LOCUS       sequence-207966-        1953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expresion of EYFP-tagged Talin 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1953)
  TITLE     expression of Talin 1 and Talin 2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EYFP-Talin1-FERM
FEATURES             Location/Qualifiers
     source          1..1953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcg
      721 aattcgggcc ccccctgccc cgccgccgcc catatggttg cgctttcgct gaagattagc
      781 attgggaatg tggtgaagac gatgcaattt gagccatcta ccatggtgta cgacgcctgc
      841 cgcatgattc gtgagcggat cccagaggcc ctggctggcc ctcccaacga ctttgggctc
      901 tttctgtcag atgatgaccc caaaaaaggc atctggctag aggctgggaa agctttggac
      961 tactacatgc tccgaaatgg ggacaccatg gagtacagaa aaaagcagag acccctgaag
     1021 atccggatgt tagacggaac ggtgaagact atcatggtgg atgactccaa gaccgtcaca
     1081 gacatgctca tgaccatttg tgcccgaatt ggtatcacca accatgatga gtattcactg
     1141 gttcgagagc tgatggaaga aaagaaagat gaggggacag ggaccctgag aaaagacaag
     1201 accctactgc gagatgaaaa gaagatggag aagctaaagc agaagttgca cacagacgat
     1261 gagttgaact ggctggacca tgggcggaca ctgagggaac agggagtgga agagcatgag
     1321 acgttgctgc tgcggagaaa gttcttctac tcagaccaga atgtggattc ccgagaccct
     1381 gtacagctga accttctcta tgtgcaggca cgagatgaca tcctgaatgg ctcccatcct
     1441 gtctcctttg acaaggcctg tgaatttgca ggcttccagt gccagatcca gtttggacct
     1501 cacaatgaac agaagcacaa ggctggcttc cttgacctga aggacttcct gcccaaggag
     1561 tatgtgaagc agaagggaga gcgtaagatc tttcaggcac acaagaattg tgggcagatg
     1621 agtgaaattg aggccaaggt acgctacgtg aagctggccc gttccctcaa gacttacggt
     1681 gtctccttct tcctagtcaa ggaaaagatg aaggggaaga ataaactggt gcccaggctg
     1741 ttgggcatca ctaaggagtg tgtgatgcgt gtggatgaga agaccaagga ggtgattcag
     1801 gaatggagcc tcaccaacat caaacgctgg gctgcctctc ccaagagctt tactctggac
     1861 tttggagact atcaggatgg ctactactca gtacagacga cagaaggcga gcagatcgca
     1921 cagctcattg ctggctacat agatatcatc ctt
//
