LOCUS       sequence-207903-        8791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both 
            are contolled by tetracycline mediated TDH promter, test protein 
            expression and stability..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8791)
  AUTHORS   Oh JH, Chen SJ, Varshavsky A
  TITLE     A reference-based protein degradation assay without global 
            translation inhibitors.
  JOURNAL   J Biol Chem. 2017 Dec 29;292(52):21457-21465. doi: 
            10.1074/jbc.M117.814236. Epub 2017 Nov 9.
  PUBMED    29122887
REFERENCE   2  (bases 1 to 8791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M117.814236"; journalName: "J Biol Chem"; date: 
            "2017-12-29- 29"; volume: "292"; issue: "52"; pages: "21457-21465"
COMMENT     Sequence Label: pJO629
FEATURES             Location/Qualifiers
     source          1..8791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        317..504
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             505..1167
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     rep_origin      complement(1427..1882)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1564..1585)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1776..1795
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2008..2030
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2022..2039
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2023..2039
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2046..2065
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2046..2064
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2095..2761
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     CDS             3777..3842
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3893..4082
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        4111..4777
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     CDS             5055..5078
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5088..5648
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     CDS             5655..5681
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      5691..5938
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5973..5989)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5974..5990)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(6018..6036)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6057..6073)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6057..6073)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6070..6092)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6081..6097
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6105..6111,6112..6129,6130..6135))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6150..6171
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6288..6305)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6459..7047)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6539..6558)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7218..8009,8010..8078))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7841..7860
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8079..8183)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    8213..8716
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     8764..8782
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
      361 tttttttttt ccacctagcg gatgactctt tttttttctt agcgattggc attatcacat
      421 aatgaattat acattatata aagtaatgtg atttcttcga agaatatact aaaaaatgag
      481 caggcaagat aaacgaaggc aaagatgaca gagcagaaag ccctagtaaa gcgtattaca
      541 aatgaaacca agattcagat tgcgatctct ttaaagggtg gtcccctagc gatagagcac
      601 tcgatcttcc cagaaaaaga ggcagaagca gtagcagaac aggccacaca atcgcaagtg
      661 attaacgtcc acacaggtat agggtttctg gaccatatga tacatgctct ggccaagcat
      721 tccggctggt cgctaatcgt tgagtgcatt ggtgacttac acatagacga ccatcacacc
      781 actgaagact gcgggattgc tctcggtcaa gcttttaaag aggccctagg ggccgtgcgt
      841 ggagtaaaaa ggtttggatc aggatttgcg cctttggatg aggcactttc cagagcggtg
      901 gtagatcttt cgaacaggcc gtacgcagtt gtcgaacttg gtttgcaaag ggagaaagta
      961 ggagatctct cttgcgagat gatcccgcat tttcttgaaa gctttgcaga ggctagcaga
     1021 attaccctcc acgttgattg tctgcgaggc aagaatgatc atcaccgtag tgagagtgcg
     1081 ttcaaggctc ttgcggttgc cataagagaa gccacctcgc ccaatggtac caacgatgtt
     1141 ccctccacca aaggtgttct tatgtagtga caccgattat ttaaagctgc agcatacgat
     1201 atatatacat gtgtatatat gtatacctat gaatgtcagt aagtatgtat acgaacagta
     1261 tgatactgaa gatgacaagg taatgcatca ttctatacgt gtcattctga acgaggcgcg
     1321 ctttcctttt ttctttttgc tttttctttt tttttctctt gaactcgacg gatctatgcg
     1381 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     1441 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     1501 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     1561 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     1621 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     1681 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     1741 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     1801 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     1861 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc
     1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     2041 aattgtaata cgactcacta tagggcgaat tggagctcca ccgcggcgac tttatcatta
     2101 tcaatactgc catttcaaag aatacgtaaa taattaatag tagtgatttt cctaacttta
     2161 tttagtcaaa aaattagcct tttaattctg ctgtaacccg tacatgccca aaataggggg
     2221 cgggttacac agaatatata acatcgtagg tgtctgggtg aacagtttat tcctggcatc
     2281 cactaaatat aatggagccc gctttttaag ctggcatcca gaaaaaaaaa gaatcccagc
     2341 accaaaatat tgttttcttc accaaccatc agttcatagg tccattctct tagcgcaact
     2401 acagagaaca ggggcacaaa caggcaaaaa acgggcacaa cctcaatgga gtgatgcaac
     2461 ctgcctggag taaatgatga cacaaggcaa ttgacccacg catgtatcta tctcattttc
     2521 ttacaccttc tattaccttc tgctctctct gatttggaaa aagctgaaaa aaaaggttga
     2581 aaccagttcc ctgaaattat tcccctactt gactaataag tatataaaga cggtaggtat
     2641 tgattgtaat tctgtaaatc tatttcttaa acttcttaaa ttctactttt atagttagtc
     2701 ttttttttag ttttaaaaca ccaagaactt agtttcgaat aaacacacat aaacaaacaa
     2761 aatcgtacgg gcctaaaaca taccagatcg ccacccgcgc tttaatctgg agaggtgaag
     2821 aatacgacca cctaggcctt aagaaccggt aaaacatacc agatcgccac ccgcgcttta
     2881 atctggagag gtgaagaata cgaccaccta ccggtcaaca acaacaacaa caacaacaac
     2941 aactcgaggc ctaaaacata ccagatcgcc acccgcgctt taatctggag aggtgaagaa
     3001 tacgaccacc taggcctcga gaacataggc gcgccatggc tctacctctg ataaaaccta
     3061 aggagtctga ggaaagccac ctcgctcttt tgtccaagat ccacgtgtcc aaaaattgga
     3121 aactgcctcc aaggttgccc catagagcag ctcagcggag gaaaagagtt catcgacttc
     3181 acgaagatta cgaaactgaa gaaaatgatg aggagctaca gaagaaaaaa cggcagaata
     3241 gagacgccca aagggcctac agagaacgca aaaacaacaa gttgcaagtg ctggaggaaa
     3301 ccatagagtc actgagtaag gtcgttaaaa actacgaaac gaaactaaac cgcctccaaa
     3361 atgaacttca agcgaaagaa tcggaaaatc atgcccttaa acagaaattg gagaccctta
     3421 ctctgaaaca agcctctgtg cctgctcagg accctatcct gcagaatttg atcgagaatt
     3481 tcaaaccaat gaaggcgata cctattaagt acaataccgc tatcaagcgc caccagcact
     3541 cgacagaact gcctagctct gtcaaatgcg gcttttgtaa cgacaacact acttgcgttt
     3601 gtaaagaact ggaaacagat caccgtaaaa gtgacgacgg tgtggccaca gaacagaagg
     3661 atatgtcgat gccacatgca gagtgcaata ataaagataa ccccaatggg ctctgtagta
     3721 attgtactaa tatcgacaaa tcatgtatcg acatccggtc catcatccac ggatccgact
     3781 ataaagacca tgacggtgat tataaagatc atgatatcga ttacaaggat gacgatgaca
     3841 agtaagcggc cgcattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc
     3901 taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag
     3961 ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac
     4021 gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga
     4081 aggctttaat ttgcggccgg cccgacttta tcattatcaa tactgccatt tcaaagaata
     4141 cgtaaataat taatagtagt gattttccta actttattta gtcaaaaaat tagcctttta
     4201 attctgctgt aacccgtaca tgcccaaaat agggggcggg ttacacagaa tatataacat
     4261 cgtaggtgtc tgggtgaaca gtttattcct ggcatccact aaatataatg gagcccgctt
     4321 tttaagctgg catccagaaa aaaaaagaat cccagcacca aaatattgtt ttcttcacca
     4381 accatcagtt cataggtcca ttctcttagc gcaactacag agaacagggg cacaaacagg
     4441 caaaaaacgg gcacaacctc aatggagtga tgcaacctgc ctggagtaaa tgatgacaca
     4501 aggcaattga cccacgcatg tatctatctc attttcttac accttctatt accttctgct
     4561 ctctctgatt tggaaaaagc tgaaaaaaaa ggttgaaacc agttccctga aattattccc
     4621 ctacttgact aataagtata taaagacggt aggtattgat tgtaattctg taaatctatt
     4681 tcttaaactt cttaaattct acttttatag ttagtctttt ttttagtttt aaaacaccaa
     4741 gaacttagtt tcgaataaac acacataaac aaacaaaatc gtacgggcct aaaacatacc
     4801 agatcgccac ccgcgcttta atctggagag gtgaagaata cgaccaccta ggccttaaga
     4861 accggtaaaa cataccagat cgccacccgc gctttaatct ggagaggtga agaatacgac
     4921 cacctaccgg tcaacaacaa caacaacaac aacaacaact cgaggcctaa aacataccag
     4981 atcgccaccc gcgctttaat ctggagaggt gaagaatacg accacctagg cctcgagaac
     5041 atatcgaatt catggactac aaggacgacg atgacaaggg tgggtccatg gttcgaccat
     5101 tgaactgcat cgtcgccgtg tcccaaaata tggggattgg caagaacgga gacctaccct
     5161 ggcctccgct caggaacgag ttcaagtact tccaaagaat gaccacaacc tcttcagtgg
     5221 aaggtaaaca gaatctggtg attatgggta ggaaaacctg gttctccatt cctgagaaga
     5281 atcgaccttt aaaggacaga attaatatag ttctcagtag agaactcaaa gaaccaccac
     5341 gaggcgctca ttttcttgcc aaaagtttgg atgatgcctt aagacttatt gaacaaccgg
     5401 aattggcaag taaagtagac atggtttgga tagtcggagg cagttctgtt taccaggaag
     5461 ccatgaatca accaggccac ctcagactct ttgtgacaag gatcatgcag gaatttgaaa
     5521 gtgacacgtt tttcccagaa attgatttgg ggaaatataa acttctccca gaatacccag
     5581 gcgtcctctc tgaggtccag gaggaaaaag gcatcaagta taagtttgaa gtctacgaga
     5641 agaaagacgg tacctaccca tacgatgtcc cagattacgc ctaactcgag tcatgtaatt
     5701 agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg
     5761 agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag
     5821 aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa
     5881 cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcgg
     5941 cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc
     6001 ggtaccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca
     6061 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6121 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6181 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6241 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6301 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6361 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6421 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6481 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6541 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6601 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6661 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6721 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6781 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6841 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6901 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6961 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7021 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7081 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7141 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7201 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7261 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7321 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7381 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7441 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7501 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7561 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7621 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7681 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7741 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7801 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7861 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7921 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7981 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8041 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8101 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8161 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cggatcgctt
     8221 gcctgtaact tacacgcgcc tcgtatcttt taatgatgga ataatttggg aatttactct
     8281 gtgtttattt atttttatgt tttgtatttg gattttagaa agtaaataaa gaaggtagaa
     8341 gagttacgga atgaagaaaa aaaaataaac aaaggtttaa aaaatttcaa caaaaagcgt
     8401 actttacata tatatttatt agacaagaaa agcagattaa atagatatac attcgattaa
     8461 cgataagtaa aatgtaaaat cacaggattt tcgtgtgtgg tcttctacac agacaagatg
     8521 aaacaattcg gcattaatac ctgagagcag gaagagcaag ataaaaggta gtatttgttg
     8581 gcgatccccc tagagtcttt tacatcttcg gaaaacaaaa actatttttt ctttaatttc
     8641 tttttttact ttctattttt aatttatata tttatattaa aaaatttaaa ttataattat
     8701 ttttatagca cgtgatgaaa aggaccctaa gaaaccatta ttatcatgac attaacctat
     8761 aaaaataggc gtatcacgag gccctttcgt c
//
