LOCUS       sequence-207941-        4400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid serves as a backbone for heterologous expression in the 
            model archaeal organism Methanococcus maripaludis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Lyu Z, Jain R, Smith P, Fetchko T, Yan Y, Whitman WB
  TITLE     Engineering the Autotroph Methanococcus maripaludis for Geraniol 
            Production.
  JOURNAL   ACS Synth Biol. 2016 Jul 15;5(7):577-81. doi: 
            10.1021/acssynbio.5b00267. Epub 2016 Feb 17.
  PUBMED    26886063
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00267"; journalName: "ACS Synth Biol"; date: 
            "2016-07-15- 15"; volume: "5"; issue: "7"; pages: "577-81"
COMMENT     Sequence Label: pMEV4
FEATURES             Location/Qualifiers
     source          1..4400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    219..239
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(1514..1533)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1633..1655
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1693..1711)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1779..1883
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1884..1952,1953..2744)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2102..2121)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2915..3503
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3404..3423
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttatcgagat aagaattact agatgaattc gcgcccggtt ctagacgaga taagaattac
       61 tagataattc agctgatcga tcaaaatata acataaataa cataggttta aataatttaa
      121 aggcatattt tatataaaca attgtaaaat attggcttat gaaatttgtt aaaatttagc
      181 taataagcta ttgatattat aattttatag ataactaata ctagtagcgg ccgctgcaga
      241 taaaaaacgc cctattcgta atatactatt caaaactaaa tattcattga aatatttggg
      301 taataattaa tattaatcta atatttagat attgagatat aataataaca tttttttaaa
      361 aaatcatcta ttttcatctt aattttatat attaaagccc attttttgag tattcaaatt
      421 caaattattg tgttattaac atcttatata taaacttttc tatttaatgt taatgaaaaa
      481 gtgaatatat atacatagag taatgttatg atgtatatat caaaaaaaac tagaggagac
      541 gaccttccat gacagaatat aaaccaacag ttagattagc aacaagagat gacgtaccta
      601 gggcagtaag aactcttgca gcagcttttg cagattatcc agcaacaaga cacacagttg
      661 atccagatag acacattgaa agagttacag aattacaaga attattttta acaagagttg
      721 gtcttgatat aggtaaagta tgggttgcag atgatggagc agcagttgca gtttggacaa
      781 caccagaatc agttgaagct ggtgctgttt ttgcagaaat tggaccaaga atggctgaat
      841 tatcaggatc aagattagca gctcagcaac agatggaagg attacttgca ccacacagac
      901 caaaagaacc agcatggttt ttagcaacag ttggagtttc accagatcac cagggaaaag
      961 gtttaggttc tgctgttgta cttcctggtg ttgaagcagc tgaaagagca ggagttccag
     1021 catttttaga aacatcagca ccaagaaatc ttccatttta tgaaagactt ggatttacag
     1081 ttacagcaga tgttgaagtt ccagaaggac caagaacatg gtgtatgaca agaaaaccag
     1141 gagcataacc atggctccga ccgaagccac ccggggcggc cccgccgacc ccgcacccgc
     1201 ccccgaggcc caccgcgggg gacacaccga acacgccgac cctgctgaac acgcggcgca
     1261 gttcggtgcc caggagcgga tcgggaatta attcgaagct gctggtgaaa gagaccctat
     1321 cttacctgct aaaatctaag ttaattacta atttattatt aatttattat tagattgggt
     1381 aaaatagtaa aagaaaacta aaggaaacct aatatggttt ccttttttta tatattttta
     1441 attcactggg gggatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1501 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     1561 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     1621 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     1681 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     1741 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1801 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1861 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     1921 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     1981 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     2041 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     2101 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     2161 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2221 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2281 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     2341 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2401 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     2461 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2521 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     2581 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     2641 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     2701 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     2761 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     2821 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2881 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2941 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3001 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3061 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3121 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3181 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3241 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3301 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3361 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3421 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3481 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3541 tttgctggcc ttttgctcac atggtaccca ttatttagtt tttttttatc accacaacgt
     3601 ataaaccccc tttcagaaga aaacagagaa tgaaaacaga ttttaattta tatatcgtat
     3661 ctataattta gttttttttt attgcagttt tagaagtgcg tttgtcctgg agcgaaatac
     3721 acgatatcgt gtagtctcct tcagatgaaa ataatatttt taattttcag aaatattact
     3781 ggaaaaatac ctgaaaaata tttgaaaaaa aaacatacac ccagcgggga aaaatcgaac
     3841 attttgaaaa agtctattta tagggtttaa acagggccaa gacctgcggg atatacccca
     3901 aaaatgttcg ataaaaaaat cgaacatttt tcgaacattt tttataatgt tcgatgtgtg
     3961 cgagtatgtt cgataaaaaa atcgaacatt ttgaaagaca atttgaagag atataaaaaa
     4021 aataatctat tataatatag atatataata tgttcgatat gttcgatatt tttttatata
     4081 ttatattata tttaaccttg tattgattat tgaataatat ttattattac tttttttgat
     4141 ttcttgtaaa aaatcgaaca tattttacat attgtaaaaa ataaaaaaga tttaaaaaaa
     4201 gtgtccgaaa aagttaaaca aacattatta ttaaaatgtt agtttaatat gtatcttgat
     4261 atgctcttct ttctttcatg cttaaacttg tataactgta tgcttttttc atttccttta
     4321 ttttgtcctt tcctttcttt gttatctggt aatatccttc cttctttcct gcatacttca
     4381 aaaatccaag gttttttaag
//
