LOCUS       sequence-207866-        1878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Monitor the expression of Luciferase driven by a mutated NRAS 
            5'-UTR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Kumari S, Bugaut A, Huppert JL, Balasubramanian S
  TITLE     An RNA G-quadruplex in the 5' UTR of the NRAS proto-oncogene 
            modulates translation.
  JOURNAL   Nat Chem Biol. 2007 Apr;3(4):218-21. doi: 10.1038/nchembio864. Epub 
            2007 Feb 25.
  PUBMED    17322877
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio864"; journalName: "Nat Chem Biol"; date: 
            "2007-04"; volume: "3"; issue: "4"; pages: "218-21"
COMMENT     Sequence Label: pSKC12 - DelQ
FEATURES             Location/Qualifiers
     source          1..1878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             226..1878
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(292..310)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gggtgcggcc tgccgcatga ctcgtggttc ggaggcccac gtggccgggg cggggactca
       61 ggcgcctggg gcgccgactg attacgtagc gggcggggcc ggaagtgccg ctccttggtg
      121 ggggctgttc atggcggttc cggggtctcc aacatttttc ccggctgtgg tcctaaatct
      181 gtccaaagca gaggcagtgg agcttgaggt tcttgctggt gtgaaatgga agacgccaaa
      241 aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa
      301 ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca
      361 catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa
      421 gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct
      481 cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg
      541 aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg
      601 gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc
      661 atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac
      721 acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt gccagagtcc
      781 ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct
      841 aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct
      901 atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac
      961 ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg
     1021 tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat tcaaagtgcg
     1081 ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat
     1141 ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa
     1201 gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact
     1261 acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt
     1321 gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat
     1381 caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg
     1441 gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga catagcttac
     1501 tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa
     1561 ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc
     1621 gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt
     1681 gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa
     1741 gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt
     1801 cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc
     1861 ggaaagatcg ccgtgtaa
//
