LOCUS       sequence-207782-        9561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gamma-Retroviral transfer vector for expressing glutminase isoform 
            KGA (S286A), C-terminal V5-6xHis tags, IRES-driven Geneticin 
            selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Sandra Martha Gomes Dias
  TITLE     HuR (ELAVL1) silencing sensitizes triple-negative breast cancer to 
            glutaminase-targeted therapy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pQC hKGA.S286A V5-His IRES G418
FEATURES             Location/Qualifiers
     source          1..9561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4353..4394
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4404..4421
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(4470..5043,5044..5046,5047..5055)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4637..4654)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4864..4883
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5056..5850
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5110..5129)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5720..5739
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6267..6692
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6795..6817)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6953..6973)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6958..7287
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7138..7273
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7309..7328
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7409..7426)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7580..8168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7660..7679)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8339..9130,9131..9199))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8962..8981
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9200..9304)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9372..9390
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9490..9561,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca ccggggatcc agtacccttc accatgatgc
     2281 ggctgcgagg ctcggggatg ctgcgggacc tgctcctgcg gtcgcccgcc ggcgtgagcg
     2341 cgactctgcg gcgggcacag cccttggtca ccctgtgccg gcgtccccga ggcgggggac
     2401 ggccggccgc gggcccggct gccgccgcgc gactccaccc gtggtggggc gggggcggct
     2461 ggccggcgga gcccctcgcg cggggcctgt ccagctctcc ttcggagatc ttgcaggagc
     2521 tgggcaaggg gagcacgcat ccgcagcccg gggtgtcgcc acccgctgcc ccggcggcgc
     2581 ccggccccaa ggacggcccc ggggagacgg acgcgtttgg caacagcgag ggcaaagagc
     2641 tggtggcctc aggtgaaaat aaaataaaac agggtctgtt acctagcttg gaagatttgc
     2701 tgttctatac aattgctgaa ggacaagaga aaatacctgt tcataaattt attacagcac
     2761 tcaaatctac aggattgcga acgtctgatc ccaggttgaa agagtgtatg gatatgttaa
     2821 gattaactct tcaaacaaca tcagatggtg tcatgctaga caaagatctt tttaaaaaat
     2881 gtgttcagag caacattgtt ttgttgacac aagcatttag aagaaagttt gtgattcctg
     2941 actttatgtc ttttacctca cacattgatg agttatatga aagtgctaaa aagcagtctg
     3001 gaggaaaggt tgcagattat attcctcaac tggccaaatt cagtcccgat ttgtggggtg
     3061 tgtctgtttg tacagtagat ggacagaggc attctactgg agataccaaa gttcccttct
     3121 gtcttcaggc ctgtgtaaaa cctttgaaat atgccattgc tgttaatgat cttggaactg
     3181 aatatgtgca tcgatatgtt ggaaaagagc cgagtggact aagattcaac aaactatttt
     3241 tgaatgaaga tgataaacca cataatccta tggtaaatgc tggagcaatt gttgtgactt
     3301 cactaataaa gcaaggagta aataatgctg aaaaatttga ctatgtcatg cagtttttga
     3361 ataagatggc tggtaatgaa tatgttggat tcagtaatgc aacgtttcag tctgaaagag
     3421 aaagtggaga tcgaaatttt gcaataggat attacttaaa agaaaagaag tgttttccag
     3481 aaggcacaga catggttggt atattagact tctacttcca gctgtgctcc attgaagtga
     3541 cttgtgaatc agccagtgtg atggctgcga cactggctaa tggtggtttc tgcccaatta
     3601 ctggtgaaag agtactgagc cctgaagcag ttcgaaatac attgagtttg atgcattcct
     3661 gtggcatgta tgacttctca gggcagtttg ctttccatgt tggtcttcct gcaaaatctg
     3721 gagttgctgg gggcattctt ttagttgtcc ccaatgttat gggtatgatg tgctggtctc
     3781 ctcctctgga taagatgggc aacagtgtta agggaattca cttttgtcac gatcttgttt
     3841 ctctgtgtaa tttccataac tatgataatt tgagacactt tgcaaaaaaa cttgatcctc
     3901 gaagagaagg tggtgatcaa agggtaaagt cagtgataaa tcttttgttt gctgcatata
     3961 ctggagatgt gtctgcactt cgaagatttg ctttgtcagc tatggacatg gaacagcggg
     4021 actatgattc tagaacagca ctccatgtag ctgctgcaga gggtcatgtt gaagttgtta
     4081 aatttttgct ggaagcctgc aaagtaaacc ctttccccaa ggacaggtgg aataacactc
     4141 ccatggatga agcactgcac tttggacacc atgatgtatt taaaattctc caagaatacc
     4201 aagtccagta cacacctcaa ggagattctg acaacgggaa ggaaaatcaa accgtccata
     4261 agaatcttga tggattgttg aagggtcaag acaattctgc agatatccag cacagtggcg
     4321 gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg
     4381 gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa actccaaagc
     4441 atcgatggta gcggccgcgg tgaattccgc ccctctccct cccccccccc taacgttact
     4501 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     4561 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     4621 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     4681 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     4741 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     4801 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     4861 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     4921 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     4981 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     5041 aagcttgcca caaccatggc tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     5101 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     5161 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     5221 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     5281 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     5341 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     5401 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     5461 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     5521 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     5581 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     5641 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     5701 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     5761 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     5821 ttctatcgcc ttcttgacga gttcttctga gcgggatcgg ctagagtcga cccggacggg
     5881 acgctcctgc gcctgataca gaacgaattg cttgcaggca tctcatgagt gtgtcttccc
     5941 gttttccgcc tgaggtcact gcgtggatgg agcgctggcg cctgctgcgc gacggcgagc
     6001 tgctcaccac ccactcgagg gctgcagcgc tgcagaggcc gagtgcagaa ctgctccaaa
     6061 gggacctcaa ggctttccga gggacactag gctgactcca tcgagccagt gtagagataa
     6121 gcttatcgat tagtccaatt tgttaaagac aggatatcag tggtccaggc tctagttttg
     6181 actcaacaat atcaccagct gaagcctata gagtacgagc catagataaa ataaaagatt
     6241 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
     6301 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc
     6361 agatcaaggt caggaacaga tggaacaggg tcgaccctag agaaccatca gatgtttcca
     6421 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
     6481 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
     6541 actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac
     6601 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
     6661 gattgactac ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc
     6721 ctgcccaggg accaccgacc caccaccggg aggtaagctg gctgcctcgc gcgtttcggt
     6781 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     6841 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     6901 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt agatccggct gtggaatgtg
     6961 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7021 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     7081 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     7141 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7201 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7261 ttttttggag gcctaggctt ttgcaaaaag cttactggct taactatgcg gcatcagagc
     7321 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     7381 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7441 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7501 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7561 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7621 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7681 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7741 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7801 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7861 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7921 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7981 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     8041 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8101 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8161 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8221 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8281 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8341 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8401 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8461 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8521 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8581 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8641 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8701 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8761 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8821 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8881 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8941 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9001 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9061 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9121 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9181 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9241 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9301 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     9361 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaatta gcttggccat
     9421 tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac
     9481 cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     9541 ttcatagccc atatatggag t
//
