LOCUS       sequence-207840-       10989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant 
            silent mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Tarumoto Y, Lu B, Somerville TDD, Huang YH, Milazzo JP, Wu XS, 
            Klingbeil O, El Demerdash O, Shi J, Vakoc CR
  TITLE     LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in 
            Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011.
  PUBMED    29526696
REFERENCE   2  (bases 1 to 10989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011."
COMMENT     Sequence Label: LentiV_Neo_SIK3_CR_K95M
FEATURES             Location/Qualifiers
     source          1..10989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        33..259
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             260..440
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    487..612
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1105..1338
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1523..1567
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1751..1868
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1937..2148
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5989..6045
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6064..6858
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6118..6137)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6728..6747
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6874..7462
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6927..6947)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7345..7356)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7534..7767
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7845..7966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7882..7901)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7936..7955
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8006..8141
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8161..8180)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8162..8180)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8190..8207)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8190..8206)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8199..8221)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8348..8803
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8435..8454)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8645..8666
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8829..8933
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8934..9002,9003..9794)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9152..9171)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9965..10553
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10454..10473
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10707..10724
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10841..10862
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10877..10882,10883..10900,10901..10907)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10915..10931
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10920..10942
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10939..10955
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10939..10955
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     join(10974..10989,1..5)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        join(10976..10989,1..5)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
       61 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
      121 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
      181 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
      241 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
      301 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
      361 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
      421 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
      481 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
      541 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
      601 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
      661 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
      721 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
      781 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
      841 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
      901 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
      961 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     1021 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     1081 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     1141 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     1201 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     1261 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     1321 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     1381 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     1441 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     1501 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     1561 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     1621 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     1681 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     1741 cagaccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     1801 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     1861 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgagtggc
     1921 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     1981 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     2041 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     2101 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt gtcgtgacgc
     2161 gggatccgcc acccggaggt ttcactgcac aacaagatgg cggcggcggc ggcgagcgga
     2221 gctggcgggg ctgccggggc cgggactggg ggagccgggc ccgcgggccg cctgctgcct
     2281 ccgcccgcgc cggggtcccc agccgccccc gctgccgtgt cccctgcggc cggccagccg
     2341 cgtcccccag ccccggcctc ccgcggaccc atgcccgccc gtatcggcta ctacgagatc
     2401 gaccgcacca tcggcaaggg caacttcgcg gtggtcaagc gggccacgca cctcgtcacc
     2461 aaggccaagg ttgctatcat gatcatagat aagacccagc tggatgaaga aaacttgaag
     2521 aagattttcc gggaagttca aattatgaag atgctttgcc acccccatat catcaggctc
     2581 taccaggtta tggagacaga acggatgatt tatctggtga cagaatatgc tagtggaggg
     2641 gaaatatttg accacctggt ggcccatggt agaatggcag aaaaggaggc acgtcgcaag
     2701 ttcaaacaga tcgtcacagc tgtctatttt tgtcactgtc ggaacattgt tcatcgtgat
     2761 ttaaaagctg aaaatttact tctggatgcc aatctgaata tcaaaatagc agattttggt
     2821 ttcagtaacc tcttcactcc tgggcagctg ctgaagacct ggtgtggcag ccctccctat
     2881 gctgcacctg aactctttga aggaaaagaa tatgatgggc ccaaagtgga catctggagc
     2941 cttggagttg tcctctacgt gcttgtgtgc ggtgccctgc catttgatgg aagcacactg
     3001 cagaatctgc gggcccgcgt gctgagtgga aagttccgca tcccattttt tatgtccaca
     3061 gaatgtgagc atttgatccg ccatatgttg gtgttagatc ccaataagcg cctctccatg
     3121 gagcagatct gcaagcacaa gtggatgaag ctaggggacg ccgatcccaa ctttgacagg
     3181 ttaatagctg aatgccaaca actaaaggaa gaaagacagg tggaccccct gaatgaggat
     3241 gtcctcttgg ccatggagga catgggactg gacaaagaac agacactgca ggcggagcag
     3301 gcaggtactg ctatgaacat cagcgttccc caggtgcagc tgatcaaccc agagaaccaa
     3361 attgtggagc cggatgggac actgaatttg gacagtgatg agggtgaaga gccttcccct
     3421 gaagcattgg tgcgctattt gtcaatgagg aggcacacag tgggtgtggc tgacccacgc
     3481 acggaagtta tggaagatct gcagaagctc ctacctggct ttcctggagt caacccccag
     3541 gctccattcc tgcaggtggc ccctaatgtg aacttcatgc acaacctgtt gcctatgcaa
     3601 aacttgcaac caaccgggca acttgagtac aaggagcagt ctctcctaca gccgcccacg
     3661 ctacagctgt tgaatggaat gggccccctt ggccggaggg catcagatgg aggagccaac
     3721 atccaactgc atgcccagca gctgctgaag cgcccacggg gaccctctcc gcttgtcacc
     3781 atgacaccag cagtgccagc agttacccct gtggacgagg agagctcaga cggggagcca
     3841 gaccaggaag ctgtgcagag gtacttggca aataggtcca aaagacatac actggccatg
     3901 accaacccta cagctgagat cccaccggac ctacaacggc agctaggaca gcagcctttc
     3961 cgttcccggg tctggcctcc tcacctggta cctgatcagc atcgctctac ctacaaggac
     4021 tccaacactc tgcacctccc tacggagcgt ttctcccctg tgcgccggtt ctcagatggg
     4081 gctgcgagca tccaggcctt caaagctcac ctggaaaaaa tgggcaacaa cagcagcatc
     4141 aaacagctgc agcaggagtg tgagcagctg cagaagatgt acggggggca gattgatgaa
     4201 agaaccctgg agaagaccca gcagcagcat atgttatacc agcaggagca gcaccatcaa
     4261 attctccagc aacaaattca agactctatc tgtcctcctc agccatctcc acctcttcag
     4321 gctgcatgtg aaaatcagcc agccctcctt acccatcagc tccagaggtt aaggattcag
     4381 ccttcaagcc caccccccaa ccaccccaac aaccatctct tcaggcagcc cagtaatagt
     4441 cctcccccca tgagcagtgc catgatccag cctcacgggg ctgcatcttc ttcccagttt
     4501 caaggcttac cttcccgcag tgcaatcttt cagcagcaac ctgagaactg ttcctctcct
     4561 cccaacgtgg cactaacctg cttgggtatg cagcagcctg ctcagtcaca gcaggtcacc
     4621 atccaagtcc aagagcctgt tgacatgctc agcaacatgc caggcacagc tgcaggctcc
     4681 agtgggcgcg gcatctccat cagccccagt gctggtcaga tgcagatgca gcaccgtacc
     4741 aacctgatgg ccaccctcag ctatgggcac cgtcccttgt ccaagcagct gagtgctgac
     4801 agtgcagagg ctcacagtgc acatcagcag ccgccacact ataccacgtc ggcactacag
     4861 caggccctgc tgtctcccac gccgccagac tatacaagac accagcaggt accccacatc
     4921 cttcaaggac tgctttctcc ccggcattcg ctcaccggcc actcggacat ccggctgccc
     4981 ccaacagagt ttgcacagct cattaaaagg cagcagcaac aacggcagca gcagcagcaa
     5041 cagcagcaac agcaagaata ccaggaactg ttcaggcaca tgaaccaagg ggatgcgggg
     5101 agtctggctc ccagccttgg gggacagagc atgacagagc gccaggcttt atcttatcaa
     5161 aatgctgact cttatcacca tcacaccagc ccccagcatc tgctacaaat cagggcacaa
     5221 gaatgtgtct cacaggcttc ctcacccacc ccgccccacg ggtatgctca ccagccggca
     5281 ctgatgcatt cagagagcat ggaggaggac tgctcgtgtg agggggccaa ggatggcttc
     5341 caagacagta agagttcaag tacattgacc aaaggttgcc atgacagccc tctgctcttg
     5401 agtaccggtg gacctgggga ccctgaatct ttgctaggaa ctgtgagtca tgcccaagaa
     5461 ttggggatac atccctatgg tcatcagcca actgctgcat tcagtaaaaa taaggtgccc
     5521 agcagagagc ctgtcatagg gaactgcatg gatagaagtt ctccaggaca agcagtggag
     5581 ctgccggatc acaatgggct cgggtaccca gcacgcccct ccgtccatga gcaccacagg
     5641 ccccgggccc tccagagaca ccacacgatc cagaacagcg acgatgctta tgtacagctg
     5701 gataacttgc caggaatgag tctcgtggct gggaaagcac ttagctctgc ccggatgtcg
     5761 gatgcagttc tcagtcagtc ttcgctcatg ggcagccagc agtttcagga tggggaaaat
     5821 gaggaatgtg gggcaagcct gggaggtcat gagcacccag acctgagtga tggcagccag
     5881 catttaaact cctcttgcta tccatctacg tgtattacag acattctgct cagctacaag
     5941 caccccgaag tctccttcag catggagcag gcaggcgtgg gcagcggcgc caccaacttc
     6001 agcctgctga agcaggccgg cgacgtggag gagaaccccg gccccatgac cgagtacaag
     6061 cccatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     6121 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     6181 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     6241 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     6301 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     6361 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     6421 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     6481 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     6541 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     6601 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     6661 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     6721 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     6781 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     6841 cttgacgagt tcttctgaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     6901 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6961 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     7021 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     7081 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7141 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7201 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7261 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     7321 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7381 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7441 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     7501 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     7561 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     7621 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7681 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7741 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     7801 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     7861 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7921 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     7981 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8041 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8101 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     8161 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     8221 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     8281 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     8341 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8401 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     8461 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8521 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     8581 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8641 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     8701 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     8761 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     8821 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     8881 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     8941 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     9001 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     9061 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     9121 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     9181 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     9241 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     9301 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     9361 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     9421 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     9481 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9541 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9601 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     9661 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     9721 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9781 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9841 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9901 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9961 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    10021 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    10081 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    10141 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10201 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10261 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    10321 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    10381 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    10441 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10501 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10561 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10621 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    10681 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    10741 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    10801 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    10861 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    10921 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
    10981 accctcact
//
