LOCUS       sequence-207768-        6857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for mCherry fusion to human Hsp90 beta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Picard D, Suslova E, Briand PA
  TITLE     2-color photobleaching experiments reveal distinct intracellular 
            dynamics of two components of the Hsp90 complex.
  JOURNAL   Exp Cell Res. 2006 Nov 15. 312(19):3949-58.
  PUBMED    17010336
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp Cell 
            Res. 2006 Nov 15. 312(19):3949-58."
COMMENT     Sequence Label: pCherry.90 beta
FEATURES             Location/Qualifiers
     source          1..6857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        88..391
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        392..595
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     546..566
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      622..631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             628..1335
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(773..791)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1041..1060
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3672..3793
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3709..3728)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3763..3782
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3800..4255)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3937..3958)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4149..4168
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4282..4386
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4388..4745
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4388..4579
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4411..4431)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4596..4731
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4780..5574
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4780..5574
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4834..4853)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5444..5463
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5762..5781)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5806..5853
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6182..6770
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6671..6690
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt
       61 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      121 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      181 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      241 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      301 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      361 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      421 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      481 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      541 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
      601 agtgaaccgt cagatccgct agccaccatg gtgagcaagg gcgaggagga taacatggcc
      661 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
      721 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
      781 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
      841 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
      901 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
      961 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     1021 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     1081 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     1141 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     1201 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     1261 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     1321 gacgagctgt acaagagatc tggaggtatg cctgaggaag tgcaccatgg agaggaggag
     1381 gtggagactt ttgcctttca ggcagaaatt gcccaactca tgtccctcat catcaatacc
     1441 ttctattcca acaaggagat tttccttcgg gagttgatct ctaatgcttc tgatgccttg
     1501 gacaagattc gctatgagag cctgacagac ccttcgaagt tggacagtgg taaagagctg
     1561 aaaattgaca tcatccccaa ccctcaggaa cgtaccctga ctttggtaga cacaggcatt
     1621 ggcatgacca aagctgatct cataaataat ttgggaacca ttgccaagtc tggtactaaa
     1681 gcattcatgg aggctcttca ggctggtgca gacatctcca tgattgggca gtttggtgtt
     1741 ggcttttatt ctgcctactt ggtggcagag aaagtggttg tgatcacaaa gcacaacgat
     1801 gatgaacagt atgcttggga gtcttctgct ggaggttcct tcactgtgcg tgctgaccat
     1861 ggtgagccca ttggcagggg taccaaagtg atcctccatc ttaaagaaga tcagacagag
     1921 tacctagaag agaggcgggt caaagaagta gtgaagaagc attctcagtt cataggctat
     1981 cccatcaccc tttatttgga gaaggaacga gagaaggaaa ttagtgatga tgaggcagag
     2041 gaagagaaag gtgagaaaga agaggaagat aaagatgatg aagaaaagcc caagatcgaa
     2101 gatgtgggtt cagatgagga ggatgacagc ggtaaggata agaagaagaa aactaagaag
     2161 atcaaagaga aatacattga tcaggaagaa ctaaacaaga ccaagcctat ttggaccaga
     2221 aaccctgatg acatcaccca agaggagtat ggagaattct acaagagcct cactaatgac
     2281 tgggaagacc acttggcagt caagcacttt tctgtagaag gtcagttgga attcagggca
     2341 ttgctattta ttcctcgtcg ggctcccttt gacctttttg agaacaagaa gaaaaagaac
     2401 aacatcaaac tctatgtccg ccgtgtgttc atcatggaca gctgtgatga gttgatacca
     2461 gagtatctca attttatccg tggtgtggtt gactctgagg atctgcccct gaacatctcc
     2521 cgagaaatgc tccagcagag caaaatcttg aaagtcattc gcaaaaacat tgttaagaag
     2581 tgccttgagc tcttctctga gctggcagaa gacaaggaga attacaagaa attctatgag
     2641 gcattctcta aaaatctcaa gcttggaatc cacgaagact ccactaaccg ccgccgcctg
     2701 tctgagctgc tgcgctatca tacctcccag tctggagatg agatgacatc tctgtcagag
     2761 tatgtttctc gcatgaagga gacacagaag tccatctatt acatcactgg tgagagcaaa
     2821 gagcaggtgg ccaactcagc ttttgtggag cgagtgcgga aacggggctt cgaggtggta
     2881 tatatgaccg agcccattga cgagtactgt gtgcagcagc tcaaggaatt tgatgggaag
     2941 agcctggtct cagttaccaa ggagggtctg gagctgcctg aggatgagga ggagaagaag
     3001 aagatggaag agagcaaggc aaagtttgag aacctctgca agctcatgaa agaaatctta
     3061 gataagaagg ttgagaaggt gacaatctcc aatagacttg tgtcttcacc ttgctgcatt
     3121 gtgaccagca cctacggctg gacagccaat atggagcgga tcatgaaagc ccaggcactt
     3181 cgggacaact ccaccatggg ctatatgatg gccaaaaagc acctggagat caaccctgac
     3241 caccccattg tggagacgct gcggcagaag gctgaggccg acaagaatga taaggcagtt
     3301 aaggacctgg tggtgctgct gtttgaaacc gccctgctat cttctggctt ttcccttgag
     3361 gatccccaga cccactccaa ccgcatctat cgcatgatca agctaggtct aggtattgat
     3421 gaagatgaag tggcagcaga ggaacccaat gctgcagttc ctgatgagat cccccctctc
     3481 gagggcgatg aggatgcgtc tcgcatggaa gaagtcgatt agtcgacggt accgcgggcc
     3541 cgggatccac cggatctaga taactgatca taatcagcca taccacattt gtagaggttt
     3601 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     3661 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3721 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3781 tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     3841 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     3901 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     3961 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     4021 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     4081 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     4141 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     4201 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt
     4261 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4321 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4381 aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     4441 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4501 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4561 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4621 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4681 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     4741 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     4801 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     4861 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     4921 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
     4981 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     5041 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     5101 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     5161 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     5221 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     5281 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
     5341 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     5401 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     5461 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     5521 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     5581 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     5641 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     5701 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa
     5761 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     5821 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     5881 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     5941 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     6001 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc
     6061 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6121 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6181 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6241 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6301 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6361 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6421 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6481 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6541 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6601 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6661 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6721 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6781 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6841 cgttatcccc tgattct
//
