LOCUS       sequence-207864-        1907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Monitor the expression of Luciferase driven by the NRAS 5'-UTR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Kumari S, Bugaut A, Huppert JL, Balasubramanian S
  TITLE     An RNA G-quadruplex in the 5' UTR of the NRAS proto-oncogene 
            modulates translation.
  JOURNAL   Nat Chem Biol. 2007 Apr;3(4):218-21. doi: 10.1038/nchembio864. Epub 
            2007 Feb 25.
  PUBMED    17322877
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio864"; journalName: "Nat Chem Biol"; date: 
            "2007-04"; volume: "3"; issue: "4"; pages: "218-21"
COMMENT     Sequence Label: pSKC11 - UTRQ
FEATURES             Location/Qualifiers
     source          1..1907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             255..1907
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(321..339)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gggacgtccc gtgtgggagg ggcgggtctg ggtgcggcct gccgcatgac tcgtggttcg
       61 gaggcccacg tggccggggc ggggactcag gcgcctgggg cgccgactga ttacgtagcg
      121 ggcggggccg gaagtgccgc tccttggtgg gggctgttca tggcggttcc ggggtctcca
      181 acatttttcc cggctgtggt cctaaatctg tccaaagcag aggcagtgga gcttgaggtt
      241 cttgctggtg tgaaatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc
      301 cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg
      361 ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt
      421 acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc
      481 acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt
      541 tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca
      601 acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa
      661 ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa
      721 cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt
      781 ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca
      841 tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct
      901 gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg
      961 cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt
     1021 tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga
     1081 gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg
     1141 ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg
     1201 gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta
     1261 tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca cccgaggggg
     1321 atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc
     1381 tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta
     1441 tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg
     1501 gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg
     1561 accgcctgaa gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat
     1621 ccatcttgct ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg
     1681 acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa
     1741 aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag
     1801 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca
     1861 gagagatcct cataaaggcc aagaagggcg gaaagatcgc cgtgtaa
//
