LOCUS       sequence-207975-        9259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Controller plasmid [ca. 558.5 relative RBS strength at PLas81*].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9259)
  AUTHORS   Boehm CR, Grant PK, Haseloff J
  TITLE     Programmed hierarchical patterning of bacterial populations.
  JOURNAL   Nat Commun. 2018 Feb 22;9(1):776. doi: 10.1038/s41467-018-03069-3.
  PUBMED    29472537
REFERENCE   2  (bases 1 to 9259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-02-22"; volume: "9(1):776. doi"; pages: " 
            10.1038/s41467-018-03069-3"
COMMENT     Sequence Label: pCRB DRT7VTPLux*500
FEATURES             Location/Qualifiers
     source          1..9259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    26..44
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      852..923
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      940..966
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    975..991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    998..1014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      1806..1877
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1893..1920
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             2574..2585
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2592..2594,2595..2597,2598..3311)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      3331..3402
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3418..3445
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3605..4420)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3605..4420)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4328..4347
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(4635..4662)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(4678..4749)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(join(4769..5482,5483..5485,5486..5488))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     RBS             5495..5506
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     rep_origin      complement(8157..8702)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8237..8256)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9170..9188
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(9192..9235)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    9238..9259
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatacta
       61 gagtcacaca ggactactag atgaaaaaca tcaacgcgga cgacacctac cgtatcatta
      121 acaaaatcaa agcgtgtcgt tctaacaacg atattaacca atgcctgtct gatatgacca
      181 aaatggttca ctgtgaatat tatctgctgg ccatcattta tccgcacagc atggtaaaat
      241 ccgatatttc tattctggat aactacccaa aaaaatggcg tcagtactat gatgacgcca
      301 acctgatcaa gtacgatccg atcgtagatt actctaactc taaccattcc ccgattaact
      361 ggaatatctt tgagaacaac gcggttaaca aaaagtcccc aaacgttatc aaagaagcaa
      421 aaaccagcgg tctgatcacc ggtttcagct tcccgattca taccgcgaac aacggcttcg
      481 gtatgctgtc ttttgcgcac tccgaaaaag acaactacat cgattctctg ttcctgcacg
      541 cgtgcatgaa cattcctctg atcgttccgt ccctggttga taactatcgc aagattaaca
      601 ttgccaataa caaaagcaac aacgacctga ccaaacgcga gaaagaatgc ctggcatggg
      661 cgtgcgaagg taagtcctct tgggacatct ccaaaattct gggttgctct gaacgcaccg
      721 taactttcca tctgaccaac gcgcagatga aactgaacac cacgaatcgt tgtcaatcta
      781 tcagcaaagc tatcctgacc ggtgcgatcg attgcccgta tttcaaaaac taataactaa
      841 tggccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      901 gttgtttgtc ggtgaacgct ctctactaga gtcacacttg ctcaccttcg ggtgggcctt
      961 tctgcgttta taaattgtga gcggataaca attgacattg tgagcggata acaagatact
     1021 gagcacatac tagagggata ttaactaatt taacggcatc acgagtaatg gctctggtgg
     1081 atggttttct ggaactggaa cgttcttctg gtaaactgga atggagcgct atcctgcaaa
     1141 aaatggcctc tgacctgggt ttctctaaaa tcctgttcgg cctgctgccg aaagattccc
     1201 aagactacga aaacgcattc atcgtaggca attacccggc cgcgtggcgt gaacattatg
     1261 atcgtgccgg ttatgcacgt gtagatccaa ccgtttccca ttgcacccag tccgtgctgc
     1321 cgattttctg ggaaccgagc atctaccaga cccgtaaaca gcacgagttc ttcgaagagg
     1381 cgtccgcggc tggtctggtt tacggcctga cgatgccgct gcatggtgct cgtggcgaac
     1441 tgggcgctct gtctctgtcc gtagaagcag aaaaccgcgc ggaggctaac cgtttcatcg
     1501 aatctgtact gccgaccctg tggatgctga aagattatgc actgcaatcc ggcgcgggtc
     1561 tggcgttcga acacccagtt tctaaaccgg tagttctgac cagccgtgaa aaagaggtcc
     1621 tgcaatggtg cgctatcggc aaaacctcct gggagatttc tgtaatttgc aactgctctg
     1681 aagccaacgt caacttccac atgggtaaca ttcgtcgtaa attcggtgtt acttctcgcc
     1741 gcgttgcagc gattatggcc gttaacctgg gcctgattac tctgtaataa gcatgcacca
     1801 ggcatcaaat aaaacgaaag gctcagtcga aagacttggc ctttcgtttt atctgttgtt
     1861 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1921 tttatatact agagtgagct cgacctatag gatcgtatag gtttacgcaa gaaaatggtt
     1981 tgttactttc gaataaatac tagagtcaca caggactact agatgaacac gattaacatc
     2041 gctaagaacg acttctctga catcgaactg gctgctatcc cgttcaacac tctggctgac
     2101 cattacggtg agcgtttagc tcgcgaacag ttggcccttg agcatgagtc ttacgagatg
     2161 ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta aagctggtga ggttgcggat
     2221 aacgctgccg ccaagcctct catcactacc ctactcccta agatgattgc acgcatcaac
     2281 gactggtttg aggaagtgaa agctaagcgc ggcaagcgcc cgacagcctt ccagttcctg
     2341 caagaaatca agccggaagc cgtagcgtac atcaccatta agaccactct ggcttgccta
     2401 accagtgctg acaatacaac cgttcaggct gtagcaagcg caatcggtcg ggccattgag
     2461 gacgaggctc gcttcggtcg tatccgtgac cttgaagcta agcacttcaa gaaaaacgtt
     2521 gaggaacaac tcaacaagcg cgtagggcac gtctacaagt aataatacta gagaaagagg
     2581 agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     2641 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     2701 atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc
     2761 cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg
     2821 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     2881 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     2941 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     3001 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca
     3061 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     3121 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     3181 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
     3241 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     3301 tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     3361 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     3421 tcaccttcgg gtgggccttt ctgcgtttat atactagaga tgcaggcttc ctcgctcact
     3481 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3541 atctcgagtc ccgtcaagtc agcgtaatgc tctgccagtg ttacaaccaa ttaaccaatt
     3601 ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
     3661 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     3721 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
     3781 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
     3841 cgactgaatc cggtgagaat ggcaaaagct tatgcatttc cttccagact tgttcaacag
     3901 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg
     3961 attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa
     4021 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     4081 gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg
     4141 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     4201 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
     4261 gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc
     4321 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
     4381 gcggcctgga gcaagacgtt tcccgttgaa tatggctcat aacaccactt gtattactgt
     4441 ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac
     4501 atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga
     4561 aggatcagat cacctacgta attagttagt tagcccttag tgactcgaat tcgctgccgc
     4621 ttctagagta taaacgcaga aaggcccacc cgaaggtgag ccagtgtgac tctagtagag
     4681 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
     4741 ttttatttga tgcctggctc tagtattatc acttgtacag ctcgtccatg ccgagagtga
     4801 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
     4861 tgctcagctt ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
     4921 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gccgccgtcc tcgatgttgt
     4981 ggcggatctt gaagttggcc ttgatgccgt tcttctgctt gtcggcggtg atatagacgt
     5041 tgtcgctaaa gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
     5101 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
     5161 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
     5221 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcact
     5281 gcacgcccca ggacagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
     5341 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
     5401 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
     5461 tgaacagctc ctcgcccttg ctcaccatct agtatttctc ctctttctct agtattatta
     5521 cgcgaacgcg aagtccgact ctaagatgtc acggaggttc aagttacctt tagccggaag
     5581 tgctggcatt ttgtccaatt gagactcgtg caactggtca gcgaactggt cgtagaaatc
     5641 agccagtaca tcacaagact catatgtgtc aaccatagtt tcgcgcactg ctttgaacag
     5701 gttcgcagcg tcagccggaa tggtaccgaa ggagtcgtga atcagtgcaa aagattcgat
     5761 tccgtacttc tcgtgtgccc acactacagt cttacgaagg tggctaccgt cttggctgtg
     5821 tacaaagtta ggagcgatac cagactcctg tttgtgtgca tcaatctcgc tatctttgtt
     5881 ggtgttaatg gtaggctgta agcggaactg accgaggaac atcaggttca agcgcgtctg
     5941 aataggcttc ttgtattcct gccacacagg gaaaccatca ggagttaccc aatgcacagc
     6001 gcaacgcttg cgaagaatct ctccagtctt cttatctttg acctcagcag ccagcagctt
     6061 agcagcagac ttaagccagt tcattgcttc aaccgcagct accaccgtca cgctcacaga
     6121 ttcccaaatc agcttagcca tgtatccagc agcctgattc ggctgagtga acatcagacc
     6181 cttgccggaa tcaatagctg gctgaatggt atcttccagc acttgttgac ggaagccgaa
     6241 ctctttggac ccgtaagcca gcgtcatgac tgaacgctta gtcacactgc gagtaacacc
     6301 gtaagccagc cattgaccag ccagtgcctt agtgcccagc ttgactttct cagagatttc
     6361 accagtgttc tcatcggtca cggtaactac ttcgttatcg gtcccattga ttgcgtctgc
     6421 ttgtagaatc tcgttgactt tcttagcaac aatcccgtag atgtcctgaa cggtttcact
     6481 aggaagcaag ttaaccgcgc gaccacctac ctcatctcgg agcatcgcgg agaagtgctg
     6541 gatgccagag caagacccgt caaacgccag cggaagggag cagttatagc tcaggccgtg
     6601 gtgctgtacc ccagcgtact caaagcagaa cgcaaggaag cagaacggag aatcttgctc
     6661 agcccaccaa gtgttctcca gtggagactt agcgcaagcc atgatgttct cgtggttttc
     6721 ctcaatgaac ttgatgcgct cagggaacgg aaccttatcg acacccgcac agtttgcacc
     6781 gtggattttc agccagtagt aaccttcctt accgattggt ttacctttcg ccagcgtaag
     6841 cagtcctttg gtcatatcgt taccttgcgg gttgaacatt gacacagcgt aaacacgacc
     6901 gcgccagtcc atgttgtaag ggaaccagat ggccttatgg ttagcaaact tattggcttg
     6961 ctcaagcatg aactcaaggc tgatacggcg agacttgcga gccttgtcct tgcggtacac
     7021 agcagcggca gcacgtttcc acgcggtgag agcctcagga ttcatgtcga tgtcttccgg
     7081 tttcatcggg agttcttcac gctcaatcgc agggatgtcc tcgaccggac aatgcttcca
     7141 cttggtgatt acgttggcga ccgctaggac tttcttgttg attttccatg cggtgttttg
     7201 cgcaatgtta atcgctttgt acacctcagg catgtaaacg tcttcgtagc gcatcagtgc
     7261 tttcttactg tgagtacgca ccagcgccag aggacgacga ccgttagccc aatagccacc
     7321 accagtaatg ccagtccacg gcttaggagg aactacgcaa ggttggaaca tcggagagat
     7381 gccagccagc gcacctgcac gggttgcgat agcctcagcg tattcaggtg cgagttcgat
     7441 agtctcagag tcttgaccta ctacgccagc attttggcgg tgtaagctaa ccattccggt
     7501 tgactcaatg agcatctcga tgcagcgtac tcctacatga atagagtctt ccttatgcca
     7561 cgaagaccac gcctcgccac cgagtagacc cttagagagc atgtcagcct cgacaacttg
     7621 cataaatgct ttcatctagt agtcctgtgt gactctagta tttattcgaa agtaacaaac
     7681 cattttcttg cgtaaacttg tacgatccta caggtgcatc ttcccgacaa cgcagaccgt
     7741 tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc
     7801 aaccgtggct ccctcacttt ctggctggat gatggggcga ttcgggcctg gtatgagtca
     7861 gcaacacctt cttcacgagg cagacctcag cgctagcgga gtgtatactg gcttactatg
     7921 ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc
     7981 ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct
     8041 acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct
     8101 ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc
     8161 cataggctcc gcccccctga caagcatcac gaaatatgac gctcaaatca gtggtggcga
     8221 aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct
     8281 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat
     8341 tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga
     8401 accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8461 ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag
     8521 tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc
     8581 ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc
     8641 cctgcaaggc ggttttttcg ttctcagagc aagagattac gcgcagacca aaacgatctc
     8701 aagaagatca tcttattaag gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8761 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8821 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8881 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8941 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9001 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9061 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attccatggt
     9121 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     9181 cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa
     9241 ttcgcggccg cttctagag
//
