LOCUS       sequence-20773-e         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG424GPD-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(127..251)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             310..387
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             415..435
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctcgttatcg gggaaaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
       61 ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag
      121 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
      181 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg
      241 tacaaagtgg tgatgggctg caggaattcg atatcaagct tatcgatagg tggaccaggt
      301 ggtggaatga agcgacgatg gaaaaagaat ttcatagccg tctcagcagc caaccgcttt
      361 aagaaaatct catcctccgg ggcacttgat tatggtaccc caactactgc ttctgaaaat
      421 ctatattttc aaggtgaact aaaaactgct gctttggctc aacatgcgat taagggtgaa
      481 gctcaaaaac ttaatgactc tcaagctcca aaagctgatg cgcaacaaaa taacttcaac
      541 aaagatcaac aaagcgcctt ctatgaaatc ttgaacatgc ctaacttaaa cgaagcgcaa
      601 cgtaacggct tcattcaaag tcttaaagac gacccaagcc aaagcactaa cgttttaggt
      661 gaagctaaa
//
