LOCUS       sequence-207934-        5677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 droplet properties.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5677)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS1249 TDP43 RRM-GFP G295C
FEATURES             Location/Qualifiers
     source          1..5677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        12..30
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     12..29
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    85..109
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      128..137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1637..1661)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    1706..1840
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1730..1749)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1784..1803
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1953..1973)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1953..1971)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1992..2008)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1992..2008)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2005..2027)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2016..2032
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2040..2046,2047..2064,2065..2070))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2085..2106
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2223..2240)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2394..2982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2474..2493)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3153..3944,3945..4013))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3776..3795
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4014..4118)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4144..4599)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4281..4302)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4493..4512
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4670..5654
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 gagcaagctt gatttaggtg acactataga atacaagcta cttgttcttt ttgcaggatc
       61 ccatcgattc gaattcaagg atcaacaagt ttgtacaaaa aagcaggctg gcgccggtac
      121 caccggtgcc accatgggat ccatgtctga atatattcgg gtaaccgaag atgagaacga
      181 tgagcccatt gaaataccat cggaagacga tgggacggtg ctgctctcca cggttacagc
      241 ccagtttcca ggggcgtgtg ggcttcgcta caggaatcca gtgtctcagt gtatgagagg
      301 tgtccggctg gtagaaggaa ttctgcatgc cccagatgct ggctggggaa atctggtgta
      361 tgttgtcaac tatccaaaag ataacaaaag aaaaatggat gagacagatg cttcatcagc
      421 agtgaaagtg aaaagagcag tccagaaaac atccagtaaa ggagaggaac tatttacagg
      481 tactgtgcct atcaaagtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgcg
      541 tggcgagggc gagggcgatg ccaccgaggg caagctgacc ctgaagttca tctgcaccac
      601 cggcaagctg ccggtgccgt ggccgaccct cgtgaccacc ctgacctacg gcgtgcagtg
      661 cttcagccgc tacccggacc acatgaagcg tcacgacttc ttcaagtccg ccatgccgga
      721 aggctacgtc caggagcgca ccatcgagtt caaggacgac ggcacctaca agacccgcgc
      781 ggaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcaacgactt
      841 caaggaggac ggcaacatcc tgggtcacaa gctggagtac aaccacaaca gccacaacgt
      901 ccgtatcgaa gccgacaagc agaagaacgg catcaaggct aacttcaaga tccgccacaa
      961 cgttgaggac ggcagccagc aggaagccga ccacaaacag cagaacaccc cgatcggcga
     1021 cggcccggtg cgcctgccgg acaaccacta tctgagcacc cagaccacgc tgagcaaaga
     1081 cccgaacgag aagcgcgatc acatggtcct gaaggagttc gtgacggcag ccggtatcac
     1141 taaaggtgaa gatgaaagtg gatccaatgc cgaacctaag cacaatagca atagacagtt
     1201 agaaagaagt ggaagatttg gtggtaatcc aggtggcttt gggaatcagg gtggatttgg
     1261 taatagcaga gggtgtggag ctggtttggg aaacaatcaa ggtagtaata tgggtggtgg
     1321 gatgaacttt ggtgcgttca gcattaatcc agccatgatg gctgccgccc aggcagcact
     1381 acagagcagt tggggtatga tgggcatgtt agccagccag cagaaccagt caggcccatc
     1441 gggtaataac caaaaccaag gcaacatgca gagggagcca aaccaggcct tcggttctgg
     1501 aaataactct tatagtggct ctaattctgg tgcagcaatt ggttggggat cagcatccaa
     1561 tgcagggtcg ggcagtggtt ttaatggagg ctttggctca agcatggatt ctaagtcttc
     1621 tggctgggga atgtagaccc agctttcttg tacaaagtgg ttgatcctct cgagcctcta
     1681 gaactatagt gagtcgtatt acgtagatcc agacatgata agatacattg atgagtttgg
     1741 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     1801 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     1861 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taattcgcgg ccgcggcgcc
     1921 aatgcattgg gcccggtacc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     1981 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     2041 aacatacgag ccgggagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     2101 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     2161 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     2221 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2281 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2341 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2401 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2461 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2521 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2581 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2641 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2701 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2761 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2821 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     2881 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     2941 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3001 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3061 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3121 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     3181 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3241 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3301 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3361 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3421 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3481 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     3541 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     3601 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     3661 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     3721 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     3781 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     3841 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     3901 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     3961 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4021 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4081 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4141 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     4201 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     4261 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     4321 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     4381 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     4441 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     4501 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     4561 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc
     4621 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag tcgaccgcca
     4681 attcaatatg gcgtatatgg actcatgcca attcaatatg gtggatctgg acctgtgcca
     4741 attcaatatg gcgtatatgg actcgtgcca attcaatatg gtggatctgg accccagcca
     4801 attcaatatg gcggacttgg caccatgcca attcaatatg gcggacctgg cactgtgcca
     4861 actggggagg ggtctacttg gcacggtgcc aagtttgagg aggggtcttg gccctgtgcc
     4921 aagtccgcca tattgaattg gcatggtgcc aataatggcg gccatattgg ctatatgcca
     4981 ggatcaatat ataggcaata tccaatatgg ccctatgcca atatggctat tggccaggtt
     5041 caatactatg tattggccct atgccatata gtattccata tatgggtttt cctattgacg
     5101 tagatagccc ctcccaatgg gcggtcccat ataccatata tggggcttcc taataccgcc
     5161 catagccact cccccattga cgtcaatggt ctctatatat ggtctttcct attgacgtca
     5221 tatgggcggt cctattgacg tatatggcgc ctcccccatt gacgtcaatt acggtaaatg
     5281 gcccgcctgg ctcaatgccc attgacgtca ataggaccac ccaccattga cgtcaatggg
     5341 atggctcatt gcccattcat atccgttctc acgcccccta ttgacgtcaa tgacggtaaa
     5401 tggcccactt ggcagtacat caatatctat taatagtaac ttggcaagta cattactatt
     5461 ggaagtacgc cagggtacat tggcagtact cccattgacg tcaatggcgg taaatggccc
     5521 gcgatggctg ccaagtacat ccccattgac gtmaatgggg rgkggsartg mcgcaaatgg
     5581 gmgytccatt gacgtaaatg ggcggtaggc gtgcctaatg ggaggtctat ataagcaatg
     5641 ctcgtttagg gaaccgccat tctgcctggg gacgtcg
//
