LOCUS       sequence-207885-        9100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express yeast Fbp1-3xflag and reference flag-DHFR-ha protein, both 
            are contolled by tetracycline mediated TDH promter, test protein 
            expression and stability. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9100)
  AUTHORS   Chen SJ, Wu X, Wadas B, Oh JH, Varshavsky A
  TITLE     An N-end rule pathway that recognizes proline and destroys 
            gluconeogenic enzymes.
  JOURNAL   Science. 2017 Jan 27;355(6323). pii: 355/6323/eaal3655. doi: 
            10.1126/science.aal3655.
  PUBMED    28126757
REFERENCE   2  (bases 1 to 9100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-01-27"; volume: "355(6323). pii: 355/6323/eaal3655. 
            doi"; pages: " 10.1126/science.aal3655"
COMMENT     Sequence Label: pCSJ95
FEATURES             Location/Qualifiers
     source          1..9100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        317..504
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             505..1167
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     rep_origin      complement(1427..1882)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1564..1585)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1776..1795
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2008..2030
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2022..2039
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2023..2039
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2046..2065
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2046..2064
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2095..2761
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     CDS             4086..4151
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4202..4391
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        4420..5086
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     CDS             5364..5387
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5397..5957
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     CDS             5964..5990
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      6000..6247
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6282..6298)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6283..6299)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(6327..6345)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6366..6382)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6366..6382)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6379..6401)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6390..6406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6414..6420,6421..6438,6439..6444))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6459..6480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6597..6614)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6768..7356)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6848..6867)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7527..8318,8319..8387))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8150..8169
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8388..8492)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    8522..9025
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9073..9091
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
      361 tttttttttt ccacctagcg gatgactctt tttttttctt agcgattggc attatcacat
      421 aatgaattat acattatata aagtaatgtg atttcttcga agaatatact aaaaaatgag
      481 caggcaagat aaacgaaggc aaagatgaca gagcagaaag ccctagtaaa gcgtattaca
      541 aatgaaacca agattcagat tgcgatctct ttaaagggtg gtcccctagc gatagagcac
      601 tcgatcttcc cagaaaaaga ggcagaagca gtagcagaac aggccacaca atcgcaagtg
      661 attaacgtcc acacaggtat agggtttctg gaccatatga tacatgctct ggccaagcat
      721 tccggctggt cgctaatcgt tgagtgcatt ggtgacttac acatagacga ccatcacacc
      781 actgaagact gcgggattgc tctcggtcaa gcttttaaag aggccctagg ggccgtgcgt
      841 ggagtaaaaa ggtttggatc aggatttgcg cctttggatg aggcactttc cagagcggtg
      901 gtagatcttt cgaacaggcc gtacgcagtt gtcgaacttg gtttgcaaag ggagaaagta
      961 ggagatctct cttgcgagat gatcccgcat tttcttgaaa gctttgcaga ggctagcaga
     1021 attaccctcc acgttgattg tctgcgaggc aagaatgatc atcaccgtag tgagagtgcg
     1081 ttcaaggctc ttgcggttgc cataagagaa gccacctcgc ccaatggtac caacgatgtt
     1141 ccctccacca aaggtgttct tatgtagtga caccgattat ttaaagctgc agcatacgat
     1201 atatatacat gtgtatatat gtatacctat gaatgtcagt aagtatgtat acgaacagta
     1261 tgatactgaa gatgacaagg taatgcatca ttctatacgt gtcattctga acgaggcgcg
     1321 ctttcctttt ttctttttgc tttttctttt tttttctctt gaactcgacg gatctatgcg
     1381 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     1441 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     1501 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     1561 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     1621 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     1681 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     1741 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     1801 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     1861 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc
     1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     2041 aattgtaata cgactcacta tagggcgaat tggagctcca ccgcggcgac tttatcatta
     2101 tcaatactgc catttcaaag aatacgtaaa taattaatag tagtgatttt cctaacttta
     2161 tttagtcaaa aaattagcct tttaattctg ctgtaacccg tacatgccca aaataggggg
     2221 cgggttacac agaatatata acatcgtagg tgtctgggtg aacagtttat tcctggcatc
     2281 cactaaatat aatggagccc gctttttaag ctggcatcca gaaaaaaaaa gaatcccagc
     2341 accaaaatat tgttttcttc accaaccatc agttcatagg tccattctct tagcgcaact
     2401 acagagaaca ggggcacaaa caggcaaaaa acgggcacaa cctcaatgga gtgatgcaac
     2461 ctgcctggag taaatgatga cacaaggcaa ttgacccacg catgtatcta tctcattttc
     2521 ttacaccttc tattaccttc tgctctctct gatttggaaa aagctgaaaa aaaaggttga
     2581 aaccagttcc ctgaaattat tcccctactt gactaataag tatataaaga cggtaggtat
     2641 tgattgtaat tctgtaaatc tatttcttaa acttcttaaa ttctactttt atagttagtc
     2701 ttttttttag ttttaaaaca ccaagaactt agtttcgaat aaacacacat aaacaaacaa
     2761 aatcgtacgg gcctaaaaca taccagatcg ccacccgcgc tttaatctgg agaggtgaag
     2821 aatacgacca cctaggcctt aagaaccggt aaaacatacc agatcgccac ccgcgcttta
     2881 atctggagag gtgaagaata cgaccaccta ccggtcaaca acaacaacaa caacaacaac
     2941 aactcgaggc ctaaaacata ccagatcgcc acccgcgctt taatctggag aggtgaagaa
     3001 tacgaccacc taggcctcga gaacataggc gcgccatgcc aactctagta aatggaccaa
     3061 gaagagactc taccgaaggg tttgataccg atatcatcac tcttcctaga ttcataatcg
     3121 agcaccagaa gcaatttaag aacgctactg gtgatttcac attagtactg aatgccttgc
     3181 aattcgcgtt caaatttgta tctcacacca tcagacgtgc tgaattggtt aacttggttg
     3241 ggttagcagg cgcttccaac ttcactggtg accagcaaaa gaagttggac gttctaggtg
     3301 atgaaatatt tatcaatgcc atgagggcta gtgggatcat caaggtcctt gtatctgaag
     3361 aacaggaaga cttgatcgtt tttcccacaa acacgggctc atacgcagtg tgttgtgatc
     3421 ctattgatgg ctcctcaaat ttggacgccg gtgtctccgt tggaactatc gcgtctatat
     3481 tcagactgct accagactca tcaggtacta taaacgacgt actgagatgt ggtaaagaaa
     3541 tggtagccgc ttgctatgcc atgtacggat cctctacgca tctagtattg acattgggtg
     3601 atggagttga tgggtttacc ttagacacaa acttgggcga attcatcttg actcatccta
     3661 acttaagaat tccgcctcaa aaggccatct actcaattaa tgaaggtaac accctctact
     3721 ggaacgagac tataagaaca tttattgaga aagtcaaaca accccaagca gacaacaaca
     3781 acaagccttt ctcggctagg tatgttggat ccatggttgc tgatgttcac aggacgtttc
     3841 tttacggtgg ccttttcgca tacccttgcg acaagaagag ccccaacgga aaactgaggt
     3901 tgctttatga ggccttccca atggctttct taatggaaca agcaggggga aaagcggtca
     3961 acgatcgcgg agagagaatc ttggatttgg tgccaagtca tatccatgac aaatcttcta
     4021 tttggttggg ttcttcaggt gaaattgaca aatttttaga ccatattggc aagtcacaga
     4081 gatccgacta taaagaccat gacggtgatt ataaagatca tgatatcgat tacaaggatg
     4141 acgatgacaa gtaagcggcc gcattagtta tgtcacgctt acattcacgc cctcccccca
     4201 catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt
     4261 tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc
     4321 tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg
     4381 gacgctcgaa ggctttaatt tgcggccggc ccgactttat cattatcaat actgccattt
     4441 caaagaatac gtaaataatt aatagtagtg attttcctaa ctttatttag tcaaaaaatt
     4501 agccttttaa ttctgctgta acccgtacat gcccaaaata gggggcgggt tacacagaat
     4561 atataacatc gtaggtgtct gggtgaacag tttattcctg gcatccacta aatataatgg
     4621 agcccgcttt ttaagctggc atccagaaaa aaaaagaatc ccagcaccaa aatattgttt
     4681 tcttcaccaa ccatcagttc ataggtccat tctcttagcg caactacaga gaacaggggc
     4741 acaaacaggc aaaaaacggg cacaacctca atggagtgat gcaacctgcc tggagtaaat
     4801 gatgacacaa ggcaattgac ccacgcatgt atctatctca ttttcttaca ccttctatta
     4861 ccttctgctc tctctgattt ggaaaaagct gaaaaaaaag gttgaaacca gttccctgaa
     4921 attattcccc tacttgacta ataagtatat aaagacggta ggtattgatt gtaattctgt
     4981 aaatctattt cttaaacttc ttaaattcta cttttatagt tagtcttttt tttagtttta
     5041 aaacaccaag aacttagttt cgaataaaca cacataaaca aacaaaatcg tacgggccta
     5101 aaacatacca gatcgccacc cgcgctttaa tctggagagg tgaagaatac gaccacctag
     5161 gccttaagaa ccggtaaaac ataccagatc gccacccgcg ctttaatctg gagaggtgaa
     5221 gaatacgacc acctaccggt caacaacaac aacaacaaca acaacaactc gaggcctaaa
     5281 acataccaga tcgccacccg cgctttaatc tggagaggtg aagaatacga ccacctaggc
     5341 ctcgagaaca tatcgaattc atggactaca aggacgacga tgacaagggt gggtccatgg
     5401 ttcgaccatt gaactgcatc gtcgccgtgt cccaaaatat ggggattggc aagaacggag
     5461 acctaccctg gcctccgctc aggaacgagt tcaagtactt ccaaagaatg accacaacct
     5521 cttcagtgga aggtaaacag aatctggtga ttatgggtag gaaaacctgg ttctccattc
     5581 ctgagaagaa tcgaccttta aaggacagaa ttaatatagt tctcagtaga gaactcaaag
     5641 aaccaccacg aggcgctcat tttcttgcca aaagtttgga tgatgcctta agacttattg
     5701 aacaaccgga attggcaagt aaagtagaca tggtttggat agtcggaggc agttctgttt
     5761 accaggaagc catgaatcaa ccaggccacc tcagactctt tgtgacaagg atcatgcagg
     5821 aatttgaaag tgacacgttt ttcccagaaa ttgatttggg gaaatataaa cttctcccag
     5881 aatacccagg cgtcctctct gaggtccagg aggaaaaagg catcaagtat aagtttgaag
     5941 tctacgagaa gaaagacggt acctacccat acgatgtccc agattacgcc taactcgagt
     6001 catgtaatta gttatgtcac gcttacattc acgccctccc cccacatccg ctctaaccga
     6061 aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt
     6121 agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta
     6181 cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt
     6241 aatttgcggc ccgggctgca ggaattcgat atcaagctta tcgataccgt cgacctcgag
     6301 ggggggcccg gtaccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa
     6361 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6421 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6481 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6541 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6601 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6661 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6721 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6781 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6841 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6901 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6961 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7021 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7081 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7141 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7201 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7261 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7321 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7381 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7441 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7501 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7561 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7621 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7681 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7741 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7801 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7861 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7921 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7981 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8041 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8101 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8161 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8221 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8281 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8341 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8401 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8461 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8521 ggatcgcttg cctgtaactt acacgcgcct cgtatctttt aatgatggaa taatttggga
     8581 atttactctg tgtttattta tttttatgtt ttgtatttgg attttagaaa gtaaataaag
     8641 aaggtagaag agttacggaa tgaagaaaaa aaaataaaca aaggtttaaa aaatttcaac
     8701 aaaaagcgta ctttacatat atatttatta gacaagaaaa gcagattaaa tagatataca
     8761 ttcgattaac gataagtaaa atgtaaaatc acaggatttt cgtgtgtggt cttctacaca
     8821 gacaagatga aacaattcgg cattaatacc tgagagcagg aagagcaaga taaaaggtag
     8881 tatttgttgg cgatccccct agagtctttt acatcttcgg aaaacaaaaa ctattttttc
     8941 tttaatttct ttttttactt tctattttta atttatatat ttatattaaa aaatttaaat
     9001 tataattatt tttatagcac gtgatgaaaa ggaccctaag aaaccattat tatcatgaca
     9061 ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
