LOCUS       sequence-20782-e         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG426GAL-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(121..245)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             304..381
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             409..429
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atcggggaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
       61 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
      121 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
      181 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
      241 gtggtgatgg gctgcaggaa ttcgatatca agcttatcga taggtggacc aggtggtgga
      301 atgaagcgac gatggaaaaa gaatttcata gccgtctcag cagccaaccg ctttaagaaa
      361 atctcatcct ccggggcact tgattatggt accccaacta ctgcttctga aaatctatat
      421 tttcaaggtg aactaaaaac tgctgctttg gctcaacatg cgattaaggg tgaagctcaa
      481 aaacttaatg actctcaagc tccaaaagct gatgcgcaac aaaataactt caacaaagat
      541 caacaaagcg ccttctatga aatcttgaac atgcctaact taaacgaagc gcaacgtaac
      601 ggcttcattc aaagtcttaa agacgaccca agccaaagca ctaacgtttt aggtgaagct
      661 aaaaaattaa acgaatctca agcaccgaaa gctgataaca atttcaacaa agaacaacaa
      721 aatgctttct atgaaatctt ga
//
