LOCUS       sequence-207959-        5677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 droplet properties.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5677)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS1278 TDP43 RRM-GFP N345R
FEATURES             Location/Qualifiers
     source          1..5677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..40
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     22..39
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      138..147
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1647..1671)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    1716..1850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1740..1759)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1794..1813
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1963..1983)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1963..1981)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2002..2018)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2002..2018)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2015..2037)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2026..2042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2050..2056,2057..2074,2075..2080))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2095..2116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2233..2250)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2404..2992)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2484..2503)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3163..3954,3955..4023))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3786..3805
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4024..4128)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4154..4609)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4291..4312)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4503..4522
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4680..5664
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 ggggacgtcg gagcaagctt gatttaggtg acactataga atacaagcta cttgttcttt
       61 ttgcaggatc ccatcgattc gaattcaagg atcaacaagt ttgtacaaaa aagcaggctg
      121 gcgccggtac caccggtgcc accatgggat ccatgtctga atatattcgg gtaaccgaag
      181 atgagaacga tgagcccatt gaaataccat cggaagacga tgggacggtg ctgctctcca
      241 cggttacagc ccagtttcca ggggcgtgtg ggcttcgcta caggaatcca gtgtctcagt
      301 gtatgagagg tgtccggctg gtagaaggaa ttctgcatgc cccagatgct ggctggggaa
      361 atctggtgta tgttgtcaac tatccaaaag ataacaaaag aaaaatggat gagacagatg
      421 cttcatcagc agtgaaagtg aaaagagcag tccagaaaac atccagtaaa ggagaggaac
      481 tatttacagg tactgtgcct atcaaagtcg agctggacgg cgacgtaaac ggccacaagt
      541 tcagcgtgcg tggcgagggc gagggcgatg ccaccgaggg caagctgacc ctgaagttca
      601 tctgcaccac cggcaagctg ccggtgccgt ggccgaccct cgtgaccacc ctgacctacg
      661 gcgtgcagtg cttcagccgc tacccggacc acatgaagcg tcacgacttc ttcaagtccg
      721 ccatgccgga aggctacgtc caggagcgca ccatcgagtt caaggacgac ggcacctaca
      781 agacccgcgc ggaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      841 gcaacgactt caaggaggac ggcaacatcc tgggtcacaa gctggagtac aaccacaaca
      901 gccacaacgt ccgtatcgaa gccgacaagc agaagaacgg catcaaggct aacttcaaga
      961 tccgccacaa cgttgaggac ggcagccagc aggaagccga ccacaaacag cagaacaccc
     1021 cgatcggcga cggcccggtg cgcctgccgg acaaccacta tctgagcacc cagaccacgc
     1081 tgagcaaaga cccgaacgag aagcgcgatc acatggtcct gaaggagttc gtgacggcag
     1141 ccggtatcac taaaggtgaa gatgaaagtg gatccaatgc cgaacctaag cacaatagca
     1201 atagacagtt agaaagaagt ggaagatttg gtggtaatcc aggtggcttt gggaatcagg
     1261 gtggatttgg taatagcaga gggggtggag ctggtttggg aaacaatcaa ggtagtaata
     1321 tgggtggtgg gatgaacttt ggtgcgttca gcattaatcc agccatgatg gctgccgccc
     1381 aggcagcact acagagcagt tggggtatga tgggcatgtt agccagccag cagcgtcagt
     1441 caggcccatc gggtaataac caaaaccaag gcaacatgca gagggagcca aaccaggcct
     1501 tcggttctgg aaataactct tatagtggct ctaattctgg tgcagcaatt ggttggggat
     1561 cagcatccaa tgcagggtcg ggcagtggtt ttaatggagg ctttggctca agcatggatt
     1621 ctaagtcttc tggctgggga atgtagaccc agctttcttg tacaaagtgg ttgatcctct
     1681 cgagcctcta gaactatagt gagtcgtatt acgtagatcc agacatgata agatacattg
     1741 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     1801 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     1861 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taattcgcgg
     1921 ccgcggcgcc aatgcattgg gcccggtacc cagcttttgt tccctttagt gagggttaat
     1981 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     2041 aattccacac aacatacgag ccgggagcat aaagtgtaaa gcctggggtg cctaatgagt
     2101 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     2161 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2221 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2281 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2341 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2401 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2461 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2521 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2581 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2641 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2701 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2761 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2821 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2881 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2941 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3001 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3061 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3121 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3181 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3241 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3301 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3361 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3421 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3481 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3541 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3601 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3661 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3721 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3781 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3841 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3901 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3961 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4021 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4081 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4141 aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     4201 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     4261 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     4321 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     4381 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     4441 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     4501 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     4561 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
     4621 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     4681 tcgaccgcca attcaatatg gcgtatatgg actcatgcca attcaatatg gtggatctgg
     4741 acctgtgcca attcaatatg gcgtatatgg actcgtgcca attcaatatg gtggatctgg
     4801 accccagcca attcaatatg gcggacttgg caccatgcca attcaatatg gcggacctgg
     4861 cactgtgcca actggggagg ggtctacttg gcacggtgcc aagtttgagg aggggtcttg
     4921 gccctgtgcc aagtccgcca tattgaattg gcatggtgcc aataatggcg gccatattgg
     4981 ctatatgcca ggatcaatat ataggcaata tccaatatgg ccctatgcca atatggctat
     5041 tggccaggtt caatactatg tattggccct atgccatata gtattccata tatgggtttt
     5101 cctattgacg tagatagccc ctcccaatgg gcggtcccat ataccatata tggggcttcc
     5161 taataccgcc catagccact cccccattga cgtcaatggt ctctatatat ggtctttcct
     5221 attgacgtca tatgggcggt cctattgacg tatatggcgc ctcccccatt gacgtcaatt
     5281 acggtaaatg gcccgcctgg ctcaatgccc attgacgtca ataggaccac ccaccattga
     5341 cgtcaatggg atggctcatt gcccattcat atccgttctc acgcccccta ttgacgtcaa
     5401 tgacggtaaa tggcccactt ggcagtacat caatatctat taatagtaac ttggcaagta
     5461 cattactatt ggaagtacgc cagggtacat tggcagtact cccattgacg tcaatggcgg
     5521 taaatggccc gcgatggctg ccaagtacat ccccattgac gtcaatgggg aggggcaatg
     5581 acgcaaatgg gcgttccatt gacgtaaatg ggcggtaggc gtgcctaatg ggaggtctat
     5641 ataagcaatg ctcgtttagg gaaccgccat tctgcct
//
