LOCUS       sequence-207687-        9375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian retroviral expression of glutaminase 2 isoform GAB (wild 
            type) with V5 and 6xHis tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Dias MM, Adamoski D, Dos Reis LM, Ascencao CFR, de Oliveira KRS, 
            Mafra ACP, da Silva Bastos AC, Quintero M, de G Cassago C, Ferreira 
            IM, Fidelis CHV, Rocco SA, Bajgelman MC, Stine Z, Berindan-Neagoe I,
            Calin GA, Ambrosio ALB, Dias SMG
  TITLE     GLS2 is protumorigenic in breast cancers.
  JOURNAL   Oncogene. 2020 Jan;39(3):690-702. doi: 10.1038/s41388-019-1007-z. 
            Epub 2019 Sep 20.
  PUBMED    31541193
REFERENCE   2  (bases 1 to 9375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41388-019-1007-z"; journalName: "Oncogene"; date: 
            "2020-01"; volume: "39"; issue: "3"; pages: "690-702"
COMMENT     Sequence Label: pQC hGAB.wt V5-His IRES G418
FEATURES             Location/Qualifiers
     source          1..9375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4167..4208
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4218..4235
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(4284..4857,4858..4860,4861..4869)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4451..4468)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4678..4697
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4870..5664
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4924..4943)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5534..5553
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6081..6506
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6609..6631)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6767..6787)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6772..7101
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6952..7087
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7123..7142
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7223..7240)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7394..7982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7474..7493)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8153..8944,8945..9013))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8776..8795
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9014..9118)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9186..9204
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9304..9375,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca ccggggatcc actagtccag tgtggtggaa
     2281 ttgcccttac catgggccgc tccatgaagg ctctgcagaa ggccctgagc cgggctggca
     2341 gtcactgcgg gcgaggaggc tggggtcacc cgagccggag ccccctcctt ggcgggggcg
     2401 tccggcacca cctcagtgag gccgcggcgc agggcagaga gacgccacac agccaccagc
     2461 cgcagcacca ggatcatgat tcatcagaaa gtggcatgct gtcccgcctg ggtgatttgc
     2521 tcttttacac tattgctgaa ggacaggaac gaatccctat ccacaagttc accactgcac
     2581 taaaggccac tggactgcag acatcagatc ctcggctccg agactgcatg agcgagatgc
     2641 accgcgtggt ccaagagtcc agtagtggtg gcctcttgga ccgagatctc ttccgaaagt
     2701 gtgtgagcag caacattgtg ctcctgaccc aggcattccg aaagaagttt gtcattcctg
     2761 attttgagga gttcacgggc catgtggatc gcatctttga ggatgtcaaa gagctcactg
     2821 gaggcaaagt ggcagcctac atccctcagc tggccaagtc aaacccagac ctgtggggtg
     2881 tctccctgtg cactgtggat ggtcaacggc actctgtggg ccacacaaag atccccttct
     2941 gcctgcagtc ctgtgtgaag cccctcacct atgccatctc cataagcacc ctaggcactg
     3001 actacgtgca caagtttgtg ggcaaagagc caagtggcct gcgctacaac aagctctccc
     3061 tcaatgagga aggaatcccc cataacccca tggtcaatgc tggtgccatt gttgtcagct
     3121 ccctgatcaa gatggactgt aacaaagcag agaagtttga ttttgtgttg cagtatctca
     3181 acaaaatggc tgggaatgaa tacatgggtt tcagcaatgc cacattccag tcagagaagg
     3241 aaacagggga tcggaattat gccatcggct attatctcaa ggaaaagaag tgctttccta
     3301 agggggtgga catgatggct gcccttgatc tctacttcca gctgtgttct gtggaggtca
     3361 cttgtgaatc aggcagtgtc atggcagcca ccctcgccaa cggtgggatc tgccccatca
     3421 caggcgagag tgtgctgagt gctgaagcag tgcgcaacac cctcagcctc atgcattcct
     3481 gcggcatgta tgacttctct ggccagtttg ccttccacgt gggcctgcca gccaagtcag
     3541 ctgtatcagg agccatcctc ctggtggtac ccaatgtcat gggaatgatg tgcctgtcac
     3601 ccccattgga caagctgggg aacagccata gggggaccag cttctgccag aagttggtgt
     3661 ctctcttcaa tttccacaac tatgacaacc tgaggcactg tgctcggaag ttagacccac
     3721 ggcgtgaagg ggcagaaatt cggaacaaga ctgtggtcaa cctgttattt gctgcctata
     3781 gtggcgatgt ctcagctctt cgaaggtttg ccttgtcagc catggatatg gaacagaaag
     3841 actatgactc gcgcacagct ctgcatgttg ctgcagctga aggacacatc gaagttgtta
     3901 aattcctgat cgaggcttgc aaagtgaatc cttttgccaa ggacaggtgg ggcaacattc
     3961 ccctggatga tgctgtgcag ttcaaccatc tggaggtggt caaactgctt caagattacc
     4021 aggactccta cacactctct gaaactcagg ctgaggcagc agctgaggcc ctgtccaaag
     4081 agaacttaga aagcatggta aagggcaatt ctgcagatat ccagcacagt ggcggccgct
     4141 cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg
     4201 attctacgcg taccggtcat catcaccatc accattgagt ttaaactcca aagcatcgat
     4261 ggtagcggcc gcggtgaatt ccgcccctct ccctcccccc cccctaacgt tactggccga
     4321 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     4381 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     4441 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     4501 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     4561 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     4621 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     4681 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     4741 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac
     4801 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt
     4861 gccacaacca tggctgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     4921 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     4981 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     5041 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     5101 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     5161 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     5221 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     5281 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     5341 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     5401 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     5461 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     5521 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     5581 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     5641 cgccttcttg acgagttctt ctgagcggga tcggctagag tcgacccgga cgggacgctc
     5701 ctgcgcctga tacagaacga attgcttgca ggcatctcat gagtgtgtct tcccgttttc
     5761 cgcctgaggt cactgcgtgg atggagcgct ggcgcctgct gcgcgacggc gagctgctca
     5821 ccacccactc gagggctgca gcgctgcaga ggccgagtgc agaactgctc caaagggacc
     5881 tcaaggcttt ccgagggaca ctaggctgac tccatcgagc cagtgtagag ataagcttat
     5941 cgattagtcc aatttgttaa agacaggata tcagtggtcc aggctctagt tttgactcaa
     6001 caatatcacc agctgaagcc tatagagtac gagccataga taaaataaaa gattttattt
     6061 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     6121 gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca
     6181 aggtcaggaa cagatggaac agggtcgacc ctagagaacc atcagatgtt tccagggtgc
     6241 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
     6301 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
     6361 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
     6421 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
     6481 ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc
     6541 agggaccacc gacccaccac cgggaggtaa gctggctgcc tcgcgcgttt cggtgatgac
     6601 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     6661 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     6721 gccatgaccc agtcacgtag cgatagcgga gtgtagatcc ggctgtggaa tgtgtgtcag
     6781 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6841 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6901 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6961 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7021 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7081 ggaggcctag gcttttgcaa aaagcttact ggcttaacta tgcggcatca gagcagattg
     7141 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     7201 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7261 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7321 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7381 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7441 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7501 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7561 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7621 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7681 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7741 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7801 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     7861 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7921 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7981 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8041 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8101 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8161 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8221 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8281 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8341 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8401 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8461 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8521 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8581 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8641 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8701 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8761 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8821 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8881 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8941 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9001 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9061 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9121 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     9181 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attagcttgg ccattgcata
     9241 cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca ttaccgccat
     9301 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     9361 gcccatatat ggagt
//
