LOCUS       sequence-207900-        5951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Taylor MJ, Perrais D, Merrifield CJ
  TITLE     A high precision survey of the molecular dynamics of Mammalian 
            clathrin-mediated endocytosis.
  JOURNAL   PLoS Biol. 2011 Mar . 9(3):e1000604.
  PUBMED    21445324
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2011 Mar . 9(3):e1000604."
COMMENT     Sequence Label: Arp3-pmCherryC1
FEATURES             Location/Qualifiers
     source          1..5951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             540..1247
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(685..703)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     953..972
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2679..2800
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2716..2735)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2770..2789
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2807..3262)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2944..2965)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3156..3175
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3289..3393
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3395..3752
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3395..3586
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3418..3438)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3603..3738
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3787..4581
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3787..4581
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3841..3860)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4451..4470
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4769..4788)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4813..4860
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5189..5777
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5678..5697
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcacca
      541 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
      601 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
      661 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
      721 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
      781 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
      841 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
      901 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
      961 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     1021 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     1081 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     1141 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     1201 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtcc ggactcagat
     1261 ctcgagctca agcttcgtct ggaggttctg gtatggcggg acggctgccg gcctgtgtgg
     1321 tggactgtgg cacggggtat acaaaactag gatatgctgg aaatacagaa ccacagttta
     1381 tcatcccttc ctgtattgct attaaggagt cagcaaaagt gggtgatcaa gctcaaagga
     1441 gggtgatgaa aggtgttgat gacctagact tcttcattgg tgatgaagca atagaaaaac
     1501 ctacatatgc aacaaagtgg ccaatccgcc atggtatagt tgaagattgg gacttaatgg
     1561 aaaggtttat ggagcaagtg atctttaaat atttaagggc agaacctgaa gaccattatt
     1621 ttcttttgac tgaacctcca ttgaatactc cagaaaacag ggaatatact gctgaaataa
     1681 tgtttgagtc cttcaatgtt ccaggcttgt acattgctgt gcaggctgtt cttgccttag
     1741 ctgcatcttg gacctcaaga caagtaggag aacggacgtt gaccggtacg gtaatagaca
     1801 gtggagatgg tgtcactcat gtcattcctg tggctgaagg gtatgtgatt ggcagctgta
     1861 ttaaacacat tccaatcgca ggacgagata taacatattt tattcagcaa ctgctgagag
     1921 accgagaagt aggaatccct ccagaacaat ccttggaaac tgctaaggca gtaaaggagc
     1981 gctatagtta tgtctgccca gatttagtaa aagaatttaa caagtatgat acagatgggt
     2041 caaaatggat taaacagtat actggaatca atgctatctc aaagaaagag ttttctatcg
     2101 atgttggtta tgagagattt ttgggacctg aaatcttttt tcatccagag tttgctaatc
     2161 cagactttac acaacctatc tcagaagttg tagatgaagt aattcagaat tgtcctattg
     2221 atgtcagacg tcctctctac aagaatattg tcctctctgg aggttcaacc atgttcaggg
     2281 actttggacg tcgcttgcaa agagatttga aaagaactgt agatgcccgg ctgaaattaa
     2341 gtgaggaatt gagtggtggt agattgaagc caaaacctat tgatgtacaa gtcattacac
     2401 accacatgca gcgatatgca gtttggtttg gaggatcaat gctggcttcc acgcctgagt
     2461 tctaccaagt atgccacacc aaaaaggatt atgaagaaat tggacctagc atttgtcgtc
     2521 acaatccagt gtttggagtc atgtcgtaag gatccaccgg atctagataa ctgatcataa
     2581 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     2641 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     2701 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     2761 attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt
     2821 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     2881 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     2941 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     3001 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     3061 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     3121 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     3181 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     3241 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     3301 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3361 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga
     3421 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3481 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     3541 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     3601 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     3661 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     3721 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
     3781 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     3841 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     3901 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     3961 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     4021 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     4081 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     4141 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     4201 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     4261 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
     4321 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     4381 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     4441 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     4501 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     4561 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     4621 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     4681 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     4741 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     4801 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
     4861 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     4921 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     4981 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
     5041 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5101 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5161 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5221 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5281 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5341 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5401 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5461 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5521 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5581 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5641 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5701 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5761 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5821 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5881 ccgccatgca ttagttatta atagtaatca attacggggt cattagttca tagcccatat
     5941 atggagttcc g
//
