LOCUS       sequence-207919-        2411 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses a FLAG-tagged human ZYG11A protein in mamalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Balachandran RS, Heighington CS, Starostina NG, Anderson JW, Owen 
            DL, Vasudevan S, Kipreos ET
  TITLE     The ubiquitin ligase CRL2ZYG11 targets cyclin B1 for degradation in 
            a conserved pathway that facilitates mitotic slippage.
  JOURNAL   J Cell Biol. 2016 Oct 24;215(2):151-166. Epub 2016 Oct 17.
  PUBMED    27810909
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2016-10-24"; volume: "215(2)"; pages: "151-166. Epub 
            2016 Oct 17"
COMMENT     Sequence Label: pFLAG-ZYG11A
FEATURES             Location/Qualifiers
     source          1..2411
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             59..82
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcttggtac cgagctcgga tccactagtc cagtgtggtg gaattcgccg ccaccatgga
       61 ttacaaggat gacgacgata agtctggcgt tcatttcttg cacccgggcc acacgccccg
      121 gaacatcgtc cctcctgacg ctcagaagga tgccctgggc tgctgcgtgg tacaggaaga
      181 ggcatctccc tacactttgg tcaacatctg cctgaatgtc ctgattgcta acctggagaa
      241 attgtgttct gaaagacctg atggaacact gtgccttccg gagcattgga gtttccctca
      301 ggaagtagcc gagcgatttc tcagggtgat gacttggcaa ggcaagctga ctgacagaac
      361 agccagcatt ttccgaggca accaaatgaa actgaagctg gtcaatatcc aaaaagctaa
      421 aatctctaca gctgcattca taaaagcctt ctgccgtcat aagctcattg aactgaatgc
      481 tactgcagtg cacgctgacc tcccagttcc agacatcata agtggactct gcagcaatag
      541 gtggatccag caaaacctcc agtgtctcct gttagactcg acaagcatcc ctcaaaattc
      601 aagattactg ttctttagtc agctcactgg tcttcgcatt ttaagtgttt ttaatgtttg
      661 ttttcatact gaagacctgg ctaatgtttc tcagttacca agactggaaa gcttagatat
      721 ctctaatact ctagtcactg atatttctgc actgcttacc tgtaaggatc gattgaagtc
      781 tctcacaatg cactatctga aatgcctggc catgaccaaa tcacaaattc ttgcagtcat
      841 tagagaactt aaatgtctgc ttcaccttga tatttctgat cacaggcaac tcaaatcaga
      901 cctagctttt catttgctac agcagaagga tatcctgccc aatgttgtgt cattggatat
      961 ttctgggggc aattgcatca ctgatgaagc tgtagaactg tttatacgac tgcggcctgc
     1021 catgcaattt gtgggactat tggccacgga tgctggctct tctgacttct ttactacaaa
     1081 gcaaggcttg agggttgctg gaggagccag tatgagtcag atttcagaag cactgagccg
     1141 atacaggaac agatcatgtt ttgtgaagga agccctccac aggctgttca cagagacatt
     1201 ttcaatggag gtaaccatgc ctgctatttt aaagcttgtg gctataggaa tgaggaatca
     1261 cccattggat ttgcgagtgc agttcacagc cagtgcttgc gctctcaacc taacacgcca
     1321 gggcctggcc aaggggatgc ctgttcgcct gttgtcagag gtcacctgtc tacttttcaa
     1381 ggctctgaaa aatttccccc attaccagca gttacagaag aattgtcttc tctccttaac
     1441 caattccagg attcttgtgg atgttccatt tgacaggttt gatgctgcca agtttgtcat
     1501 gagatggctc tgtaagcatg aaaaccccaa gatgcaaaca atggcagtga gtgtcacctc
     1561 tattctggct ctgcagctct cacctgagca aacggcacag cttgaagagc ttttcatggc
     1621 agttaaggaa cttctagcaa tagtaaaaca aaagactact gagaatttag atgatgtcac
     1681 cttcttgttt actttgaaag cactttggaa tcttacagat gggtctccag ctgcctgcaa
     1741 gcacttcatt gaaaatcaag gattgcaaat cttcatccaa gtcttggaga ccttttcaga
     1801 gtcagcaata caaagcaaag tacttggtct tttgaacaac atagcagaag tcagagagct
     1861 ctcttccaag ctggtgaccg aagatgtgct gaagcatatc aacagtttac tctgtagcag
     1921 ggaaatggaa gtcagctatt ttgctgcagg tatcatagcc cacctgacat ctgacagaca
     1981 gctttggata tcccgtgact tccagaggcg tactcttctc caagatctgc atgcaaccat
     2041 acagaattgg ccaagttcaa gttgtaagat gacagcattg gtgacctata gatctttcaa
     2101 gacatttttc ccgctccttg gcaacttctc tcaaccagag gttcagctct gggcactatg
     2161 ggctatgtat catgtctgca gtaaaaatcc cagcaaatac tgcaaaatgt tagttgaaga
     2221 agaaggattg cagcttttgt gtgatatcca ggagcacagt gaggcaaccc ccaaagcaca
     2281 gcagattgca gcctccattc tggatgactt cagaatgcat ttcatgaatt atcagaggcc
     2341 cactctgtgt caaatgccct tctgactcga gtctagaggg cccgtttaaa cccgctgatc
     2401 agcctcgact g
//
