LOCUS       sequence-207844-        8557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of human LKB1 cDNA with neomycin 
            resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Tarumoto Y, Lu B, Somerville TDD, Huang YH, Milazzo JP, Wu XS, 
            Klingbeil O, El Demerdash O, Shi J, Vakoc CR
  TITLE     LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in 
            Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011.
  PUBMED    29526696
REFERENCE   2  (bases 1 to 8557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011."
COMMENT     Sequence Label: LentiV_Neo_LKB1
FEATURES             Location/Qualifiers
     source          1..8557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             219..399
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    446..571
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1064..1297
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1482..1526
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1710..1827
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1896..2107
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2127..2136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2136..2159
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3516..3572
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3591..4385
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3645..3664)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4255..4274
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    4401..4989
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4454..4474)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4872..4883)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5061..5294
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5372..5493
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5409..5428)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5463..5482
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5533..5668
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5688..5707)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5689..5707)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5717..5734)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5717..5733)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5726..5748)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5875..6330
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5962..5981)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6172..6193
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6356..6460
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6461..6529,6530..7321)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6679..6698)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7492..8080
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7981..8000
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8234..8251
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8368..8389
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8404..8409,8410..8427,8428..8434)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8442..8458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8447..8469
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8466..8482
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8466..8482
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8501..8521
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8503..8521
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        join(8549..8557,1..218)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
       61 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
      121 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
      181 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
      241 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
      301 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
      361 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
      421 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
      481 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
      541 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
      601 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
      661 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
      721 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
      781 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
      841 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
      901 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
      961 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1021 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1081 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1141 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1201 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1261 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     1321 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     1381 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     1441 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     1501 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     1561 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     1621 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     1681 tcccaacccc gaggggaccc agaccacaat tttaaaagaa aaggggggat tggggggtac
     1741 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     1801 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
     1861 tggcgccggc tcgagtggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     1921 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
     1981 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     2041 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     2101 aacacaggtg tcgtgacgcg ggatccgcca ccatggacta caaagacgat gacgataaag
     2161 caagggatcc actagtaacg gccgccagtg tgctggaatt ctccagcatg gaggtggtgg
     2221 acccgcagca gctgggcatg ttcacggagg gcgagctgat gtcggtgggt atggacacgt
     2281 tcatccaccg catcgactcc accgaggtca tctaccagcc gcgccgcaag cgggccaagc
     2341 tcatcggcaa gtacctgatg ggggacctgc tgggggaagg ctcttacggc aaggtgaagg
     2401 aggtgctgga ctcggagacg ctgtgcagga gggccgtcaa gatcctcaag aagaagaagt
     2461 tgcgaaggat ccccaacggg gaggccaacg tgaagaagga aattcaacta ctgaggaggt
     2521 tacggcacaa aaatgtcatc cagctggtgg atgtgttata caacgaagag aagcagaaaa
     2581 tgtatatggt gatggagtac tgcgtgtgtg gcatgcagga aatgctggac agcgtgccgg
     2641 agaagcgttt cccagtgtgc caggcccacg ggtacttctg tcagctgatt gacggcctgg
     2701 agtacctgca tagccagggc attgtgcaca aggacatcaa gccggggaac ctgctgctca
     2761 ccaccggtgg caccctcaaa atctccgacc tgggcgtggc cgaggcactg cacccgttcg
     2821 cggcggacga cacctgccgg accagccagg gctccccggc tttccagccg cccgagattg
     2881 ccaacggcct ggacaccttc tccggcttca aggtggacat ctggtcggct ggggtcaccc
     2941 tctacaacat caccacgggt ctgtacccct tcgaagggga caacatctac aagttgtttg
     3001 agaacatcgg gaaggggagc tacgccatcc cgggcgactg tggccccccg ctctctgacc
     3061 tgctgaaagg gatgcttgag tacgaaccgg ccaagaggtt ctccatccgg cagatccggc
     3121 agcacagctg gttccggaag aaacatcctc cggctgaagc accagtgccc atcccaccga
     3181 gcccagacac caaggaccgg tggcgcagca tgactgtggt gccgtacttg gaggacctgc
     3241 acggcgcgga cgaggacgag gacctcttcg acatcgagga tgacatcatc tacactcagg
     3301 acttcacggt gcccggacag gtcccagaag aggaggccag tcacaatgga cagcgccggg
     3361 gcctccccaa ggccgtgtgt atgaacggca cagaggcggc gcagctgagc accaaatcca
     3421 gggcggaggg ccgggccccc aaccctgccc gcaaggcctg ctccgccagc agcaagatcc
     3481 gccggctgtc ggcctgcaag cagcagggca gcggcgccac caacttcagc ctgctgaagc
     3541 aggccggcga cgtggaggag aaccccggcc ccatgaccga gtacaagccc atgattgaac
     3601 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     3661 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     3721 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     3781 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     3841 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     3901 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     3961 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     4021 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     4081 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     4141 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     4201 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     4261 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     4321 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     4381 tctgaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     4441 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4501 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4561 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4621 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4681 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4741 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4801 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4861 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4921 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4981 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     5041 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     5101 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5161 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5221 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5281 gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt atttataact
     5341 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     5401 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5461 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     5521 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5581 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     5641 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     5701 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     5761 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     5821 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     5881 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5941 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     6001 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6061 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     6121 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6181 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6241 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6301 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     6361 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6421 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     6481 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6541 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     6601 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     6661 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6721 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6781 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6841 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6901 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6961 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7021 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7081 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7141 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7201 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7261 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7321 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7381 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7441 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7501 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7561 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7621 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7681 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7741 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7801 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7861 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7921 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7981 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8041 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8101 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8161 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8221 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     8281 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     8341 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     8401 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     8461 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     8521 ggaacaaaag ctggagctgc aagcttaatg tagtctt
//
