     8581 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     8641 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     8701 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     8761 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     8821 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     8881 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     8941 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     9001 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     9061 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     9121 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     9181 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     9241 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     9301 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9361 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9421 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9481 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9541 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     9601 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     9661 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     9721 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     9781 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     9841 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     9901 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     9961 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10021 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    10081 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    10141 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    10201 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    10261 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    10321 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    10381 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
    10441 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
    10501 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
    10561 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
    10621 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
    10681 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    10741 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    10801 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    10861 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
    10921 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
    10981 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
    11041 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
    11101 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
    11161 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
    11221 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
    11281 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
    11341 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
    11401 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
    11461 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
    11521 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
    11581 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
    11641 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
    11701 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
    11761 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    11821 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    11881 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
    11941 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    12001 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
    12061 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
    12121 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
    12181 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
    12241 gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
    12301 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
    12361 tcaaaatttt atcgatcacg agactagcct cgagaagctt gatcggccac gcggatctc
//
