LOCUS       sequence-207841-       10989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant 
            silent mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Tarumoto Y, Lu B, Somerville TDD, Huang YH, Milazzo JP, Wu XS, 
            Klingbeil O, El Demerdash O, Shi J, Vakoc CR
  TITLE     LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in 
            Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011.
  PUBMED    29526696
REFERENCE   2  (bases 1 to 10989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011."
COMMENT     Sequence Label: LentiV_Neo_SIK3_CR_T221E
FEATURES             Location/Qualifiers
     source          1..10989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        17..243
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             244..424
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    471..596
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1089..1322
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1507..1551
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1735..1852
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1921..2132
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5973..6029
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6048..6842
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6102..6121)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6712..6731
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6858..7446
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6911..6931)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7329..7340)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7518..7751
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7829..7950
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7866..7885)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7920..7939
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7990..8125
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8145..8164)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8146..8164)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8174..8191)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8174..8190)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8183..8205)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8332..8787
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8419..8438)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8629..8650
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8813..8917
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8918..8986,8987..9778)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9136..9155)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9949..10537
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10438..10457
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10691..10708
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10825..10846
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10861..10866,10867..10884,10885..10891)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10899..10915
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10904..10926
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10923..10939
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10923..10939
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10958..10978
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10960..10978
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
       61 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
      121 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
      181 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
      241 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      301 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      361 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      421 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      481 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      541 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      601 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
      661 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
      721 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
      781 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
      841 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
      901 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
      961 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1021 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1081 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1141 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1201 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1261 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1321 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1381 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1441 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1501 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1561 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1621 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     1681 tcaccattat cgtttcagac ccacctccca accccgaggg gacccagacc acaattttaa
     1741 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     1801 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     1861 ttacagggac agcagagatc cactttggcg ccggctcgag tggctccggt gcccgtcagt
     1921 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     1981 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2041 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2101 tttttcgcaa cgggtttgcc gccagaacac aggtgtcgtg acgcgggatc cgccacccgg
     2161 aggtttcact gcacaacaag atggcggcgg cggcggcgag cggagctggc ggggctgccg
     2221 gggccgggac tgggggagcc gggcccgcgg gccgcctgct gcctccgccc gcgccggggt
     2281 ccccagccgc ccccgctgcc gtgtcccctg cggccggcca gccgcgtccc ccagccccgg
     2341 cctcccgcgg acccatgccc gcccgtatcg gctactacga gatcgaccgc accatcggca
     2401 agggcaactt cgcggtggtc aagcgggcca cgcacctcgt caccaaggcc aaggttgcta
     2461 tcaagatcat agataagacc cagctggatg aagaaaactt gaagaagatt ttccgggaag
     2521 ttcaaattat gaagatgctt tgccaccccc atatcatcag gctctaccag gttatggaga
     2581 cagaacggat gatttatctg gtgacagaat atgctagtgg aggggaaata tttgaccacc
     2641 tggtggccca tggtagaatg gcagaaaagg aggcacgtcg caagttcaaa cagatcgtca
     2701 cagctgtcta tttttgtcac tgtcggaaca ttgttcatcg tgatttaaaa gctgaaaatt
     2761 tacttctgga tgccaatctg aatatcaaaa tagcagattt tggtttcagt aacctcttca
     2821 ctcctgggca gctgctgaag gagtggtgtg gcagccctcc ctatgctgca cctgaactct
     2881 ttgaaggaaa agaatatgat gggcccaaag tggacatctg gagccttgga gttgtcctct
     2941 acgtgcttgt gtgcggtgcc ctgccatttg atggaagcac actgcagaat ctgcgggccc
     3001 gcgtgctgag tggaaagttc cgcatcccat tttttatgtc cacagaatgt gagcatttga
     3061 tccgccatat gttggtgtta gatcccaata agcgcctctc catggagcag atctgcaagc
     3121 acaagtggat gaagctaggg gacgccgatc ccaactttga caggttaata gctgaatgcc
     3181 aacaactaaa ggaagaaaga caggtggacc ccctgaatga ggatgtcctc ttggccatgg
     3241 aggacatggg actggacaaa gaacagacac tgcaggcgga gcaggcaggt actgctatga
     3301 acatcagcgt tccccaggtg cagctgatca acccagagaa ccaaattgtg gagccggatg
     3361 ggacactgaa tttggacagt gatgagggtg aagagccttc ccctgaagca ttggtgcgct
     3421 atttgtcaat gaggaggcac acagtgggtg tggctgaccc acgcacggaa gttatggaag
     3481 atctgcagaa gctcctacct ggctttcctg gagtcaaccc ccaggctcca ttcctgcagg
     3541 tggcccctaa tgtgaacttc atgcacaacc tgttgcctat gcaaaacttg caaccaaccg
     3601 ggcaacttga gtacaaggag cagtctctcc tacagccgcc cacgctacag ctgttgaatg
     3661 gaatgggccc ccttggccgg agggcatcag atggaggagc caacatccaa ctgcatgccc
     3721 agcagctgct gaagcgccca cggggaccct ctccgcttgt caccatgaca ccagcagtgc
     3781 cagcagttac ccctgtggac gaggagagct cagacgggga gccagaccag gaagctgtgc
     3841 agaggtactt ggcaaatagg tccaaaagac atacactggc catgaccaac cctacagctg
     3901 agatcccacc ggacctacaa cggcagctag gacagcagcc tttccgttcc cgggtctggc
     3961 ctcctcacct ggtacctgat cagcatcgct ctacctacaa ggactccaac actctgcacc
     4021 tccctacgga gcgtttctcc cctgtgcgcc ggttctcaga tggggctgcg agcatccagg
     4081 ccttcaaagc tcacctggaa aaaatgggca acaacagcag catcaaacag ctgcagcagg
     4141 agtgtgagca gctgcagaag atgtacgggg ggcagattga tgaaagaacc ctggagaaga
     4201 cccagcagca gcatatgtta taccagcagg agcagcacca tcaaattctc cagcaacaaa
     4261 ttcaagactc tatctgtcct cctcagccat ctccacctct tcaggctgca tgtgaaaatc
     4321 agccagccct ccttacccat cagctccaga ggttaaggat tcagccttca agcccacccc
     4381 ccaaccaccc caacaaccat ctcttcaggc agcccagtaa tagtcctccc cccatgagca
     4441 gtgccatgat ccagcctcac ggggctgcat cttcttccca gtttcaaggc ttaccttccc
     4501 gcagtgcaat ctttcagcag caacctgaga actgttcctc tcctcccaac gtggcactaa
     4561 cctgcttggg tatgcagcag cctgctcagt cacagcaggt caccatccaa gtccaagagc
     4621 ctgttgacat gctcagcaac atgccaggca cagctgcagg ctccagtggg cgcggcatct
     4681 ccatcagccc cagtgctggt cagatgcaga tgcagcaccg taccaacctg atggccaccc
     4741 tcagctatgg gcaccgtccc ttgtccaagc agctgagtgc tgacagtgca gaggctcaca
     4801 gtgcacatca gcagccgcca cactatacca cgtcggcact acagcaggcc ctgctgtctc
     4861 ccacgccgcc agactataca agacaccagc aggtacccca catccttcaa ggactgcttt
     4921 ctccccggca ttcgctcacc ggccactcgg acatccggct gcccccaaca gagtttgcac
     4981 agctcattaa aaggcagcag caacaacggc agcagcagca gcaacagcag caacagcaag
     5041 aataccagga actgttcagg cacatgaacc aaggggatgc ggggagtctg gctcccagcc
     5101 ttgggggaca gagcatgaca gagcgccagg ctttatctta tcaaaatgct gactcttatc
     5161 accatcacac cagcccccag catctgctac aaatcagggc acaagaatgt gtctcacagg
     5221 cttcctcacc caccccgccc cacgggtatg ctcaccagcc ggcactgatg cattcagaga
     5281 gcatggagga ggactgctcg tgtgaggggg ccaaggatgg cttccaagac agtaagagtt
     5341 caagtacatt gaccaaaggt tgccatgaca gccctctgct cttgagtacc ggtggacctg
     5401 gggaccctga atctttgcta ggaactgtga gtcatgccca agaattgggg atacatccct
     5461 atggtcatca gccaactgct gcattcagta aaaataaggt gcccagcaga gagcctgtca
     5521 tagggaactg catggataga agttctccag gacaagcagt ggagctgccg gatcacaatg
     5581 ggctcgggta cccagcacgc ccctccgtcc atgagcacca caggccccgg gccctccaga
     5641 gacaccacac gatccagaac agcgacgatg cttatgtaca gctggataac ttgccaggaa
     5701 tgagtctcgt ggctgggaaa gcacttagct ctgcccggat gtcggatgca gttctcagtc
     5761 agtcttcgct catgggcagc cagcagtttc aggatgggga aaatgaggaa tgtggggcaa
     5821 gcctgggagg tcatgagcac ccagacctga gtgatggcag ccagcattta aactcctctt
     5881 gctatccatc tacgtgtatt acagacattc tgctcagcta caagcacccc gaagtctcct
     5941 tcagcatgga gcaggcaggc gtgggcagcg gcgccaccaa cttcagcctg ctgaagcagg
     6001 ccggcgacgt ggaggagaac cccggcccca tgaccgagta caagcccatg attgaacaag
     6061 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     6121 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     6181 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     6241 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     6301 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     6361 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     6421 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     6481 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     6541 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     6601 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     6661 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6721 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6781 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6841 gaacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     6901 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     6961 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     7021 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     7081 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     7141 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     7201 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     7261 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     7321 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     7381 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     7441 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7501 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     7561 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7621 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7681 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7741 aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc
     7801 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     7861 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7921 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     7981 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8041 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8101 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     8161 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     8221 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     8281 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     8341 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     8401 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     8461 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     8521 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     8581 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     8641 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     8701 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     8761 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     8821 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     8881 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     8941 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     9001 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     9061 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     9121 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     9181 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     9241 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     9301 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     9361 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     9421 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     9481 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     9541 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     9601 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     9661 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     9721 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     9781 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     9841 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     9901 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     9961 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    10021 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    10081 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    10141 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    10201 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    10261 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    10321 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    10381 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    10441 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    10501 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    10561 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    10621 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
    10681 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
    10741 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
    10801 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
    10861 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
    10921 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
    10981 acaaaagct
//
