LOCUS       sequence-207910-        5414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used to drive expression specifically in zebrafish motor 
            neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: p5E--3mnx1
FEATURES             Location/Qualifiers
     source          1..5414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    3460..3583
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3655..3674)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3656..3674)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3679..3695)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3679..3695)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3797..3816)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3808..4617
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3875..3894)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4764..5352
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5253..5272
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgct gaggaaaacg ctttcgacta cagaaggact
      721 ttgtattatg caatgttttc aaaaaaatat cttcatattc agtgtgggag ataataagaa
      781 aggaaaaagc atgaagcact gaatcggtgt cctttaatga cttttatagt tgggaacaat
      841 tagccgttct ccacaaatta aattgataaa aagattacga gatccaaatg cgtttaaaag
      901 ctgtggtacg cacggcccat ttgtgcaatt atgatcgaaa atatttattg caataaaaga
      961 gacagtgcag gtcccgttaa attggctttt cataatgaac acatttaaca tggataatta
     1021 atatataaac tatttcgatt tgtcacgatg atatgtgttg cacttaattg tgaaagtaat
     1081 taccgagcga atgaacatct ttgaggagag tccctggttt caggccctcg tatcatcggg
     1141 tgcaataata attttactct ctcctgatgt gatgaaaaag agccataaat ctctcagatc
     1201 gctgcaggtc cacggcttca tatttcaccg ctgctttaat agaaatattc atgcgatccc
     1261 ttaatgaaat taaaactgag aagcggacaa agcggcccta ggctatgtgc aacgtgtaaa
     1321 gacacaagag catttgattg ggaaaatgtt gcatccttca aatatgagga tgttgttgca
     1381 ctgtaaacca cgtaaaacaa tttttttctt acagtccagt attggccaca cttgataatt
     1441 gttacatttg ttatcaatta cacgcgaggc tcattgaatg tcatttcaga attggtcagt
     1501 gcatgcgtca ttacggcacg cttttattct catcctttcg gccggtgact tgttgatttc
     1561 cctcgagcaa ataaacgcat gcgaccagtc tctcgagagt ggttagctga tgaattgagg
     1621 aaaactaatc cgctttattg gtagacaggt taagggcaac agagtgtcaa taattctctc
     1681 cctgacctct ttctccatta gcttaaagag gcttataaga ggaacagacc ccacaacatg
     1741 acaccgatcc tccacatcac catctcacat gtagcctacc taggcttgga aaatcatttc
     1801 gatttataaa gctcaagatc caacatttgc caaaaattca tgaaaacagg gcacaaatag
     1861 gacatcattt tctcacaaag tattatatag gcctaattag atagatagat agatagatag
     1921 atagatagat agatagatag atagatagat agatagatag atagatagat agatagatag
     1981 atagatagat agatagatag atagatagat agatagatag aaaaaccaag attttgcttg
     2041 tgattcatta tgcataataa tttgcctatg tttattaata aacatcgtgt taggattata
     2101 aatgtgttgt gatgaatgtg attaaatgtt tactgacagt tgttagccta tatttacaga
     2161 tgtaaaaaat ctacgtctaa ttagtatgtt cttcttaatc tataataagt aaaggcttat
     2221 taagcttgtc tttacaattt agaatttacg aatttatcct ccagagaaaa aaaaatgtaa
     2281 taacaccata tttaaatatc ctgttgagaa attatgaaat ccaattttca cattctaaga
     2341 ccaaggttaa atattgacag ttgacagttg ttttattttt taagcatgtt gtggtgggtg
     2401 agttttcaaa gatttacaca tttcaaacaa tttattaaaa caatatttgt tgcataatac
     2461 actgatttgg tcagttttac cactgacttt aactaattta tttaattaat tatatttata
     2521 ttgttcattg ttattaaatt ttgcctacct aatgctctct aacaaataag gaataacttg
     2581 aatatttcct ctgcgcaaaa tgttcatcgt aatgtaaaag atccgtttga catttaaaat
     2641 ggttctcttc tcataaagat agtaattctt ttaagaactg tcctctaaaa ggttctttgg
     2701 ggaaacaaaa aaatatacgt gtacaacatc aatgtgaaaa tcaaatagtt ttttttatta
     2761 tcgcaggctg ttcagataaa aaaatacaat tgaataaaaa agtatggaca ttaaaatggt
     2821 gtcatagcaa aatgacaaat gcgtgtatag actatggttt tttgttaatt aatttgatga
     2881 aataaaaaaa aattatacaa atgaaaaaaa tgtcatgcct ctacatctat gcgaataaaa
     2941 tgcatggctg tttttttttt ttgttcaaac aaactatttc gccaaaataa tattttcgag
     3001 ttaacgtgct ttatcaatct tcgtttatat taagattata ggtcggtgtt taaataatga
     3061 aatcttgata actttataaa tgtttcatgt ttcaaagttg catgttaata attgcccttt
     3121 agactctaca aatctggaat tattcataaa ttaagtagat gtaaaatgta tgtgtgcatg
     3181 ttgtgtttcc cacttaaaaa gctttcattt tatgggagaa catacttatg acaactcttg
     3241 aacttctaca agcacaccgt gaacactgga atcactgtcc aatcggaaat gcacaagaat
     3301 gacgtgaatg accaatccac tccctctcgg gcagcagttg acaagtggac ttctggcttg
     3361 cacaccttgg ccaagatcat aactttacga agaggagcgg tgacactcct gacagccttt
     3421 taaagttacg aacgacgcgt taatctgttt gtgagttggc aagtttgtac aaaaaagttg
     3481 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     3541 agactacata atactgtaaa acacaacata tgcagtcact atgaaccaac tacttagatg
     3601 gtattagtga cctgtagaat tcgagctcta gagctgcagg gcggccgcga tatcccctat
     3661 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     3721 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     3781 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
     3841 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     3901 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     3961 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     4021 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     4081 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     4141 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     4201 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     4261 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     4321 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     4381 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     4441 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     4501 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     4561 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     4621 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     4681 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     4741 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4801 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4861 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     4921 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4981 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5041 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5101 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5161 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5221 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5281 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5341 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5401 tttgctcaca tgtt
//
