LOCUS       sequence-20785-e         538 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG426GPD-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..538
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(83..207)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             266..343
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             371..391
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
       61 cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt
      121 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
      181 tcgttcagct ttcttgtaca aagtggtgat gggctgcagg aattcgatat caagcttatc
      241 gataggtgga ccaggtggtg gaatgaagcg acgatggaaa aagaatttca tagccgtctc
      301 agcagccaac cgctttaaga aaatctcatc ctccggggca cttgattatg gtaccccaac
      361 tactgcttct gaaaatctat attttcaagg tgaactaaaa actgctgctt tggctcaaca
      421 tgcgattaag ggtgaagctc anaaacttaa tgactctcaa gctccaaaag ctgatgcgca
      481 acaaaataac ttcaacaaag atcaacaaag cgccttctat gaaatcttga acatgcct
//
