LOCUS       sequence-207969-        5677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 droplet properties.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Schmidt HB, Barreau A, Rohatgi R
  TITLE     Phase separation-deficient TDP43 remains functional in splicing.
  JOURNAL   Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2.
  PUBMED    31653829
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-25"; volume: "10(1):4890. doi"; pages: " 
            10.1038/s41467-019-12740-2"
COMMENT     Sequence Label: pHBS1279 TDP43 RRM-GFP G348V
FEATURES             Location/Qualifiers
     source          1..5677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    110..134
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      153..162
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1662..1686)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    1731..1865
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1755..1774)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1809..1828
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1978..1998)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1978..1996)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2017..2033)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2017..2033)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2030..2052)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2041..2057
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2065..2071,2072..2089,2090..2095))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2131
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2248..2265)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2419..3007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2499..2518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3178..3969,3970..4038))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3801..3820
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4039..4143)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4169..4624)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4306..4327)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4518..4537
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(4695..5677,1..2)
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggatcaa caagtttgta
      121 caaaaaagca ggctggcgcc ggtaccaccg gtgccaccat gggatccatg tctgaatata
      181 ttcgggtaac cgaagatgag aacgatgagc ccattgaaat accatcggaa gacgatggga
      241 cggtgctgct ctccacggtt acagcccagt ttccaggggc gtgtgggctt cgctacagga
      301 atccagtgtc tcagtgtatg agaggtgtcc ggctggtaga aggaattctg catgccccag
      361 atgctggctg gggaaatctg gtgtatgttg tcaactatcc aaaagataac aaaagaaaaa
      421 tggatgagac agatgcttca tcagcagtga aagtgaaaag agcagtccag aaaacatcca
      481 gtaaaggaga ggaactattt acaggtactg tgcctatcaa agtcgagctg gacggcgacg
      541 taaacggcca caagttcagc gtgcgtggcg agggcgaggg cgatgccacc gagggcaagc
      601 tgaccctgaa gttcatctgc accaccggca agctgccggt gccgtggccg accctcgtga
      661 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc ggaccacatg aagcgtcacg
      721 acttcttcaa gtccgccatg ccggaaggct acgtccagga gcgcaccatc gagttcaagg
      781 acgacggcac ctacaagacc cgcgcggagg tgaagttcga gggcgacacc ctggtgaacc
      841 gcatcgagct gaagggcaac gacttcaagg aggacggcaa catcctgggt cacaagctgg
      901 agtacaacca caacagccac aacgtccgta tcgaagccga caagcagaag aacggcatca
      961 aggctaactt caagatccgc cacaacgttg aggacggcag ccagcaggaa gccgaccaca
     1021 aacagcagaa caccccgatc ggcgacggcc cggtgcgcct gccggacaac cactatctga
     1081 gcacccagac cacgctgagc aaagacccga acgagaagcg cgatcacatg gtcctgaagg
     1141 agttcgtgac ggcagccggt atcactaaag gtgaagatga aagtggatcc aatgccgaac
     1201 ctaagcacaa tagcaataga cagttagaaa gaagtggaag atttggtggt aatccaggtg
     1261 gctttgggaa tcagggtgga tttggtaata gcagaggggg tggagctggt ttgggaaaca
     1321 atcaaggtag taatatgggt ggtgggatga actttggtgc gttcagcatt aatccagcca
     1381 tgatggctgc cgcccaggca gcactacaga gcagttgggg tatgatgggc atgttagcca
     1441 gccagcagaa ccagtcagtc ccatcgggta ataaccaaaa ccaaggcaac atgcagaggg
     1501 agccaaacca ggccttcggt tctggaaata actcttatag tggctctaat tctggtgcag
     1561 caattggttg gggatcagca tccaatgcag ggtcgggcag tggttttaat ggaggctttg
     1621 gctcaagcat ggattctaag tcttctggct ggggaatgta gacccagctt tcttgtacaa
     1681 agtggttgat cctctcgagc ctctagaact atagtgagtc gtattacgta gatccagaca
     1741 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     1801 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     1861 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     1921 ttttttaatt cgcggccgcg gcgccaatgc attgggcccg gtacccagct tttgttccct
     1981 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     2041 ttgttatccg ctcacaattc cacacaacat acgagccggg agcataaagt gtaaagcctg
     2101 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     2161 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     2221 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2281 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2341 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2401 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2461 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2521 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2581 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2641 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2701 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2761 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2821 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2881 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2941 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3001 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3061 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3121 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3181 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3241 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3301 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3361 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3421 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3481 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3541 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3601 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3661 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3721 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3781 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3841 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3901 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3961 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4021 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4081 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4141 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     4201 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     4261 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     4321 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     4381 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     4441 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     4501 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     4561 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     4621 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     4681 tcgctattac gccagtcgac cgccaattca atatggcgta tatggactca tgccaattca
     4741 atatggtgga tctggacctg tgccaattca atatggcgta tatggactcg tgccaattca
     4801 atatggtgga tctggacccc agccaattca atatggcgga cttggcacca tgccaattca
     4861 atatggcgga cctggcactg tgccaactgg ggaggggtct acttggcacg gtgccaagtt
     4921 tgaggagggg tcttggccct gtgccaagtc cgccatattg aattggcatg gtgccaataa
     4981 tggcggccat attggctata tgccaggatc aatatatagg caatatccaa tatggcccta
     5041 tgccaatatg gctattggcc aggttcaata ctatgtattg gccctatgcc atatagtatt
     5101 ccatatatgg gttttcctat tgacgtagat agcccctccc aatgggcggt cccatatacc
     5161 atatatgggg cttcctaata ccgcccatag ccactccccc attgacgtca atggtctcta
     5221 tatatggtct ttcctattga cgtcatatgg gcggtcctat tgacgtatat ggcgcctccc
     5281 ccattgacgt caattacggt aaatggcccg cctggctcaa tgcccattga cgtcaatagg
     5341 accacccacc attgacgtca atgggatggc tcattgccca ttcatatccg ttctcacgcc
     5401 ccctattgac gtcaatgacg gtaaatggcc cacttggcag tacatcaata tctattaata
     5461 gtaacttggc aagtacatta ctattggaag tacgccaggg tacattggca gtactcccat
     5521 tgacgtcaat ggcggtaaat ggcccgcgat ggctgccaag tacatcccca ttgacgtcaa
     5581 tggggagggg caatgacgca aatgggcgtt ccattgacgt aaatgggcgg taggcgtgcc
     5641 taatgggagg tctatataag caatgctcgt ttaggga
//
