LOCUS       sequence-207845-        8557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of human LKB1 cDNA (CRISPR-resistant 
            silent mutation) with neomycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Tarumoto Y, Lu B, Somerville TDD, Huang YH, Milazzo JP, Wu XS, 
            Klingbeil O, El Demerdash O, Shi J, Vakoc CR
  TITLE     LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in 
            Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011.
  PUBMED    29526696
REFERENCE   2  (bases 1 to 8557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011."
COMMENT     Sequence Label: LentiV_Neo_LKB1_CR
FEATURES             Location/Qualifiers
     source          1..8557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..233
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    461..586
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1079..1312
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1497..1541
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1725..1842
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1911..2122
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2142..2151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2151..2174
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3531..3587
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3606..4400
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3660..3679)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4270..4289
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    4416..5004
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4469..4489)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4887..4898)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5076..5309
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5387..5508
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5424..5443)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5478..5497
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5548..5683
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5703..5722)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5704..5722)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5732..5749)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5732..5748)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5741..5763)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5890..6345
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5977..5996)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6187..6208
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6371..6475
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6476..6544,6545..7336)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6694..6713)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7507..8095
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7996..8015
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8249..8266
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8383..8404
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8419..8424,8425..8442,8443..8449)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8457..8473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8462..8484
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8481..8497
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8481..8497
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8516..8536
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8518..8536
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
       61 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
      121 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
      181 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
      481 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
      541 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
      601 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
      661 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
      721 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
      781 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
      841 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
      901 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
      961 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1021 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1081 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1141 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1201 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1261 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1321 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1381 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     1441 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     1501 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     1561 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     1621 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     1681 cgtttcagac ccacctccca accccgaggg gacccagacc acaattttaa aagaaaaggg
     1741 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     1801 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     1861 agcagagatc cactttggcg ccggctcgag tggctccggt gcccgtcagt gggcagagcg
     1921 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta
     1981 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     2041 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     2101 cgggtttgcc gccagaacac aggtgtcgtg acgcgggatc cgccaccatg gactacaaag
     2161 acgatgacga taaagcaagg gatccactag taacggccgc cagtgtgctg gaattctcca
     2221 gcatggaggt ggtggacccg cagcagctgg gcatgttcac ggagggcgag ctgatgtcgg
     2281 tgggtatgga cacgttcatc caccgcatcg actccaccga ggtcatctac cagccgcgcc
     2341 gcaagcgggc caagctcatc ggcaagtacc tgatggggga cctgctgggg gaaggctctt
     2401 acggcaaggt gaaggaggtg ctggactcgg agacgctgtg caggagggcc gtcaagatcc
     2461 tcaagaagaa gaagttgcga aggatcccca acggggaggc caacgtgaag aaggaaattc
     2521 aactactgag gaggttacgg cacaaaaatg tcatacagct ggtggatgtg ttatacaacg
     2581 aagagaagca gaaaatgtat atggtgatgg agtactgcgt gtgtggcatg caggaaatgc
     2641 tggacagcgt gccggagaag cgtttcccag tgtgccaggc ccacgggtac ttctgtcagc
     2701 tgattgacgg cctggagtac ctgcatagcc agggcattgt gcacaaggac atcaagccgg
     2761 ggaacctgct gctcaccacc ggtggcaccc tcaaaatctc cgacctgggc gtggccgagg
     2821 cactgcaccc gttcgcggcg gacgacacct gccggaccag ccagggctcc ccggctttcc
     2881 agccgcccga gattgccaac ggcctggaca ccttctccgg cttcaaggtg gacatctggt
     2941 cggctggggt caccctctac aacatcacca cgggtctgta ccccttcgaa ggggacaaca
     3001 tctacaagtt gtttgagaac atcgggaagg ggagctacgc catcccgggc gactgtggcc
     3061 ccccgctctc tgacctgctg aaagggatgc ttgagtacga accggccaag aggttctcca
     3121 tccggcagat ccggcagcac agctggttcc ggaagaaaca tcctccggct gaagcaccag
     3181 tgcccatccc accgagccca gacaccaagg accggtggcg cagcatgact gtggtgccgt
     3241 acttggagga cctgcacggc gcggacgagg acgaggacct cttcgacatc gaggatgaca
     3301 tcatctacac tcaggacttc acggtgcccg gacaggtccc agaagaggag gccagtcaca
     3361 atggacagcg ccggggcctc cccaaggccg tgtgtatgaa cggcacagag gcggcgcagc
     3421 tgagcaccaa atccagggcg gagggccggg cccccaaccc tgcccgcaag gcctgctccg
     3481 ccagcagcaa gatccgccgg ctgtcggcct gcaagcagca gggcagcggc gccaccaact
     3541 tcagcctgct gaagcaggcc ggcgacgtgg aggagaaccc cggccccatg accgagtaca
     3601 agcccatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     3661 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     3721 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     3781 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     3841 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     3901 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     3961 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     4021 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     4081 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     4141 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     4201 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     4261 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     4321 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     4381 ttcttgacga gttcttctga acgcgttaag tcgacaatca acctctggat tacaaaattt
     4441 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4501 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4561 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4621 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4681 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4741 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4801 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     4861 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4921 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4981 tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt
     5041 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     5101 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5161 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5221 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5281 cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat
     5341 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     5401 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5461 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     5521 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5581 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5641 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     5701 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     5761 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     5821 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     5881 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     5941 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6001 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6061 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6121 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6181 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     6241 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     6301 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     6361 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6421 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     6481 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6541 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6601 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6661 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     6721 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     6781 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     6841 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     6901 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6961 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7021 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7081 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7141 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7201 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7261 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7321 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     7381 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     7441 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7501 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7561 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7621 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     7681 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7741 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7801 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7861 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7921 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7981 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8041 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8101 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8161 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8221 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     8281 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     8341 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     8401 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     8461 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     8521 taaccctcac taaagggaac aaaagctgga gctgcaa
//
