LOCUS       sequence-207905-        9187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express yeast MDH2-3xflag and reference flag-DHFR-ha protein, both 
            are contolled by tetracycline mediated TDH promter, test protein 
            expression and stability..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Chen SJ, Wu X, Wadas B, Oh JH, Varshavsky A
  TITLE     An N-end rule pathway that recognizes proline and destroys 
            gluconeogenic enzymes.
  JOURNAL   Science. 2017 Jan 27;355(6323). pii: 355/6323/eaal3655. doi: 
            10.1126/science.aal3655.
  PUBMED    28126757
REFERENCE   2  (bases 1 to 9187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-01-27"; volume: "355(6323). pii: 355/6323/eaal3655. 
            doi"; pages: " 10.1126/science.aal3655"
COMMENT     Sequence Label: pCSJ125
FEATURES             Location/Qualifiers
     source          1..9187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        317..504
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             505..1167
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     rep_origin      complement(1427..1882)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1564..1585)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1776..1795
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2008..2030
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2022..2039
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2023..2039
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2046..2065
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2046..2064
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2095..2761
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     CDS             4173..4238
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4289..4478
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        4507..5173
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     CDS             5451..5474
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5484..6044
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     CDS             6051..6077
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      6087..6334
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6369..6385)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6370..6386)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(6414..6432)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6453..6469)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6453..6469)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6466..6488)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6477..6493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6501..6507,6508..6525,6526..6531))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6546..6567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6684..6701)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6855..7443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6935..6954)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7614..8405,8406..8474))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8237..8256
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8475..8579)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    8609..9112
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9160..9178
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
      361 tttttttttt ccacctagcg gatgactctt tttttttctt agcgattggc attatcacat
      421 aatgaattat acattatata aagtaatgtg atttcttcga agaatatact aaaaaatgag
      481 caggcaagat aaacgaaggc aaagatgaca gagcagaaag ccctagtaaa gcgtattaca
      541 aatgaaacca agattcagat tgcgatctct ttaaagggtg gtcccctagc gatagagcac
      601 tcgatcttcc cagaaaaaga ggcagaagca gtagcagaac aggccacaca atcgcaagtg
      661 attaacgtcc acacaggtat agggtttctg gaccatatga tacatgctct ggccaagcat
      721 tccggctggt cgctaatcgt tgagtgcatt ggtgacttac acatagacga ccatcacacc
      781 actgaagact gcgggattgc tctcggtcaa gcttttaaag aggccctagg ggccgtgcgt
      841 ggagtaaaaa ggtttggatc aggatttgcg cctttggatg aggcactttc cagagcggtg
      901 gtagatcttt cgaacaggcc gtacgcagtt gtcgaacttg gtttgcaaag ggagaaagta
      961 ggagatctct cttgcgagat gatcccgcat tttcttgaaa gctttgcaga ggctagcaga
     1021 attaccctcc acgttgattg tctgcgaggc aagaatgatc atcaccgtag tgagagtgcg
     1081 ttcaaggctc ttgcggttgc cataagagaa gccacctcgc ccaatggtac caacgatgtt
     1141 ccctccacca aaggtgttct tatgtagtga caccgattat ttaaagctgc agcatacgat
     1201 atatatacat gtgtatatat gtatacctat gaatgtcagt aagtatgtat acgaacagta
     1261 tgatactgaa gatgacaagg taatgcatca ttctatacgt gtcattctga acgaggcgcg
     1321 ctttcctttt ttctttttgc tttttctttt tttttctctt gaactcgacg gatctatgcg
     1381 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     1441 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     1501 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     1561 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     1621 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     1681 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     1741 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     1801 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     1861 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc
     1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     2041 aattgtaata cgactcacta tagggcgaat tggagctcca ccgcggcgac tttatcatta
     2101 tcaatactgc catttcaaag aatacgtaaa taattaatag tagtgatttt cctaacttta
     2161 tttagtcaaa aaattagcct tttaattctg ctgtaacccg tacatgccca aaataggggg
     2221 cgggttacac agaatatata acatcgtagg tgtctgggtg aacagtttat tcctggcatc
     2281 cactaaatat aatggagccc gctttttaag ctggcatcca gaaaaaaaaa gaatcccagc
     2341 accaaaatat tgttttcttc accaaccatc agttcatagg tccattctct tagcgcaact
     2401 acagagaaca ggggcacaaa caggcaaaaa acgggcacaa cctcaatgga gtgatgcaac
     2461 ctgcctggag taaatgatga cacaaggcaa ttgacccacg catgtatcta tctcattttc
     2521 ttacaccttc tattaccttc tgctctctct gatttggaaa aagctgaaaa aaaaggttga
     2581 aaccagttcc ctgaaattat tcccctactt gactaataag tatataaaga cggtaggtat
     2641 tgattgtaat tctgtaaatc tatttcttaa acttcttaaa ttctactttt atagttagtc
     2701 ttttttttag ttttaaaaca ccaagaactt agtttcgaat aaacacacat aaacaaacaa
     2761 aatcgtacgg gcctaaaaca taccagatcg ccacccgcgc tttaatctgg agaggtgaag
     2821 aatacgacca cctaggcctt aagaaccggt aaaacatacc agatcgccac ccgcgcttta
     2881 atctggagag gtgaagaata cgaccaccta ccggtcaaca acaacaacaa caacaacaac
     2941 aactcgaggc ctaaaacata ccagatcgcc acccgcgctt taatctggag aggtgaagaa
     3001 tacgaccacc taggcctcga gaacataggc gcgccatgcc tcactcagtt acaccatcca
     3061 tagaacaaga ttcgttaaaa attgccattt taggtgctgc cggtggtatc gggcagtcgt
     3121 tatcgctgct tttgaaagct cagttgcaat accagttaaa ggagagcaac cggagcgtta
     3181 cccacattca tctggctctt tacgatgtca accaagaagc catcaacggt gttaccgccg
     3241 acttgtctca tatagacacc cccatttccg tgtcgagcca ctctcctgca ggtggcattg
     3301 agaactgttt gcataacgct tctattgttg tcattcctgc aggtgttcca agaaaacctg
     3361 gcatgactcg tgatgactta tttaacgtga atgctggtat cattagccag ctcggtgatt
     3421 ctattgcaga atgttgtgat ctttccaagg tcttcgttct tgtcatttcc aaccctgtta
     3481 attctttagt cccagtgatg gtttctaaca ttcttaagaa ccatcctcag tctagaaatt
     3541 ccggcattga aagaaggatc atgggtgtca ccaagctcga cattgtcaga gcgtccactt
     3601 ttctacgtga gataaacatt gagtcagggc taactcctcg tgttaactcc atgcctgacg
     3661 tccctgtaat tggcgggcat tctggcgaga ctattattcc gttgttttca cagtcaaact
     3721 tcctatcgag attaaatgag gatcaattga aatatttaat acatagagtc caatacggtg
     3781 gtgatgaagt ggtcaaggcc aagaacggta aaggtagtgc taccttatcg atggcccatg
     3841 ccggttataa gtgtgttgtc caatttgttt ctttgttatt gggtaacatt gagcagatcc
     3901 atggaaccta ctatgtgcca ttaaaagatg cgaacaactt ccccattgct cctggggcag
     3961 atcaattatt gcctctggtg gacggtgcag actactttgc cataccatta actattacta
     4021 caaagggtgt ttcctatgtg gattatgaca tcgttaatag gatgaacgac atggaacgca
     4081 accaaatgtt gccaatttgc gtctcccagt taaagaaaaa tatcgataag ggcttggaat
     4141 tcgttgcatc gagatctgca tcatctggat ccgactataa agaccatgac ggtgattata
     4201 aagatcatga tatcgattac aaggatgacg atgacaagta agcggccgca ttagttatgt
     4261 cacgcttaca ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac
     4321 aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat
     4381 ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac
     4441 tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc ggccggcccg
     4501 actttatcat tatcaatact gccatttcaa agaatacgta aataattaat agtagtgatt
     4561 ttcctaactt tatttagtca aaaaattagc cttttaattc tgctgtaacc cgtacatgcc
     4621 caaaataggg ggcgggttac acagaatata taacatcgta ggtgtctggg tgaacagttt
     4681 attcctggca tccactaaat ataatggagc ccgcttttta agctggcatc cagaaaaaaa
     4741 aagaatccca gcaccaaaat attgttttct tcaccaacca tcagttcata ggtccattct
     4801 cttagcgcaa ctacagagaa caggggcaca aacaggcaaa aaacgggcac aacctcaatg
     4861 gagtgatgca acctgcctgg agtaaatgat gacacaaggc aattgaccca cgcatgtatc
     4921 tatctcattt tcttacacct tctattacct tctgctctct ctgatttgga aaaagctgaa
     4981 aaaaaaggtt gaaaccagtt ccctgaaatt attcccctac ttgactaata agtatataaa
     5041 gacggtaggt attgattgta attctgtaaa tctatttctt aaacttctta aattctactt
     5101 ttatagttag tctttttttt agttttaaaa caccaagaac ttagtttcga ataaacacac
     5161 ataaacaaac aaaatcgtac gggcctaaaa cataccagat cgccacccgc gctttaatct
     5221 ggagaggtga agaatacgac cacctaggcc ttaagaaccg gtaaaacata ccagatcgcc
     5281 acccgcgctt taatctggag aggtgaagaa tacgaccacc taccggtcaa caacaacaac
     5341 aacaacaaca acaactcgag gcctaaaaca taccagatcg ccacccgcgc tttaatctgg
     5401 agaggtgaag aatacgacca cctaggcctc gagaacatat cgaattcatg gactacaagg
     5461 acgacgatga caagggtggg tccatggttc gaccattgaa ctgcatcgtc gccgtgtccc
     5521 aaaatatggg gattggcaag aacggagacc taccctggcc tccgctcagg aacgagttca
     5581 agtacttcca aagaatgacc acaacctctt cagtggaagg taaacagaat ctggtgatta
     5641 tgggtaggaa aacctggttc tccattcctg agaagaatcg acctttaaag gacagaatta
     5701 atatagttct cagtagagaa ctcaaagaac caccacgagg cgctcatttt cttgccaaaa
     5761 gtttggatga tgccttaaga cttattgaac aaccggaatt ggcaagtaaa gtagacatgg
     5821 tttggatagt cggaggcagt tctgtttacc aggaagccat gaatcaacca ggccacctca
     5881 gactctttgt gacaaggatc atgcaggaat ttgaaagtga cacgtttttc ccagaaattg
     5941 atttggggaa atataaactt ctcccagaat acccaggcgt cctctctgag gtccaggagg
     6001 aaaaaggcat caagtataag tttgaagtct acgagaagaa agacggtacc tacccatacg
     6061 atgtcccaga ttacgcctaa ctcgagtcat gtaattagtt atgtcacgct tacattcacg
     6121 ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt
     6181 ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt
     6241 tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga
     6301 gaaggttttg ggacgctcga aggctttaat ttgcggcccg ggctgcagga attcgatatc
     6361 aagcttatcg ataccgtcga cctcgagggg gggcccggta ccagcttttg ttccctttag
     6421 tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6481 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6541 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6601 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6661 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6721 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6781 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6841 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6901 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6961 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7021 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7081 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7141 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7201 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7261 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7321 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7381 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7441 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7501 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7561 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7621 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7681 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7741 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7801 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7861 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7921 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7981 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8041 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8101 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8161 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8221 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8281 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8341 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8401 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8461 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8521 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8581 acatttcccc gaaaagtgcc acctgacgga tcgcttgcct gtaacttaca cgcgcctcgt
     8641 atcttttaat gatggaataa tttgggaatt tactctgtgt ttatttattt ttatgttttg
     8701 tatttggatt ttagaaagta aataaagaag gtagaagagt tacggaatga agaaaaaaaa
     8761 ataaacaaag gtttaaaaaa tttcaacaaa aagcgtactt tacatatata tttattagac
     8821 aagaaaagca gattaaatag atatacattc gattaacgat aagtaaaatg taaaatcaca
     8881 ggattttcgt gtgtggtctt ctacacagac aagatgaaac aattcggcat taatacctga
     8941 gagcaggaag agcaagataa aaggtagtat ttgttggcga tccccctaga gtcttttaca
     9001 tcttcggaaa acaaaaacta ttttttcttt aatttctttt tttactttct atttttaatt
     9061 tatatattta tattaaaaaa tttaaattat aattattttt atagcacgtg atgaaaagga
     9121 ccctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     9181 tttcgtc
//
