LOCUS       sequence-207672-        3858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HSL relay device: produces 3O-C12-HSL in response to 3O-C6-HSL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Grant PK, Dalchau N, Brown JR, Federici F, Rudge TJ, Yordanov B, 
            Patange O, Phillips A, Haseloff J
  TITLE     Orthogonal intercellular signaling for programmed spatial behavior.
  JOURNAL   Mol Syst Biol. 2016 Jan 25;12(1):849.
  PUBMED    26814193
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2016-01-25"; volume: "12(1)"; pages: "849"
COMMENT     Sequence Label: pLux76-LasI
FEATURES             Location/Qualifiers
     source          1..3858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1412..1603
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1597..1619)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1705..1845
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1760..1779
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1860..1877)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2031..2619)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2111..2130)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2771..3562,3563..3631))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3394..3413
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3632..3736)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3804..3822
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    3837..3858
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 acctgtagga tcgtacaagt ttacgcaaga aaatggtttg ttatagtcga ataaaatact
       61 agagcgcacg cttaaccaag taaaagggtt cgttttttac tagatgattg ttcagattgg
      121 ccgtcgtgag gaatttgata aaaagctgct gggcgagatg cacaaactgc gtgcacaggt
      181 tttcaaggag cgtaaaggtt gggacgttag cgttattgac gaaatggaga ttgatggcta
      241 cgacgctctg tctccgtatt atatgctgat tcaggaggac accccggaag ctcaggtatt
      301 tggttgctgg cgtatcttcg atactacggg tccgtatatg ctgaaaaata ccttcccgga
      361 actgctgcac ggtaaagaag cgccttgtag cccgcacatc tgggaactgt ctcgtttcgc
      421 tatcaactcc ggccagaaag gttccctggg cttttccgat tgtaccctgg aagcaatgcg
      481 tgcgctggcg cgctattcac tgcaaaacga catccagact ctggtgaccg ttactactgt
      541 gggcgttgaa aagatgatga tccgtgcagg cctggacgtt agccgtttcg gtccacacct
      601 gaagatcggt atcgaacgcg cggtggccct gcgtatcgaa ctgaatgcaa aaacccagat
      661 cgcactgtat ggtggtgttc tggtggaaca acgcctggcc gtttcctaat aactcggtac
      721 caaattccag aaaagaggcc tcccgaaagg ggggcctttt ttcgttttgg tccatggaag
      781 ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg
      841 gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc
      901 cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacggg tgcgcatgat
      961 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa
     1021 tgaatcaccg atacacgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg
     1081 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc
     1141 agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg
     1201 aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc
     1261 ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc
     1321 atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat
     1381 gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa
     1441 catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga
     1501 cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg
     1561 cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     1621 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     1681 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     1741 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     1801 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     1861 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1921 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1981 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2041 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2101 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2161 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2221 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2281 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2341 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2401 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2461 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2521 agagttggta gttcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2581 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2641 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2701 tcaaaaagga tcttcaccta gatcctttta aatcaatcta aagtatatat gagtaaactt
     2761 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2821 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2881 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2941 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3001 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3061 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3121 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3181 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3241 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3301 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3361 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3421 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3481 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3541 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3601 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3661 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3721 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     3781 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat
     3841 tcgcggccgc ttctagag
//
