LOCUS       sequence-207860-        8621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabinose-inducible split T7*RNAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Boehm CR, Grant PK, Haseloff J
  TITLE     Programmed hierarchical patterning of bacterial populations.
  JOURNAL   Nat Commun. 2018 Feb 22;9(1):776. doi: 10.1038/s41467-018-03069-3.
  PUBMED    29472537
REFERENCE   2  (bases 1 to 8621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-02-22"; volume: "9(1):776. doi"; pages: " 
            10.1038/s41467-018-03069-3"
COMMENT     Sequence Label: pTSara*
FEATURES             Location/Qualifiers
     source          1..8621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(96..974)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     terminator      complement(1218..1245)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(4655..4672)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4825..4911
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5003..5030
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5049..5140
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5598..6053
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5685..5704)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5895..5916
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6609..7268)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7183..7202
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(7269..7371)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(7763..7780)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7897..8442)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7977..7996)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8518..8537
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aatttcaaca cgagccaaaa taataaaaaa gcaggattaa taatctggct ttttatattc
     1201 tctctctagt atataaacgc agaaaggccc acccgaaggt gagccagtgt gatccaagcc
     1261 agctcgatat ccgtcggctt gaacgaattg ttagacatta cttgtagacg tgccctacgc
     1321 gcttgttgag ttgttcctca acgtttttct tgaagtgctt agcttcaagg tcacggatac
     1381 gaccgaagcg agcctcgtcc tcaatggccc gaccgattgc gcttgctaca gcctgaacgg
     1441 ttgtattgtc agcactggtt aggcaagcca gagtggtctt aatggtgatg tacgctacgg
     1501 cttccggctt gatttcttgc aggaactgga aggctgtcgg gcgcttgccg cgcttagctt
     1561 tcacttcctc aaaccagtcg ttgatgcgtg caatcatctt agggagtagg gtagtgatga
     1621 gaggcttggc ggcagcgtta tccgcaacct caccagcttt aagttgacgc tcaaacatct
     1681 tgcggaagcg tgcttcaccc atctcgtaag actcatgctc aagggccaac tgttcgcgag
     1741 ctaaacgctc accgtaatgg tcagccagag tgttgaacgg gatagcagcc agttcgatgt
     1801 cagagaagtc gttcttagcg atgttaatcg tgttcatatt agaaaaagaa tgttattaag
     1861 taagtctttc tagagctcgc tagcccaaaa aaacgggtat ggagaaacag tagagagttg
     1921 cgataaaaag cgtcaggtag gatccgctaa tcttatggat aaaaatgcta tggcatagca
     1981 aagtgtgacg ccgtgcaaat aatcaatgtc ctaggacttt tctgccgtga ttatagacac
     2041 ttttgtcgcg atgttgtgtc taattttgaa gttaactttg attccattct tttgtttgtc
     2101 tgccatgatg tatacattgt gtgagttata gttgtattcc aatttgtgtc caagaatgtt
     2161 tccatcttct ttaaaatcaa taccttttaa ctcgattcta ttaacaaggg tatcaccttc
     2221 aaacttgact tcagcacgtg tcttgtagtt cccgtcatct ttgaaaaata tagttctttc
     2281 ctgtacataa ccttcgggca ctcgagacat tgattatttg cacggcgtca cactttgcta
     2341 tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact
     2401 ctctactgtt tctccatacc cgtttttttg ggctagcact agtggagccc tatatataaa
     2461 gatcagggcc aggtatgaaa gcatttatgc aagttgtcga ggctgacatg ctctctaagg
     2521 gtctactcgg tggcgaggcg tggtcttcgt ggcataagga agactctatt catgtaggag
     2581 tacgctgcat cgagatgctc attgagtcaa ccggaatggt tagcttacac cgccaaaatg
     2641 ctggcgtagt aggtcaagac tctgagacta tcgaactcgc acctgaatac gctgaggcta
     2701 tcgcaacccg tgcaggtgcg ctggctggca tctctccgat gttccaacct tgcgtagttc
     2761 ctcctaagcc gtggactggc attactggtg gtggctattg ggctaacggt cgtcgtcctc
     2821 tggcgctggt gcgtactcac agtaagaaag cactgatgcg ctacgaagac gtttacatgc
     2881 ctgaggtgta caaagcgatt aacattgcgc aaaacaccgc atggaaaatc aacaagaaag
     2941 tcctagcggt cgccaacgta atcaccaagt ggaagcattg tccggtcgag gacatccctg
     3001 cgattgagcg tgaagaactc ccgatgaaac cggaagacat cgacatgaat cctgaggctc
     3061 tcaccgcgtg gaaacgtgct gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc
     3121 gccgtatcag ccttgagttc atgcttgagc aagccaataa gtttgctaac cataaggcca
     3181 tctggttccc ttacaacatg gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc
     3241 cgcaaggtaa cgatatgacc aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta
     3301 aggaaggtta ctactggctg aaaatccacg gtgcaaactg tgcgggtgtc gataaggttc
     3361 cgttccctga gcgcatcaag ttcattgagg aaaaccacga gaacatcatg gcttgcgcta
     3421 agtctccact ggagaacact tggtgggctg agcaagattc tccgttctgc ttccttgcgt
     3481 tctgctttga gtacgctggg gtacagcacc acggcctgag ctataactgc tcccttccgc
     3541 tggcgtttga cgggtcttgc tctggcatcc agcacttctc cgcgatgctc cgagatgagg
     3601 taggtggtcg cgcggttaac ttgcttccta gtgaaaccgt tcaggacatc tacgggattg
     3661 ttgctaagaa agtcaacgag attctacaag cagacgcaat caatgggacc gataacgaag
     3721 tagttaccgt gaccgatgag aacactggtg aaatctctga gaaagtcaag ctgggcacta
     3781 aggcactggc tggtcaatgg ctggcttacg gtgttactcg cagtgtgact aagagttcag
     3841 tcatgacgct ggcttacggg tccaaagagt tcggcttccg tcaacaagtg ctggaagata
     3901 ccattcagcc agctattgat tccggcaagg gtctgatgtt cactcagccg aatcaggctg
     3961 ctggatacat ggctaagctg atttgggaat ctgtgagcgt gacggtggta gctgcggttg
     4021 aagcaatgaa ctggcttaag tctgctgcta agctgctggc tgctgaggtc aaagataaga
     4081 agactggaga gattcttcgc aagcgttgcg ctgtgcattg ggtaactcct gatggtttcc
     4141 ctgtgtggca ggaatacaag aagcctattc agacgcgctt gaacctgatg ttcctcggtc
     4201 agttccgctt acagcctacc attaacacca acaaagatag cgagattgat gcacacaaac
     4261 aggagtctgg tatcgctcct aactttgtac acagccaaga cggtagccac cttcgtaaga
     4321 ctgtagtgtg ggcacacgag aagtacggaa tcgaatcttt tgcactgatt cacgactcct
     4381 tcggtaccat tccggctgac gctgcgaacc tgttcaaagc agtgcgcgaa actatggttg
     4441 acacatatga gtcttgtgat gtactggctg atttctacga ccagttcgct gaccagttgc
     4501 acgagtctca attggacaaa atgccagcac ttccggctaa aggtaacttg aacctccgtg
     4561 acatcttaga gtcggacttc gcgttcgcgt aatctagagt cgacctgcag gcatgcaagc
     4621 ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca
     4681 gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc
     4741 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
     4801 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     4861 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
     4921 gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
     4981 actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta
     5041 caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5101 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5161 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5221 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca gcaaactatt aactggcgaa
     5281 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5341 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5401 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5461 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5521 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5581 atactttaga ttgatttacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5641 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5701 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5761 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     5821 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5881 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
     5941 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     6001 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa tttaaaagga
     6061 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6121 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6181 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6241 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6301 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6361 cgcctacata cctcgctctg ctaatcctgt taccagtcag gcatttgaga agcacacggt
     6421 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6481 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6541 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     6601 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     6661 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     6721 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     6781 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     6841 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     6901 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     6961 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7021 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7081 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7141 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7201 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7261 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7321 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7381 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7441 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct
     7501 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc
     7561 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc
     7621 cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc
     7681 agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt
     7741 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc
     7801 ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac
     7861 ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga
     7921 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag
     7981 ataccaggcg tttccccctg gcggctccct cgtgcgctct cctgttcctg cctttcggtt
     8041 taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt
     8101 ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc
     8161 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac
     8221 cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc atgcgccggt
     8281 taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca gttacctcgg
     8341 ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg
     8401 ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaat
     8461 cagataaaat atttctaggc tcatgagccc gaagtggcga gcccgatctt ccccatcggt
     8521 gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat
     8581 gcgtccggcg tagaggatct gctcatgttt gacagcttat c
//
