LOCUS       sequence-207673-        3814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HSL relay device: produces 3O-C6-HSL in response to 3O-C12-HSL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Grant PK, Dalchau N, Brown JR, Federici F, Rudge TJ, Yordanov B, 
            Patange O, Phillips A, Haseloff J
  TITLE     Orthogonal intercellular signaling for programmed spatial behavior.
  JOURNAL   Mol Syst Biol. 2016 Jan 25;12(1):849.
  PUBMED    26814193
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2016-01-25"; volume: "12(1)"; pages: "849"
COMMENT     Sequence Label: pLas81-LuxI
FEATURES             Location/Qualifiers
     source          1..3814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1368..1559
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1553..1575)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1661..1801
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1716..1735
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1816..1833)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1987..2575)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2067..2086)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2727..3518,3519..3587))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3350..3369
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3588..3692)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3760..3778
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    3793..3814
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 acctatagga tcgtataggt ttacgcaaga aaatggtttg ttatagtcga ataaaatact
       61 agagtcacac aggaaagtac tagatgacta taatgataaa aaaatcggat tttttggcaa
      121 ttccatcgga ggagtataaa ggtattctaa gtcttcgtta tcaagtgttt aagcaaagac
      181 ttgagtggga cttagttgta gaaaataacc ttgaatcaga tgagtatgat aactcaaatg
      241 cagaatatat ttatgcttgt gatgatactg aaaatgtaag tggatgctgg cgtttattac
      301 ctacaacagg tgattatatg ctgaaaagtg tttttcctga attgcttggt caacagagtg
      361 ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc tgtaggtaaa aatagctcaa
      421 agataaataa ctctgctagt gaaattacaa tgaaactatt tgaagctata tataaacacg
      481 ctgttagtca aggtattaca gaatatgtaa cagtaacatc aacagcaata gagcgatttt
      541 taaagcgtat taaagttcct tgtcatcgta ttggagacaa agaaattcat gtattaggtg
      601 atactaaatc ggttgtattg tctatgccta ttaatgaaca gtttaaaaaa gcagtcttaa
      661 attaataact cggtaccaaa ttccagaaaa gaggcctccc gaaagggggg ccttttttcg
      721 ttttggtcca tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag
      781 ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca
      841 tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc
      901 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
      961 ttactggtta gcagaatgaa tcaccgatac acgagcgaac gtgaagcgac tgctgctgca
     1021 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
     1081 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct
     1141 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag
     1201 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
     1261 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
     1321 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg
     1381 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga
     1441 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg
     1501 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     1561 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     1621 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     1681 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     1741 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     1801 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1861 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1921 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1981 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2041 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2101 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2161 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2221 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2281 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2341 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2401 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     2461 agttaccttc ggaaaaagag ttggtagttc ttgatccggc aaacaaacca ccgctggtag
     2521 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2581 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2641 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatc aatctaaagt
     2701 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2761 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2821 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2881 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2941 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3001 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3061 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3121 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3181 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3241 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3301 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3361 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3421 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3481 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3541 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3601 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3661 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3721 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     3781 tttcgtcttc aagaattcgc ggccgcttct agag
//
