LOCUS       sequence-20779-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GPD-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(126..250)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             309..386
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             414..434
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
       61 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
      121 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
      181 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
      241 acaaagtggt gatgggctgc aggaattcga tatcaagctt atcgataggt ggaccaggtg
      301 gtggaatgaa gcgacgatgg aaaaagaatt tcatagccgt ctcagcagcc aaccgcttta
      361 agaaaatctc atcctccggg gcacttgatt atggtacccc aactactgct tctgaaaatc
      421 tatattttca aggtgaacta aaaactgctg ctttggctca acatgcgatt aagggtgaag
      481 ctcaaaaact taatgactct caagctccaa aagctgatgc gcaacaaaat aacttcaaca
      541 aagatcaaca aagcgccttc tatgaaatct tgaacatgcc taacttaaac gaagcgcaac
      601 gtaacggctt cattcaaagt cttaaagacg acccaagcca aagcactaac gttttaggtg
      661 aagctaaaaa attaaacgaa tctcaagcac cgaaagctga taacaatttc aacaaagaac
      721 aacaaaatgc tttctatgaa atcttgaata tgc
//
