LOCUS       sequence-207842-       10989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant 
            silent mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Tarumoto Y, Lu B, Somerville TDD, Huang YH, Milazzo JP, Wu XS, 
            Klingbeil O, El Demerdash O, Shi J, Vakoc CR
  TITLE     LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in 
            Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011.
  PUBMED    29526696
REFERENCE   2  (bases 1 to 10989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011."
COMMENT     Sequence Label: LentiV_Neo_SIK3_CR_T142Q
FEATURES             Location/Qualifiers
     source          1..10989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        28..254
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             255..435
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    482..607
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1100..1333
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1518..1562
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1746..1863
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1932..2143
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5984..6040
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6059..6853
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6113..6132)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6723..6742
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6869..7457
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6922..6942)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7340..7351)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7529..7762
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7840..7961
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7877..7896)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7931..7950
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8001..8136
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8156..8175)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8157..8175)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8185..8202)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8185..8201)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8194..8216)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8343..8798
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8430..8449)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8640..8661
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8824..8928
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8929..8997,8998..9789)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9147..9166)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9960..10548
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10449..10468
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10702..10719
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10836..10857
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10872..10877,10878..10895,10896..10902)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10910..10926
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10915..10937
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10934..10950
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10934..10950
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10969..10989
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10971..10989
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
       61 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
      121 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
      181 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
      241 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
      301 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
      361 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
      421 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
      481 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
      541 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
      601 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
      661 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
      721 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
      781 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
      841 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
      901 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
      961 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     1021 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     1081 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     1141 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     1201 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     1261 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     1321 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     1381 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     1441 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     1501 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     1561 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     1621 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     1681 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccagac
     1741 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     1801 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     1861 tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga gtggctccgg
     1921 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     1981 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     2041 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     2101 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtgtcgt gacgcgggat
     2161 ccgccacccg gaggtttcac tgcacaacaa gatggcggcg gcggcggcga gcggagctgg
     2221 cggggctgcc ggggccggga ctgggggagc cgggcccgcg ggccgcctgc tgcctccgcc
     2281 cgcgccgggg tccccagccg cccccgctgc cgtgtcccct gcggccggcc agccgcgtcc
     2341 cccagccccg gcctcccgcg gacccatgcc cgcccgtatc ggctactacg agatcgaccg
     2401 caccatcggc aagggcaact tcgcggtggt caagcgggcc acgcacctcg tcaccaaggc
     2461 caaggttgct atcaagatca tagataagac ccagctggat gaagaaaact tgaagaagat
     2521 tttccgggaa gttcaaatta tgaagatgct ttgccacccc catatcatca ggctctacca
     2581 ggttatggag acagaacgga tgatttatct ggtgcaagaa tatgctagtg gaggggaaat
     2641 atttgaccac ctggtggccc atggtagaat ggcagaaaag gaggcacgtc gcaagttcaa
     2701 acagatcgtc acagctgtct atttttgtca ctgtcggaac attgttcatc gtgatttaaa
     2761 agctgaaaat ttacttctgg atgccaatct gaatatcaaa atagcagatt ttggtttcag
     2821 taacctcttc actcctgggc agctgctgaa gacctggtgt ggcagccctc cctatgctgc
     2881 acctgaactc tttgaaggaa aagaatatga tgggcccaaa gtggacatct ggagccttgg
     2941 agttgtcctc tacgtgcttg tgtgcggtgc cctgccattt gatggaagca cactgcagaa
     3001 tctgcgggcc cgcgtgctga gtggaaagtt ccgcatccca ttttttatgt ccacagaatg
     3061 tgagcatttg atccgccata tgttggtgtt agatcccaat aagcgcctct ccatggagca
     3121 gatctgcaag cacaagtgga tgaagctagg ggacgccgat cccaactttg acaggttaat
     3181 agctgaatgc caacaactaa aggaagaaag acaggtggac cccctgaatg aggatgtcct
     3241 cttggccatg gaggacatgg gactggacaa agaacagaca ctgcaggcgg agcaggcagg
     3301 tactgctatg aacatcagcg ttccccaggt gcagctgatc aacccagaga accaaattgt
     3361 ggagccggat gggacactga atttggacag tgatgagggt gaagagcctt cccctgaagc
     3421 attggtgcgc tatttgtcaa tgaggaggca cacagtgggt gtggctgacc cacgcacgga
     3481 agttatggaa gatctgcaga agctcctacc tggctttcct ggagtcaacc cccaggctcc
     3541 attcctgcag gtggccccta atgtgaactt catgcacaac ctgttgccta tgcaaaactt
     3601 gcaaccaacc gggcaacttg agtacaagga gcagtctctc ctacagccgc ccacgctaca
     3661 gctgttgaat ggaatgggcc cccttggccg gagggcatca gatggaggag ccaacatcca
     3721 actgcatgcc cagcagctgc tgaagcgccc acggggaccc tctccgcttg tcaccatgac
     3781 accagcagtg ccagcagtta cccctgtgga cgaggagagc tcagacgggg agccagacca
     3841 ggaagctgtg cagaggtact tggcaaatag gtccaaaaga catacactgg ccatgaccaa
     3901 ccctacagct gagatcccac cggacctaca acggcagcta ggacagcagc ctttccgttc
     3961 ccgggtctgg cctcctcacc tggtacctga tcagcatcgc tctacctaca aggactccaa
     4021 cactctgcac ctccctacgg agcgtttctc ccctgtgcgc cggttctcag atggggctgc
     4081 gagcatccag gccttcaaag ctcacctgga aaaaatgggc aacaacagca gcatcaaaca
     4141 gctgcagcag gagtgtgagc agctgcagaa gatgtacggg gggcagattg atgaaagaac
     4201 cctggagaag acccagcagc agcatatgtt ataccagcag gagcagcacc atcaaattct
     4261 ccagcaacaa attcaagact ctatctgtcc tcctcagcca tctccacctc ttcaggctgc
     4321 atgtgaaaat cagccagccc tccttaccca tcagctccag aggttaagga ttcagccttc
     4381 aagcccaccc cccaaccacc ccaacaacca tctcttcagg cagcccagta atagtcctcc
     4441 ccccatgagc agtgccatga tccagcctca cggggctgca tcttcttccc agtttcaagg
     4501 cttaccttcc cgcagtgcaa tctttcagca gcaacctgag aactgttcct ctcctcccaa
     4561 cgtggcacta acctgcttgg gtatgcagca gcctgctcag tcacagcagg tcaccatcca
     4621 agtccaagag cctgttgaca tgctcagcaa catgccaggc acagctgcag gctccagtgg
     4681 gcgcggcatc tccatcagcc ccagtgctgg tcagatgcag atgcagcacc gtaccaacct
     4741 gatggccacc ctcagctatg ggcaccgtcc cttgtccaag cagctgagtg ctgacagtgc
     4801 agaggctcac agtgcacatc agcagccgcc acactatacc acgtcggcac tacagcaggc
     4861 cctgctgtct cccacgccgc cagactatac aagacaccag caggtacccc acatccttca
     4921 aggactgctt tctccccggc attcgctcac cggccactcg gacatccggc tgcccccaac
     4981 agagtttgca cagctcatta aaaggcagca gcaacaacgg cagcagcagc agcaacagca
     5041 gcaacagcaa gaataccagg aactgttcag gcacatgaac caaggggatg cggggagtct
     5101 ggctcccagc cttgggggac agagcatgac agagcgccag gctttatctt atcaaaatgc
     5161 tgactcttat caccatcaca ccagccccca gcatctgcta caaatcaggg cacaagaatg
     5221 tgtctcacag gcttcctcac ccaccccgcc ccacgggtat gctcaccagc cggcactgat
     5281 gcattcagag agcatggagg aggactgctc gtgtgagggg gccaaggatg gcttccaaga
     5341 cagtaagagt tcaagtacat tgaccaaagg ttgccatgac agccctctgc tcttgagtac
     5401 cggtggacct ggggaccctg aatctttgct aggaactgtg agtcatgccc aagaattggg
     5461 gatacatccc tatggtcatc agccaactgc tgcattcagt aaaaataagg tgcccagcag
     5521 agagcctgtc atagggaact gcatggatag aagttctcca ggacaagcag tggagctgcc
     5581 ggatcacaat gggctcgggt acccagcacg cccctccgtc catgagcacc acaggccccg
     5641 ggccctccag agacaccaca cgatccagaa cagcgacgat gcttatgtac agctggataa
     5701 cttgccagga atgagtctcg tggctgggaa agcacttagc tctgcccgga tgtcggatgc
     5761 agttctcagt cagtcttcgc tcatgggcag ccagcagttt caggatgggg aaaatgagga
     5821 atgtggggca agcctgggag gtcatgagca cccagacctg agtgatggca gccagcattt
     5881 aaactcctct tgctatccat ctacgtgtat tacagacatt ctgctcagct acaagcaccc
     5941 cgaagtctcc ttcagcatgg agcaggcagg cgtgggcagc ggcgccacca acttcagcct
     6001 gctgaagcag gccggcgacg tggaggagaa ccccggcccc atgaccgagt acaagcccat
     6061 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     6121 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     6181 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     6241 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     6301 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     6361 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     6421 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     6481 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     6541 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgatgg
     6601 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     6661 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     6721 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     6781 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     6841 cgagttcttc tgaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     6901 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6961 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     7021 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     7081 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     7141 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     7201 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     7261 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     7321 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     7381 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     7441 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     7501 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     7561 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7621 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7681 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7741 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
     7801 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     7861 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7921 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     7981 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     8041 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8101 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     8161 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     8221 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     8281 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     8341 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     8401 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     8461 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     8521 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     8581 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     8641 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     8701 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     8761 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     8821 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     8881 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     8941 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     9001 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     9061 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     9121 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     9181 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     9241 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     9301 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     9361 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     9421 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     9481 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     9541 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     9601 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     9661 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     9721 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     9781 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     9841 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     9901 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     9961 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    10021 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    10081 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    10141 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    10201 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    10261 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    10321 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    10381 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    10441 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    10501 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    10561 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    10621 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    10681 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    10741 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
    10801 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    10861 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
    10921 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
    10981 cactaaagg
//
