LOCUS       sequence-207689-       10194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in
            nematode. GFP is optimized by removing all piRNA targeting sites to 
            allow germline expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Zhang D, Tu S, Stubna M, Wu WS, Huang WC, Weng Z, Lee HC
  TITLE     The piRNA targeting rules and the resistance to piRNA silencing in 
            endogenous genes.
  JOURNAL   Science. 2018 Feb 2;359(6375):587-592. doi: 10.1126/science.aao2840.
            Epub 2018 Feb 1.
  PUBMED    29420292
REFERENCE   2  (bases 1 to 10194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aao2840"; journalName: "Science"; date: "2018-02-2-
            2"; volume: "359"; issue: "6375"; pages: "587-592"
COMMENT     Sequence Label: pCFJ150-GFP(dpiRNA)::CDK-1
FEATURES             Location/Qualifiers
     source          1..10194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(146..734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(226..245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(905..1696,1697..1765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1528..1547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1766..1870)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1938..1956
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1994..2016)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             3832..3843
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             8693..8704
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tgtgaaattg ttatccgctg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
       61 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag accaggaacc
      121 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      781 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
      961 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1021 accccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1081 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1141 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1201 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1261 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1321 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1381 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1441 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1501 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1561 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1621 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1681 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1741 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1801 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1861 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     1921 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     1981 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     2041 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2101 gctggcttaa ctatgcggca tcagagcaga ttgcactgag agtgcaccat atgcggtgtg
     2161 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     2221 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2281 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc ccagtcacga
     2341 cgtcacagca cgtgtgtttt tgaacacggc gatatgtatc tgtagatcat ccccaatttt
     2401 caacaatgaa tctaaatact gaactttttg tgtaatcttt agtgcagaaa aatgaccaaa
     2461 atcagcgatt ttccgattat acccaaaata tctcagctca atgagcacct ataaaaatgt
     2521 aaaaataaag aaagtagtcg aaccatattt tgtaaaggct aaaaaactga ataaattttt
     2581 atccatccag cgcggcgtta caatccgtgg cccatgtcac gcacggcttt caaagaaaaa
     2641 aaaacagtct gtttgcgcga catcgatctg gcaaaaatcc taaaaacgac tatgcgtggc
     2701 tcactttata ttaatatgaa aaatacaaac ttggtggtgc aggtgctgca gaggccatcg
     2761 atgaagaagt ggaggccttg ttgtcgatct acaaaattgt atggttgttt gagattttcc
     2821 ttgaaacgct ctcaatcttg gatatgatat aaattctgga taagatatga tggatatgat
     2881 ctatgtttga tagagtgcat ttgaaaggat aacggccctg cagacatttt tgaaaaaata
     2941 acaaacattt aattttctca ctcgtttagg ctattcccct atttttgata ttccttcgca
     3001 catatgaaaa ctactttttt tcgaaactgt taactccaga atttataaat ctatagccct
     3061 tacttgatta tttattatca tggttactca cctcatgtct ctttctcttt tcaagaagtc
     3121 ttcgttcata tctgttgtct ctttcctcct tttgaatctc tgctttgctc ttcgccattg
     3181 tttcctgaaa ataatgtaac ttgaattgtg taatatactt ttttaatttg aatttggctt
     3241 gtaacgcgga atcactacgt gcgggatcat ttcttactag aaaacccaga aaatgccata
     3301 tttcacttat ctcggggtca tttctaatta gaaaagctac aaaaaccatt tctaattacg
     3361 gtgactttag aactggtcga aatagctaat tagaaatggg tttttgctaa ttagaggtga
     3421 ctctatgtta tctgagtaag cctcgtttgt atgtaaactc acagtactaa taaatgcaag
     3481 acacccgggt ttgtctagat atgaaataat tgaaatatca attctgacag acaataatgg
     3541 taatcttgat aaggagttcc acgcccagga gaacacgtta gttttcttgt ttttgattgc
     3601 gtgcgttatt ttggagaaaa actcgatttt ttacaaaata attttttgaa aggaacactg
     3661 ttcaataagt tttgtctttt ttctcagttg tgatacggtt ttttattctt ttttgtagtt
     3721 atacagaaga ccgttacgaa acagactgat atcgaaacaa acactgatct tacttgcact
     3781 tataatacga ctcactagtg ggcagatctg ggtaccggtg gtggctccgg tattgaggga
     3841 cgcgggtcca tggatatcgg ggatccgaat tctgtacagg ccttggcgcg cctgcaggcg
     3901 agctccgtcg acaagcttgc ggccgcggaa gcctcataaa gtagaacaaa tcaagtttat
     3961 gattattttg agtagttatt tttatatttt ccggcctgta tttattctct tttctgcaca
     4021 gttttgcgtt aaattttatc atatgaatgc aatgtttcac aacaatcctt ctcagcgcgt
     4081 actctccttt tcggaataaa ttgtcatcaa aggtgcatgc cttttcacta agcaatggtc
     4141 tcgcaccgac tgtcccccct tttcggcggc agtggaaatg attcaaaatt acacgcttac
     4201 gccttttcta tcgttgtatt acaattgttc tgacaaaatt catttccaat tttagttatg
     4261 agtaaaggag aagaactttt cactggagtt gtcccaatcc ttgttgaatt agatggtgat
     4321 gttaatgggc acaaattttc cgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     4381 cttaccctta aatttatctg cactactgga aaactacctg ttccatgggt aagtttaaac
     4441 atatatatac taactaaccc tgattattta aattttcagc caacacttgt cactactctc
     4501 acttatggtg ttcaatgctt ctcgagatac ccagatcata tgaaacagca tgacttcttc
     4561 aagagtgcca tgcccgaagg ttatgtacag gaaagaacaa tatttttcaa agacgacggg
     4621 aactacaaga cacgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt
     4681 tcaggtgctg aagtcaagtt tgaaggagac acccttgtca atagaatcga gttaaaaggt
     4741 attgatttta aagaagacgg caacattctt ggccacaaat tggaatacaa ctataactca
     4801 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttgt aagtttaaac
     4861 atgattttac taactaacta atctgattta aattttcaga acttcaaaat tagacacaac
     4921 attgaagatg gaagcgtaca actagcagac cattatcaac aaaatactcc aataggagat
     4981 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttctaaagac
     5041 cccaacgaaa agagagacca catggtcctt cttgagttcg taacagctgc tgggattaca
     5101 catggcatgg atgaactata caaagcatct gatcctattc gcgaaggaga agtggcccac
     5161 gagggagatt cggtttacac actcaacgat ttcacgaagc tcgaaaaaat cggcgaagga
     5221 acatacggag tcgtctacaa aggcaaaaac agaagaacca atgccatggt tgctatgaag
     5281 aaaatcagat tggaaagtga agatgaggta tttgaatttt gtagcagttt cgttttttca
     5341 aacaataatt cttatagggt gttccatcaa cggctgtgcg agagatcagc ttgctcaaag
     5401 agctccagca tccgaatgtt gttggattgg aagcggtcat tatgcaggag aaccggcttt
     5461 tcctgatctt cgaattcctg tctttcgact tgaaacgata catggatcag ttgggaaaag
     5521 atgaatacct tccgctcgaa actcttaaaa gttacacatt ccaagtgagg aaatatttaa
     5581 aatcgatgtc tttacaagaa taattgttcc agattcttca agctatgtgt ttttgtcatc
     5641 agcgtcgtgt tattcatcgc gatcttaagc cacagaactt gctggtcgac aacaatggag
     5701 caatcaagct tgccgatttc ggacttgcca gagctattgg tatcccgatt cgcgtttaca
     5761 cgcatgaagt gagtttgcat tcattctgta caccaataca atgattgtcc ggttttcagg
     5821 ttgtcactct ctggtataga gctccagaaa ttctgatggg tgctcagaga tactcgatgg
     5881 gcgtagacat gtggtcgatt ggatgcattt ttgctgaaat ggccacgaag aagccactgt
     5941 ttcaaggaga ttcagaaatt gatgagctgt tccgcatctt cagagttctc ggcacaccga
     6001 ctgagttgga atggaatggt gttgaatcac ttccggacta caaggcgacg ttcccaaaat
     6061 ggcgtgagaa tttcctccgt gacaagttct acgacaagaa gactggaaag caccttttgg
     6121 acgatactgc attctctctt cttgaagtaa gatttctaaa atagtacttt aacaagtaaa
     6181 ataatatttt cagggtttgc tgatctacga tccatcactt cgtctcaacg cgaaaaaagc
     6241 tctcgttcat ccttattttg acaacatgga tacgtcaaag ctgccagctg gaaattaccg
     6301 aggagaactc gaactctttt gatgtaattc attcatcatc atttttatta tttattctcc
     6361 cgtgctttaa ccacctatcg tcttccgtgt attgtttaac tatcaccatt tcaacttttt
     6421 acctgtacga tgattatttg gcccttcgat attcatgagt aatctctttt ttcacaccgc
     6481 gataaataaa ttcgctctac tttcatgctc ttttttattc aagcactttg ttcaacgtgt
     6541 aaatgagaat agggaattaa gaattcatta ttattatttt tccattctga agaatgatcg
     6601 ctgcagtgat tccaatattc gtaatttctc tgtctaacac tcgatggggg gcccggtacc
     6661 aacctaggtg agctctggta ccctctagtc aaggccttaa gtgagtcgta ttacgtagcc
     6721 cgggcctagt tctagacatt ctctaatgaa aaaatctttc agttgaaatt gaaaatgagt
     6781 taaagttgga gtttttattg aaaacagatt tccgtgtgat tagtgttttt agcgagtgtg
     6841 acaggacagc gaaaaaatat agaaacaagg ggggaactga aaagcttagg aatgcattga
     6901 acatgagaag gggaagggga aggaacaaac tagacaggaa ttattggaat ttaatcacat
     6961 ttggagtttt ttttctattc gacagaataa ttatccagaa catttttgta ttaaatattt
     7021 atgcatcata tgagtagtcg gctttgttgt gcatgacgag tttgttatcg acgaaataga
     7081 agctgtcaga acgagtctcg tttggattgt tgatcatgtc gtccactgaa aaagagatta
     7141 gtcttttgaa ttgtactttt tagaataatg actcactgag ttgttgagag agttgaggga
     7201 actcatagat atgttcacag ttgtttcgtg aattcggaat acagaatccg aattcaaagt
     7261 caaaacactt cagaaggcga tctttgaaga agtgacgttc gatcattcgg aaatgatgga
     7321 ttgggatgtc tcctactttg aattccacag ttgctccgac cgttttgagt ttcaaaaagt
     7381 ttggagcaaa tctataacgc acgtatcttg ccgactcctg tggcgactca tcattctcct
     7441 gatcgttctc cggtttggcg atctcgaaaa gcacttgctc agtgtccaga tcacggattt
     7501 ggaacttggt gaactcgatg ttatagatgt tcgcagatgg ggagcataag aatcctaaat
     7561 ttatgtttta aactgaaatc caaagggagc aagatacctt gagtgattcc cggaagtgct
     7621 aaaacgtcgt tcggagtgat ttgagctttc ttcgcaagct ccgattccgt tgtgattcct
     7681 tgttcggtgc ttggtggtgg ccgtggcatc tggaaatatg gaaaagttca acaaaaagaa
     7741 aagagaaaag aatgaaatcg gatatcaaga gttagttgag cggtttctct agttttctga
     7801 gtctcacctg cgacgggaag gtcgccgagc cgggtggaat cgatcgttgt tgctcggctt
     7861 tcatatcggt ttggttggaa gcggctgaaa acggaaagaa gtggaagaag gaaaagagtg
     7921 tggtgtgaca ggaaaatggt aattagaggg tgccaaataa ccagctatat tttgtttttt
     7981 tttgaaaaca tttttaaaaa gaaaaatacg ataatgatat cagatggatt tccggaaaac
     8041 tggtatgaaa aatttcaacc tttttgagta catgtaatca aaatacactt tgtaaattat
     8101 catttttatt gaaactccac catttttcta tttataacgc taataatttg aaaaagaaac
     8161 ctattgcgaa ccgcggggtg aatcccaaaa acgaatgcgt tttggtggag tgattgattc
     8221 gaatcgaaga agaaaaagaa gaagacgtgg aatagagagc tcactcttaa ccgagcagca
     8281 cacaccgaca gaaaaaaaaa tgaaatgaat gagggtcttc ttcttcttct tcttcgaatg
     8341 attgacagaa atgggaaaaa gaggaagatt gagaagggaa aaaggaagga gaaaagaagc
     8401 agaagaagac gtcagagagg agaggaacga gcggaaaagc agcgggcgca agtcatagaa
     8461 gtagcagagc tggggagaag aagacactat ccaagaaagg aatgacgaga gagtatgcaa
     8521 aggggtatag ggtgcagaca gaataggaac agaataacag atgatgagcc aagaagagtt
     8581 gaaaagggcg atgaatttgt catgtaactt aatttgggtc aatttgagca tgatgaattg
     8641 aaatcatccc ttgttgggag ttaataaccg gtttgttatc agaaaccctg taatagaagg
     8701 gcgccctaac tttgagccaa ttcatcccgg tttctgtcaa atatatcaaa aagtggtcaa
     8761 ctgacaaatt gtttttgata ttataataaa cattttatcc gttaacaatt ttcgaatact
     8821 ttttacaagg acttggataa attggctcaa agacgaagtg agtttgctac catcatggaa
     8881 tcaggaggac atttcgacaa tgtcatataa tgtttgaaaa atgattttaa caagggtctc
     8941 attcgagata agcaatgctc tgagcaagtg gctcgtcgtc ttcaagaatc tcttgttgat
     9001 attcatttgg atcctgtttt gcacgaagca tgtgccatgg atattcagag tttttgcaaa
     9061 aaagttccgc caggtcaaac aatcttttta atttttatat gatacttcgg gtgtattttt
     9121 cttctttttc ctgttcattt tcgctttttt ctcacttttt tgtaacaaaa tctctcactt
     9181 ttctgtaagt cttaacaaaa gttcaattgg ctcgatcaag taaaatttcg gccgtatgaa
     9241 cgttttcaaa ggttttcatt tttttttaag ttcattcaca atatcatgaa tctctcgtcc
     9301 tttggtttcc ggtaagtagc gaaataatat aagcatactt ataaatgaag gaattgaata
     9361 tagaataagg aaagcataac taccaataac ggaatataga ggtaatactg taaatgtact
     9421 gataaccacc attacagtat tcattgaata tgccacagat tgagcaatac tacgatgttt
     9481 ttgaggtacc agttcagagg atataaacca cgaaattgga ccaactccag ttctgaaatt
     9541 tcattattcg attaaatcaa aatatatttg aaaaacctca cccataagta taaccgtaga
     9601 tgatcaatgc aactaggcat ccgtatttca tcgggtcaat cagaatacgt atttttgcga
     9661 agaatacgaa tgcaattaga gctaaattat tgagaaatgt gaagagaagg agaagcttgc
     9721 gtcgaccaac tctgtaaact tattcataca attttgtctt caaattttga acgtttaaaa
     9781 ctatcttatt caatgagtta gcgggttttt atagtgagtt agttcacgat tatctaataa
     9841 aactatgtcc aaacatccta actgcgaccg tattctcact aactcgtctt aaaaacgttg
     9901 agattatttt aaacccttct tcagtacaac tacagtactg atgcacaaat acaataataa
     9961 taatacattc agacatactc tgaacaatgt aacttgtttt tgttatactg tggtaacatt
    10021 tattgaaatg aaatataacc ttacttttca atcacaattc caccagtaat tgttccagaa
    10081 acataagcta aacccattgc agttgtactc cattcagcaa tttctgactc aacatttgca
    10141 ttttctagaa agaaagtaga ggataaaaga agagcccata atccgacagg gccc
//
