LOCUS       sequence-207926-        5677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 droplet properties.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5677)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS1274 TDP43 RRM-GFP W385R
FEATURES             Location/Qualifiers
     source          1..5677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..44
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     26..43
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    99..123
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      142..151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1651..1675)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    1720..1854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1744..1763)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1798..1817
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1967..1987)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1967..1985)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2006..2022)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2006..2022)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2019..2041)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2030..2046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2054..2060,2061..2078,2079..2084))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2099..2120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2237..2254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2408..2996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2488..2507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3167..3958,3959..4027))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3790..3809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4028..4132)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4158..4613)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4295..4316)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4507..4526
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4684..5668
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 gcctggggac gtcggagcaa gcttgattta ggtgacacta tagaatacaa gctacttgtt
       61 ctttttgcag gatcccatcg attcgaattc aaggatcaac aagtttgtac aaaaaagcag
      121 gctggcgccg gtaccaccgg tgccaccatg ggatccatgt ctgaatatat tcgggtaacc
      181 gaagatgaga acgatgagcc cattgaaata ccatcggaag acgatgggac ggtgctgctc
      241 tccacggtta cagcccagtt tccaggggcg tgtgggcttc gctacaggaa tccagtgtct
      301 cagtgtatga gaggtgtccg gctggtagaa ggaattctgc atgccccaga tgctggctgg
      361 ggaaatctgg tgtatgttgt caactatcca aaagataaca aaagaaaaat ggatgagaca
      421 gatgcttcat cagcagtgaa agtgaaaaga gcagtccaga aaacatccag taaaggagag
      481 gaactattta caggtactgt gcctatcaaa gtcgagctgg acggcgacgt aaacggccac
      541 aagttcagcg tgcgtggcga gggcgagggc gatgccaccg agggcaagct gaccctgaag
      601 ttcatctgca ccaccggcaa gctgccggtg ccgtggccga ccctcgtgac caccctgacc
      661 tacggcgtgc agtgcttcag ccgctacccg gaccacatga agcgtcacga cttcttcaag
      721 tccgccatgc cggaaggcta cgtccaggag cgcaccatcg agttcaagga cgacggcacc
      781 tacaagaccc gcgcggaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      841 aagggcaacg acttcaagga ggacggcaac atcctgggtc acaagctgga gtacaaccac
      901 aacagccaca acgtccgtat cgaagccgac aagcagaaga acggcatcaa ggctaacttc
      961 aagatccgcc acaacgttga ggacggcagc cagcaggaag ccgaccacaa acagcagaac
     1021 accccgatcg gcgacggccc ggtgcgcctg ccggacaacc actatctgag cacccagacc
     1081 acgctgagca aagacccgaa cgagaagcgc gatcacatgg tcctgaagga gttcgtgacg
     1141 gcagccggta tcactaaagg tgaagatgaa agtggatcca atgccgaacc taagcacaat
     1201 agcaatagac agttagaaag aagtggaaga tttggtggta atccaggtgg ctttgggaat
     1261 cagggtggat ttggtaatag cagagggggt ggagctggtt tgggaaacaa tcaaggtagt
     1321 aatatgggtg gtgggatgaa ctttggtgcg ttcagcatta atccagccat gatggctgcc
     1381 gcccaggcag cactacagag cagttggggt atgatgggca tgttagccag ccagcagaac
     1441 cagtcaggcc catcgggtaa taaccaaaac caaggcaaca tgcagaggga gccaaaccag
     1501 gccttcggtt ctggaaataa ctcttatagt ggctctaatt ctggtgcagc aattggtagg
     1561 ggatcagcat ccaatgcagg gtcgggcagt ggttttaatg gaggctttgg ctcaagcatg
     1621 gattctaagt cttctggctg gggaatgtag acccagcttt cttgtacaaa gtggttgatc
     1681 ctctcgagcc tctagaacta tagtgagtcg tattacgtag atccagacat gataagatac
     1741 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     1801 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     1861 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaattc
     1921 gcggccgcgg cgccaatgca ttgggcccgg tacccagctt ttgttccctt tagtgagggt
     1981 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     2041 tcacaattcc acacaacata cgagccggga gcataaagtg taaagcctgg ggtgcctaat
     2101 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     2161 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     2221 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2281 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2341 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2401 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2461 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2521 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2581 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2641 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2701 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2761 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2821 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     2881 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2941 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3001 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3061 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3121 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3181 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3241 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3301 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3361 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3421 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3481 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     3541 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3601 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     3661 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3721 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3781 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3841 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3901 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3961 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4021 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4081 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4141 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     4201 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     4261 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     4321 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     4381 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     4441 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     4501 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     4561 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     4621 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     4681 ccagtcgacc gccaattcaa tatggcgtat atggactcat gccaattcaa tatggtggat
     4741 ctggacctgt gccaattcaa tatggcgtat atggactcgt gccaattcaa tatggtggat
     4801 ctggacccca gccaattcaa tatggcggac ttggcaccat gccaattcaa tatggcggac
     4861 ctggcactgt gccaactggg gaggggtcta cttggcacgg tgccaagttt gaggaggggt
     4921 cttggccctg tgccaagtcc gccatattga attggcatgg tgccaataat ggcggccata
     4981 ttggctatat gccaggatca atatataggc aatatccaat atggccctat gccaatatgg
     5041 ctattggcca ggttcaatac tatgtattgg ccctatgcca tatagtattc catatatggg
     5101 ttttcctatt gacgtagata gcccctccca atgggcggtc ccatatacca tatatggggc
     5161 ttcctaatac cgcccatagc cactccccca ttgacgtcaa tggtctctat atatggtctt
     5221 tcctattgac gtcatatggg cggtcctatt gacgtatatg gcgcctcccc cattgacgtc
     5281 aattacggta aatggcccgc ctggctcaat gcccattgac gtcaatagga ccacccacca
     5341 ttgacgtcaa tgggatggct cattgcccat tcatatccgt tctcacgccc cctattgacg
     5401 tcaatgacgg taaatggccc acttggcagt acatcaatat ctattaatag taacttggca
     5461 agtacattac tattggaagt acgccagggt acattggcag tactcccatt gacgtcaatg
     5521 gcggtaaatg gcccgcgatg gctgccaagt acatccccat tgacgtcaat ggggaggggc
     5581 aatgacgcaa atgggcgttc cattgacgta aatgggcggt aggcgtgcct aatgggaggt
     5641 ctatataagc aatgctcgtt tagggaaccg ccattct
//
