LOCUS       sequence-207930-        5682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 droplet properties.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5682)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS999 TDP43 RRM-GFP A382P
FEATURES             Location/Qualifiers
     source          1..5682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        23..41
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     23..40
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    96..120
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      139..148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1653..1677)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    1722..1856
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1746..1765)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1800..1819
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1969..1989)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1969..1987)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2008..2024)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2008..2024)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2021..2043)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2032..2048
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2056..2062,2063..2080,2081..2086))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2101..2122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2239..2256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2410..2998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2490..2509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3169..3960,3961..4029))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3792..3811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4030..4134)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4160..4615)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4297..4318)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4509..4528
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4686..5670
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 tggggacgtc ggagcaagct tgatttaggt gacactatag aatacaagct acttgttctt
       61 tttgcaggat cccatcgatt cgaattcaag gatcaacaag tttgtacaaa aaagcaggct
      121 ggcgccggta ccaccggtgc caccatggga tccatgtctg aatatattcg ggtaaccgaa
      181 gatgagaacg atgagcccat tgaaatacca tcggaagacg atgggacggt gctgctctcc
      241 acggttacag cccagtttcc aggggcgtgt gggcttcgct acaggaatcc agtgtctcag
      301 tgtatgagag gtgtccggct ggtagaagga attctgcatg ccccagatgc tggctgggga
      361 aatctggtgt atgttgtcaa ctatccaaaa gataacaaaa gaaaaatgga tgagacagat
      421 gcttcatcag cagtgaaagt gaaaagagca gtccagaaaa catccagtaa aggagaggaa
      481 ctatttacag gtactgtgcc tatcaaagtc gagctggacg gcgacgtaaa cggccacaag
      541 ttcagcgtgc gtggcgaggg cgagggcgat gccaccgagg gcaagctgac cctgaagttc
      601 atctgcacca ccggcaagct gccggtgccg tggccgaccc tcgtgaccac cctgacctac
      661 ggcgtgcagt gcttcagccg ctacccggac cacatgaagc gtcacgactt cttcaagtcc
      721 gccatgccgg aaggctacgt ccaggagcgc accatcgagt tcaaggacga cggcacctac
      781 aagacccgcg cggaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      841 ggcaacgact tcaaggagga cggcaacatc ctgggtcaca agctggagta caaccacaac
      901 agccacaacg tccgtatcga agccgacaag cagaagaacg gcatcaaggc taacttcaag
      961 atccgccaca acgttgagga cggcagccag caggaagccg accacaaaca gcagaacacc
     1021 ccgatcggcg acggcccggt gcgcctgccg gacaaccact atctgagcac ccagaccacg
     1081 ctgagcaaag acccgaacga gaagcgcgat cacatggtcc tgaaggagtt cgtgacggca
     1141 gccggtatca ctaaaggtga agatgaaagt ggatccaatg ccgaacctaa gcacaatagc
     1201 aatagacagt tagaaagaag tggaagattt ggtggtaatc caggtggctt tgggaatcag
     1261 ggtggatttg gtaatagcag agggggtgga gctggtttgg gaaacaatca aggtagtaat
     1321 atgggtggtg ggatgaactt tggtgcgttc agcattaatc cagccatgat ggctgccgcc
     1381 caggcagcac tacagagcag ttggggtatg atgggcatgt tagccagcca gcagaaccag
     1441 tcaggcccat cgggtaataa ccaaaaccaa ggcaacatgc agagggagcc aaaccaggcc
     1501 ttcggttctg gaaataactc ttatagtggc tctaattctg gtgcaccaat tggttgggga
     1561 tcagcatcca atgcagggtc gggcagtggt tttaatggag gctttggctc aagcatggat
     1621 tctaagtctt ctggctgggg aatgtagact agacccagct ttcttgtaca aagtggttga
     1681 tcctctcgag cctctagaac tatagtgagt cgtattacgt agatccagac atgataagat
     1741 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     1801 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     1861 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaat
     1921 tcgcggccgc ggcgccaatg cattgggccc ggtacccagc ttttgttccc tttagtgagg
     1981 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     2041 gctcacaatt ccacacaaca tacgagccgg gagcataaag tgtaaagcct ggggtgccta
     2101 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     2161 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2221 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2281 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2341 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2401 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2461 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2521 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2581 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2641 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2701 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2761 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2821 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2881 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2941 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3001 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3061 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3121 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3181 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3241 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3301 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3361 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3421 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3481 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3541 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3601 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3661 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     3721 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3781 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     3841 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     3901 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     3961 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4021 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4081 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     4141 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     4201 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     4261 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     4321 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     4381 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     4441 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     4501 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     4561 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     4621 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     4681 cgccagtcga ccgccaattc aatatggcgt atatggactc atgccaattc aatatggtgg
     4741 atctggacct gtgccaattc aatatggcgt atatggactc gtgccaattc aatatggtgg
     4801 atctggaccc cagccaattc aatatggcgg acttggcacc atgccaattc aatatggcgg
     4861 acctggcact gtgccaactg gggaggggtc tacttggcac ggtgccaagt ttgaggaggg
     4921 gtcttggccc tgtgccaagt ccgccatatt gaattggcat ggtgccaata atggcggcca
     4981 tattggctat atgccaggat caatatatag gcaatatcca atatggccct atgccaatat
     5041 ggctattggc caggttcaat actatgtatt ggccctatgc catatagtat tccatatatg
     5101 ggttttccta ttgacgtaga tagcccctcc caatgggcgg tcccatatac catatatggg
     5161 gcttcctaat accgcccata gccactcccc cattgacgtc aatggtctct atatatggtc
     5221 tttcctattg acgtcatatg ggcggtccta ttgacgtata tggcgcctcc cccattgacg
     5281 tcaattacgg taaatggccc gcctggctca atgcccattg acgtcaatag gaccacccac
     5341 cattgacgtc aatgggatgg ctcattgccc attcatatcc gttctcacgc cccctattga
     5401 cgtcaatgac ggtaaatggc ccacttggca gtacatcaat atctattaat agtaacttgg
     5461 caagtacatt actattggaa gtacgccagg gtacattggc agtactccca ttgacgtcaa
     5521 tggcggtaaa tggcccgcga tggctgccaa gtacatcccc attgacgtca atggggaggg
     5581 gcaatgacgc aaatgggcgt tccattgacg taaatgggcg gtaggcgtgc ctaatgggag
     5641 gtctatataa gcaatgctcg tttagggaac cgccattctg cc
//
