LOCUS       sequence-207645-       10873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of ilux for bioluminescence imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10873)
  AUTHORS   Gregor C, Gwosch KC, Sahl SJ, Hell SW
  TITLE     Strongly enhanced bacterial bioluminescence with the ilux operon for
            single-cell imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 
            10.1073/pnas.1715946115. Epub 2018 Jan 16.
  PUBMED    29339494
REFERENCE   2  (bases 1 to 10873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1715946115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-01-30- 30"; volume: "115"; issue: "5"; pages: "962-967"
COMMENT     Sequence Label: ilux pGEX(-)
FEATURES             Location/Qualifiers
     source          1..10873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(44..49,50..65,66..72)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    80..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             119..1561
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIITGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHDIVNFLYTVGQRWKNDEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGPKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWTIIESDAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             1574..2497
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2552..3634
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMERLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGADMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESAATTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             3649..4632
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINPTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPAEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNEDFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             4817..5929
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     primer_bind     complement(6784..6806)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6951..6969)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7037..7141
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7142..7210,7211..8002)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7360..7379)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8173..8761
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8662..8681
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8915..8932
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        9005..9082
                     /gene="lacI"
                     /label=lacI promoter
     CDS             9083..10165
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(9102..9121)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(10214..10219,10220..10237,10238..10244)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10252..10268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10276..10292
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10276..10292
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             10288..10815
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(10308..10325)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10308..10324)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10317..10339)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa ttaatcatcg
       61 gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa caggatccat
      121 gactaaaaaa atttcattca ttattaccgg ccaggttgaa atctttcccg aaagcgatga
      181 tttagtgcaa tccattaatt ttggtgataa tagtgtttac ctgccaatat tgaatgactc
      241 tcatgtaaaa aacattattg attgtaatgg aaataacgaa ttacggttgc atgacattgt
      301 caattttctc tatacggtag ggcaaagatg gaaaaatgat gaatactcaa gacgcaggac
      361 atacattcgt gacttaaaaa aatatatggg atattcagaa gaaatggcta agctagaggc
      421 caattggata tctatgatat tatgttctaa aggcggcctt tatgatgttg tagaaaatga
      481 acttggttct cgccatatca tggatgaatg gctacctcag gatgaaagtt atgttcgggc
      541 ttttccgaaa ggtaaatctg tacatctgtt ggcaggtaat gttccattat ctgggatcat
      601 gtctatatta cgcgcaattt taactaagaa tcagtgtatt ataaaaacat cgtcaaccga
      661 tccttttacc gctaatgcat tagcgttaag ttttattgat gtagacccta atcatccgat
      721 aacgcgctct ttatctgtta tatattggcc ccaccaaggt gatacatcac tcgcaaaaga
      781 aattatgcga catgcggatg ttattgtcgc ttggggaggg ccagatgcga ttaattgggc
      841 ggtagagcat gcgccatctt atgctgatgt gattaaattt ggtcctaaaa agagtctttg
      901 cattatcgat aatcctgttg atttgacgtc tgcagcgaca ggtgcggctc atgatgtttg
      961 tttttacgat cagcgagctt gtttttctgc ccaaaacata tattatatgg gaaatcatta
     1021 tgaggaattt aagttagcgt tgatagaaaa acttaatcta tatgcgcata tattaccgaa
     1081 tgcaaaaaaa gattttgatg aaaaggcggc ctattcttta gttcaaaaag aaagcttgtt
     1141 tgctggatta aaagtagagg tggatattca tcaacgttgg acgattattg agtcagatgc
     1201 aggtgtggaa tttaatcaac cacttggcag atgtgtgtac cttcatcacg tcgataatat
     1261 tgagcaaata ttgccttatg ttcaaaaaaa taagacgcaa accatatcta tttttccttg
     1321 ggagtcatca tttaaatatc gagatgcgtt agcattaaaa ggtgcggaaa ggattgtaga
     1381 agcaggaatg aataacatat ttcgagttgg tggatctcat gacggaatga gaccgttgca
     1441 acgattagtg acatatattt ctcatgaaag gccatctaac tatacggcta aggatgttgc
     1501 ggttgaaata gaacagactc gtttcctgga agaagataag ttccttgtat ttgtcccata
     1561 ataggtaaaa agtatggaaa atgaatcaaa atataaaacc atcgaccacg ttatttgtgt
     1621 tgaaggaaat aaaaaaattc atgtttggga aacgctgcca gaagaaaaca gcccaaagag
     1681 aaagaatgcc attattattg cgtctggttt tgcccgcagg atggatcatt ttgctggtct
     1741 ggcggaatat ttatcgcgga atggatttca tgtgatccgc tatgattcgc ttcaccacgt
     1801 tggattgagt tcagggacaa ttgatgaatt tacaatgtct ataggaaagc agagcttgtt
     1861 agcagtggtt gattggttaa ctacacgaaa aataaataac ttcggtatgt tggcttcaag
     1921 cttatctgcg cggatagctt atgcaagcct atctgaaatc aatgcttcgt ttttaatcac
     1981 cgcagtcggt gttgttaact taagatattc tcttgaaaga gctttagggt ttgattatct
     2041 cagtctaccc attaatgaat tgccgaataa tctagatttt gaaggccata aattgggtgc
     2101 tgaagtcttt gcgagagatt gtcttgattt tggttgggaa gatttagctt ctacaattaa
     2161 taacatgatg tatcttgata taccgtttat tgcttttact gcaaataacg ataattgggt
     2221 caagcaagat gaagttatca cattgttatc aaatattcgt agtaatcgat gcaagatata
     2281 ttctttgtta ggaagttcgc atgacttgag tgaaaattta gtggtcctgc gcaattttta
     2341 tcaatcggtt acgaaagccg ctatcgcgat ggataatgat catctggata ttgatgttga
     2401 tattactgaa ccgtcatttg aacatttaac tattgcgaca gtcaatgaac gccgaatgag
     2461 aattgagatt gaaaatcaag caatttctct gtcttaaaat ctattgagat attctatcac
     2521 tcaaatagca atataaggac tctctgaatt catgaaattt ggaaactttt tacttacata
     2581 ccaacctccc caattttctc aaacagaggt aatggaacgt ttggttaaat taggtcgcat
     2641 ctctgaggag tgtggttttg ataccgtatg gttactggag catcatttca cggagtttgg
     2701 tctacttggt aacccttatg tcgctgctgc atatttactt ggcgcgacta aaaaattgaa
     2761 tgtaggaacc gccgctattg ttcttcccac agcccatcca gtacgccaac ttgaagatgt
     2821 gaatttattg gatcaaatgt caaaaggacg atttcggttt ggtatttgcc gagggcttta
     2881 caacaaggac tttcgcgtat tcggcgcgga tatgaataac agtcgcgcct tagcggaatg
     2941 ctggtacggg ctgataaaga atggcatgac agagggatat atggaagctg ataatgaaca
     3001 tatcaagttc cataaggtaa aagtaaaccc cgcggcgtat agcagaggtg gcgcaccggt
     3061 ttatgtggtg gctgaatcag ctgcgacgac tgagtgggca gctcaatttg gcctaccgat
     3121 gatattaagt tggattataa atactaacga aaagaaagca caacttgagc tttataatga
     3181 ggtggctcaa gaatatgggc acgatattca taatatcgac cattgcttat catatataac
     3241 atctgtagat catgactcaa ttaaagcgaa agagatttgc cggaaatttc tggggcattg
     3301 gtatgattct tatgtgaatg ctacgactat ttttgatgat tcagaccaaa caagaggtta
     3361 tgatttcaat aaagggcagt ggcgtgactt tgtattaaaa ggacataaag atactaatcg
     3421 ccgtattgat tacagttacg aaatcaatcc cgtgggaacg ccgcaggaat gtattgacat
     3481 aattcaaaaa gacattgatg ctacaggaat atcaaatatt tgttgtggat ttgaagctaa
     3541 tggaacagta gacgaaatta ttgcttccat gaagctcttc cagtctgatg tcatgccatt
     3601 tcttaaagaa aaacaacgtt cgctattata ttagctaagg agaaagaaat gaaatttgga
     3661 ttgttcttcc ttaacttcat caatccgaca actgttcaag aacaaagtat agttcgcatg
     3721 caggaaataa cggagtatgt tgataagttg aattttgaac agattttagt gtatgaaaat
     3781 catttttcag ataatggtgt tgtcggcgct cctctgactg tttctggttt tctgctcggt
     3841 ttaacagaga aaattaaaat tggttcatta aatcacatca ttacaactca tcatcctgtc
     3901 cgcatagcgg aggaagcttg cttattggat cagttaagtg aagggagatt tattttaggg
     3961 tttagtgatt gcgaaaaaaa agatgaaatg cattttttta atcgcccggc tgaatatcaa
     4021 cagcaactat ttgaagagtg ttatgaaatc attaacgatg ctttaacaac aggctattgt
     4081 aatccagata acgattttta tagcttccct aaaatatctg taaatcccca tgcttatacg
     4141 ccaggcggac ctcggaaata tgtaacagca accagtcatc atattgttga gtgggcggcc
     4201 aaaaaaggta ttcctctcat ctttaagtgg gatgattcta atgatgttag atatgaatat
     4261 gctgaaagat ataaagccgt cgcggataaa tatgacgttg acctatcaga gatagaccat
     4321 cagttaatga tattagttaa ctataacgaa gatagtaata aagctaaaca agagacgcgt
     4381 gcatttatta gtgattatgt tcttgaaatg caccctaatg aagatttcga aaataaactt
     4441 gaagaaataa ttgcagaaaa cgctgtcgga aattatacgg agtgtataac tgcggctaaa
     4501 ttggcaattg aaaagtgtgg tgcgaaaagt gtattgctgt cctttgaacc aatgaatgat
     4561 ttgatgagcc aaaaaaatgt aatcaatatt gttgatgata atattaagaa gtaccacatg
     4621 gaatatacct aaccatggta gatttcgagt tgcagcgagg cggcaagtga acgaatcccc
     4681 aggagcatag ataactatgt gactggggtg agtgaaagca gccaacaaag cagcagcttg
     4741 aaagatgaag ggtataaaag agtatgacag cagtgctgcc atactttcta atattatctt
     4801 gaggagtaaa acaggtatga cttcatatgt tgataaacaa gaaattacag caagctcaga
     4861 aattgatgat ttgatttttt cgagcgatcc attagtgtgg tcttacgacg agcaggaaaa
     4921 aatcagaaag aaacttgtgc ttgatgcatt tcgtaatcat tataaacatt gtcgagaata
     4981 tcgtcactac tgtcaggcac acaaagtaga tgacaatatt acggaaattg atgacatacc
     5041 tgtattccca acatcggttt ttaagtttac tcgcttatta acttctcagg aaaacgagat
     5101 tgaaagttgg tttaccagta gcggcacgaa tggtttaaaa agtcaggtgg cgcgtgacag
     5161 attaagtatt gagagactct taggctctgt gagttatggc atgaaatatg ttggtagttg
     5221 gtttgatcat caaatagaat tagtcaattt gggaccagat agatttaatg ctcataatat
     5281 ttggtttaaa tatgttatga gtttggtgga attgttatat cctacgacat ttaccgtaac
     5341 agaagaacga atagattttg ttaaaacatt gaatagtctt gaacgaataa aaaatcaagg
     5401 gaaagatctt tgtcttattg gttcgccata ctttatttat ttactctgcc attatatgaa
     5461 agataaaaaa atctcatttt ctggagataa aagcctttat atcataaccg gaggcggctg
     5521 gaaaagttac gaaaaagaat ctctgaaacg tgatgatttc aatcatcttt tatttgatac
     5581 tttcaatctc agtgatatta gtcagatccg agatatattt aatcaagttg aactcaacac
     5641 ttgtttcttt gaggatgaaa tgcagcgtaa acatgttccg ccgtgggtat atgcgcgagc
     5701 gcttgatcct gaaacgttga aacctgtacc tgatggaacg ccggggttga tgagttatat
     5761 ggatgcgtca gcaaccagtt atccagcatt tattgttacc gatgatgtcg ggataattag
     5821 cagagaatat ggtaagtatc ccggcgtgct cgttgaaatt ttacgtcgcg tcaatacgag
     5881 gacgcagaaa gggtgtgctt taagcttaac cgaagcgttt gatagttgag tcgacctaag
     5941 gagaaagaaa tggtgaagat acagcccatc cccacaacta gccagggcag cctttttata
     6001 atgaatagca ccatagagac aatcctgggc catagatcca ttaggaagtt cacatctgaa
     6061 cctattgcta gtgagcagct gcaaacgatt cttcagtctg ggctcgctgc ttcaagctca
     6121 tccatgctgc aggttgtgag tataattcgg gttacagaca cggaaaagag aaaattgctc
     6181 gctcaatatg ccggcaacca gacgtatgtg gaatccgctg ctgagttcct ggtcttttgt
     6241 atagactacc agcgacacgc tactatcaac cccgatgtcc aagctgactt taccgagctg
     6301 accctgattg gtgcagtgga ttccggcata atggcccaga attgcctcct ggcagcagaa
     6361 tcaatgggtc ttggcggagt ctatatcgga ggacttcgga actcagctgc ccaagtggat
     6421 gagttgctcg gactgcccaa gaacacagct atcctcttcg gaatgtgctt ggggcacccc
     6481 gatcagagcc ctgagacaaa gcctagactg cccgctcatg tgatcgtgca cgagaaccaa
     6541 tatcaagctc tgaacattga cgacgtacag gcgtatgaca aaacattgca ggagtattat
     6601 gccagcagaa ccagcaacca gaagcagagt gtctggtccc aggaaactgc aggcaagctg
     6661 gccggagaat ccctcccaca catcctgcca tacctgaact ccaagggcct tgccagaagg
     6721 taagcggccg catcgtgact gactgacgat ctgcctcgcg cgtttcggtg atgacggtga
     6781 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     6841 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     6901 gacccagtca cgtagcgata gcggagtgta taattcttga agacgaaagg gcctcgtgat
     6961 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     7021 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7081 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7141 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7201 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7261 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7321 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7381 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7441 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7501 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7561 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7621 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7681 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7741 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7801 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7861 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7921 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7981 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8041 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8101 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8161 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8221 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8281 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     8341 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8401 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8461 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8521 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8581 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8641 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8701 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8761 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8821 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8881 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8941 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataaa
     9001 ttccgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
     9061 gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc
     9121 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
     9181 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
     9241 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
     9301 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
     9361 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
     9421 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
     9481 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
     9541 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
     9601 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
     9661 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
     9721 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
     9781 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
     9841 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
     9901 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
     9961 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
    10021 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccacgc tagcgcccaa
    10081 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    10141 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtaagttag ctcactcatt
    10201 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    10261 gataacaatt tcacacagga aacagctatg accatgatta cggattcact ggccgtcgtt
    10321 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
    10381 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
    10441 ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
    10501 gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg
    10561 cagatgcacg gttacgatgc gcccatctac accaacgtga cctatcccat tacggtcaat
    10621 ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat
    10681 gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttgg aattagctta
    10741 tcgactgcac ggtgcaccaa tgcttctggc gtcaggcagc catcggaagc tgtggtatgg
    10801 ctgtgcaggt cgtaaatcac tgcataattc gtgtcgctca aggcgcactc ccgttctgga
    10861 taatgttttt tgc
//
