LOCUS       sequence-207894-        2447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Huang A, Nguyen PQ, Stark JC, Takahashi MK, Donghia N, Ferrante T, 
            Dy AJ, Hsu KJ, Dubner RS, Pardee K, Jewett MC, Collins JJ
  TITLE     BioBits Explorer: A modular synthetic biology education kit.
  JOURNAL   Sci Adv. 2018 Aug 1;4(8):eaat5105. doi: 10.1126/sciadv.aat5105. 
            eCollection 2018 Aug.
  PUBMED    30083608
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-08-1"; volume: "4(8):eaat5105. doi"; pages: " 
            10.1126/sciadv.aat5105. eCollection 2018 Aug"
COMMENT     Sequence Label: pJL1 eforRed
FEATURES             Location/Qualifiers
     source          1..2447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     617..636
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        617..635
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             667..689
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1432..1450)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1446..1493
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1511..2326)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2234..2253
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2318..2337
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga
      661 aataattttg tttaacttta agaaggagat atacatatga gcgtgattaa acaggtgatg
      721 aaaaccaaac tgcatctgga aggcaccgtt aatggtcatg atttcaccat tgaaggtaaa
      781 ggtgaaggca aaccgtatga aggtctgcag catatgaaaa tgaccgttac caaaggtgca
      841 ccgctgccgt ttagcgttca tattctgacc ccgagccata tgtatggtag caaaccgttt
      901 aacaaatatc cggcagatat cccggattat cacaaacaga gctttccgga aggtatgagc
      961 tgggaacgta gcatgatttt tgaagatggt ggtgtttgta ccgcaagcaa tcatagcagc
     1021 attaatctgc aagaaaactg cttcatctac gacgtgaaat tccatggtgt taatctgcct
     1081 ccggatggtc cggttatgca gaaaaccatt gcaggttggg aaccgagcgt tgaaaccctg
     1141 tatgttcgtg atggtatgct gaaaagcgat accgccatgg tttttaaact gaaaggtggt
     1201 ggtcatcatc gtgtggattt caaaaccacc tacaaagcaa aaaaaccggt taaactgccg
     1261 gaattccatt ttgttgaaca tcgtctggaa ctgaccaaac acgataaaga ttttaccacc
     1321 tgggatcagc aagaagcagc agaaggtcat tttagtccgc tgccgaaagc actgccgtaa
     1381 gtcgaccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     1441 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     1501 ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag
     1561 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga
     1621 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat
     1681 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat
     1741 gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc cagacttgtt
     1801 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca
     1861 ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa
     1921 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg
     1981 aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta
     2041 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg
     2101 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat
     2161 gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg
     2221 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat
     2281 ttaatcgcgg cttcgagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat
     2341 tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa
     2401 tgtaacatca gagattttga gacacaacgt gagatcaaag gatcttc
//
