LOCUS       sequence-207976-        9276 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Controller plasmid [ca. 249.0 relative RBS strength at PLas81*].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Boehm CR, Grant PK, Haseloff J
  TITLE     Programmed hierarchical patterning of bacterial populations.
  JOURNAL   Nat Commun. 2018 Feb 22;9(1):776. doi: 10.1038/s41467-018-03069-3.
  PUBMED    29472537
REFERENCE   2  (bases 1 to 9276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-02-22"; volume: "9(1):776. doi"; pages: " 
            10.1038/s41467-018-03069-3"
COMMENT     Sequence Label: pCRB DRT7VTPLux*250
FEATURES             Location/Qualifiers
     source          1..9276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    26..44
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      852..923
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      940..966
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    975..991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    998..1014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      1806..1877
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1893..1920
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             2591..2602
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2609..2611,2612..2614,2615..3328)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      3348..3419
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3435..3462
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3622..4437)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3622..4437)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4345..4364
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(4652..4679)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(4695..4766)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(join(4786..5499,5500..5502,5503..5505))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     RBS             5512..5523
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     rep_origin      complement(8174..8719)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8254..8273)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9187..9205
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(9209..9252)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    9255..9276
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatacta
       61 gagtcacaca ggactactag atgaaaaaca tcaacgcgga cgacacctac cgtatcatta
      121 acaaaatcaa agcgtgtcgt tctaacaacg atattaacca atgcctgtct gatatgacca
      181 aaatggttca ctgtgaatat tatctgctgg ccatcattta tccgcacagc atggtaaaat
      241 ccgatatttc tattctggat aactacccaa aaaaatggcg tcagtactat gatgacgcca
      301 acctgatcaa gtacgatccg atcgtagatt actctaactc taaccattcc ccgattaact
      361 ggaatatctt tgagaacaac gcggttaaca aaaagtcccc aaacgttatc aaagaagcaa
      421 aaaccagcgg tctgatcacc ggtttcagct tcccgattca taccgcgaac aacggcttcg
      481 gtatgctgtc ttttgcgcac tccgaaaaag acaactacat cgattctctg ttcctgcacg
      541 cgtgcatgaa cattcctctg atcgttccgt ccctggttga taactatcgc aagattaaca
      601 ttgccaataa caaaagcaac aacgacctga ccaaacgcga gaaagaatgc ctggcatggg
      661 cgtgcgaagg taagtcctct tgggacatct ccaaaattct gggttgctct gaacgcaccg
      721 taactttcca tctgaccaac gcgcagatga aactgaacac cacgaatcgt tgtcaatcta
      781 tcagcaaagc tatcctgacc ggtgcgatcg attgcccgta tttcaaaaac taataactaa
      841 tggccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      901 gttgtttgtc ggtgaacgct ctctactaga gtcacacttg ctcaccttcg ggtgggcctt
      961 tctgcgttta taaattgtga gcggataaca attgacattg tgagcggata acaagatact
     1021 gagcacatac tagagggata ttaactaatt taacggcatc acgagtaatg gctctggtgg
     1081 atggttttct ggaactggaa cgttcttctg gtaaactgga atggagcgct atcctgcaaa
     1141 aaatggcctc tgacctgggt ttctctaaaa tcctgttcgg cctgctgccg aaagattccc
     1201 aagactacga aaacgcattc atcgtaggca attacccggc cgcgtggcgt gaacattatg
     1261 atcgtgccgg ttatgcacgt gtagatccaa ccgtttccca ttgcacccag tccgtgctgc
     1321 cgattttctg ggaaccgagc atctaccaga cccgtaaaca gcacgagttc ttcgaagagg
     1381 cgtccgcggc tggtctggtt tacggcctga cgatgccgct gcatggtgct cgtggcgaac
     1441 tgggcgctct gtctctgtcc gtagaagcag aaaaccgcgc ggaggctaac cgtttcatcg
     1501 aatctgtact gccgaccctg tggatgctga aagattatgc actgcaatcc ggcgcgggtc
     1561 tggcgttcga acacccagtt tctaaaccgg tagttctgac cagccgtgaa aaagaggtcc
     1621 tgcaatggtg cgctatcggc aaaacctcct gggagatttc tgtaatttgc aactgctctg
     1681 aagccaacgt caacttccac atgggtaaca ttcgtcgtaa attcggtgtt acttctcgcc
     1741 gcgttgcagc gattatggcc gttaacctgg gcctgattac tctgtaataa gcatgcacca
     1801 ggcatcaaat aaaacgaaag gctcagtcga aagacttggc ctttcgtttt atctgttgtt
     1861 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1921 tttatatact agagtgagct cgacctatag gatcgtatag gtttacgcaa gaaaatggtt
     1981 tgttactttc gaataaatac tagagggcta gttccaacga acaaaaaccc aatacaaaca
     2041 tgaacacgat taacatcgct aagaacgact tctctgacat cgaactggct gctatcccgt
     2101 tcaacactct ggctgaccat tacggtgagc gtttagctcg cgaacagttg gcccttgagc
     2161 atgagtctta cgagatgggt gaagcacgct tccgcaagat gtttgagcgt caacttaaag
     2221 ctggtgaggt tgcggataac gctgccgcca agcctctcat cactacccta ctccctaaga
     2281 tgattgcacg catcaacgac tggtttgagg aagtgaaagc taagcgcggc aagcgcccga
     2341 cagccttcca gttcctgcaa gaaatcaagc cggaagccgt agcgtacatc accattaaga
     2401 ccactctggc ttgcctaacc agtgctgaca atacaaccgt tcaggctgta gcaagcgcaa
     2461 tcggtcgggc cattgaggac gaggctcgct tcggtcgtat ccgtgacctt gaagctaagc
     2521 acttcaagaa aaacgttgag gaacaactca acaagcgcgt agggcacgtc tacaagtaat
     2581 aatactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg
     2641 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     2701 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga tctgcaccac
     2761 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg
     2821 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     2881 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     2941 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     3001 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     3061 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
     3121 catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     3181 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga
     3241 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     3301 tctcggcatg gacgagctgt acaagtaata atactagagc caggcatcaa ataaaacgaa
     3361 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     3421 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagatgc
     3481 aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     3541 ctcactcaaa ggcggtaatc tcgagtcccg tcaagtcagc gtaatgctct gccagtgtta
     3601 caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt
     3661 attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga
     3721 aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac
     3781 tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga
     3841 gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcctt
     3901 ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa
     3961 accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg
     4021 acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat
     4081 attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc
     4141 agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg
     4201 cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct
     4261 acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat
     4321 tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc
     4381 catgttggaa tttaatcgcg gcctggagca agacgtttcc cgttgaatat ggctcataac
     4441 accacttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt
     4501 atcttgtgca atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg
     4561 aacttttgct gagttgaagg atcagatcac ctacgtaatt agttagttag cccttagtga
     4621 ctcgaattcg ctgccgcttc tagagtataa acgcagaaag gcccacccga aggtgagcca
     4681 gtgtgactct agtagagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     4741 ttcgactgag cctttcgttt tatttgatgc ctggctctag tattatcact tgtacagctc
     4801 gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg
     4861 cttctcgttg gggtctttgc tcagcttgga ctgggtgctc aggtagtggt tgtcgggcag
     4921 cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgcc
     4981 gccgtcctcg atgttgtggc ggatcttgaa gttggccttg atgccgttct tctgcttgtc
     5041 ggcggtgata tagacgttgt cgctaaagta gttgtactcc agcttgtgcc ccaggatgtt
     5101 gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc
     5161 gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc
     5221 ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg
     5281 gtagcgggcg aagcactgca cgccccagga cagggtggtc acgagggtgg gccagggcac
     5341 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
     5401 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
     5461 gatgggcacc accccggtga acagctcctc gcccttgctc accatctagt atttctcctc
     5521 tttctctagt attattacgc gaacgcgaag tccgactcta agatgtcacg gaggttcaag
     5581 ttacctttag ccggaagtgc tggcattttg tccaattgag actcgtgcaa ctggtcagcg
     5641 aactggtcgt agaaatcagc cagtacatca caagactcat atgtgtcaac catagtttcg
     5701 cgcactgctt tgaacaggtt cgcagcgtca gccggaatgg taccgaagga gtcgtgaatc
     5761 agtgcaaaag attcgattcc gtacttctcg tgtgcccaca ctacagtctt acgaaggtgg
     5821 ctaccgtctt ggctgtgtac aaagttagga gcgataccag actcctgttt gtgtgcatca
     5881 atctcgctat ctttgttggt gttaatggta ggctgtaagc ggaactgacc gaggaacatc
     5941 aggttcaagc gcgtctgaat aggcttcttg tattcctgcc acacagggaa accatcagga
     6001 gttacccaat gcacagcgca acgcttgcga agaatctctc cagtcttctt atctttgacc
     6061 tcagcagcca gcagcttagc agcagactta agccagttca ttgcttcaac cgcagctacc
     6121 accgtcacgc tcacagattc ccaaatcagc ttagccatgt atccagcagc ctgattcggc
     6181 tgagtgaaca tcagaccctt gccggaatca atagctggct gaatggtatc ttccagcact
     6241 tgttgacgga agccgaactc tttggacccg taagccagcg tcatgactga acgcttagtc
     6301 acactgcgag taacaccgta agccagccat tgaccagcca gtgccttagt gcccagcttg
     6361 actttctcag agatttcacc agtgttctca tcggtcacgg taactacttc gttatcggtc
     6421 ccattgattg cgtctgcttg tagaatctcg ttgactttct tagcaacaat cccgtagatg
     6481 tcctgaacgg tttcactagg aagcaagtta accgcgcgac cacctacctc atctcggagc
     6541 atcgcggaga agtgctggat gccagagcaa gacccgtcaa acgccagcgg aagggagcag
     6601 ttatagctca ggccgtggtg ctgtacccca gcgtactcaa agcagaacgc aaggaagcag
     6661 aacggagaat cttgctcagc ccaccaagtg ttctccagtg gagacttagc gcaagccatg
     6721 atgttctcgt ggttttcctc aatgaacttg atgcgctcag ggaacggaac cttatcgaca
     6781 cccgcacagt ttgcaccgtg gattttcagc cagtagtaac cttccttacc gattggttta
     6841 cctttcgcca gcgtaagcag tcctttggtc atatcgttac cttgcgggtt gaacattgac
     6901 acagcgtaaa cacgaccgcg ccagtccatg ttgtaaggga accagatggc cttatggtta
     6961 gcaaacttat tggcttgctc aagcatgaac tcaaggctga tacggcgaga cttgcgagcc
     7021 ttgtccttgc ggtacacagc agcggcagca cgtttccacg cggtgagagc ctcaggattc
     7081 atgtcgatgt cttccggttt catcgggagt tcttcacgct caatcgcagg gatgtcctcg
     7141 accggacaat gcttccactt ggtgattacg ttggcgaccg ctaggacttt cttgttgatt
     7201 ttccatgcgg tgttttgcgc aatgttaatc gctttgtaca cctcaggcat gtaaacgtct
     7261 tcgtagcgca tcagtgcttt cttactgtga gtacgcacca gcgccagagg acgacgaccg
     7321 ttagcccaat agccaccacc agtaatgcca gtccacggct taggaggaac tacgcaaggt
     7381 tggaacatcg gagagatgcc agccagcgca cctgcacggg ttgcgatagc ctcagcgtat
     7441 tcaggtgcga gttcgatagt ctcagagtct tgacctacta cgccagcatt ttggcggtgt
     7501 aagctaacca ttccggttga ctcaatgagc atctcgatgc agcgtactcc tacatgaata
     7561 gagtcttcct tatgccacga agaccacgcc tcgccaccga gtagaccctt agagagcatg
     7621 tcagcctcga caacttgcat aaatgctttc atctagtagt cctgtgtgac tctagtattt
     7681 attcgaaagt aacaaaccat tttcttgcgt aaacttgtac gatcctacag gtgcatcttc
     7741 ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct
     7801 acaacaaagc tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc
     7861 gggcctggta tgagtcagca acaccttctt cacgaggcag acctcagcgc tagcggagtg
     7921 tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc atgtggcagg
     7981 agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata ttccgcttcc
     8041 tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa
     8101 cggggcggag atttcctgga agatgccagg aagatactta acagggaagt gagagggccg
     8161 cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa atatgacgct
     8221 caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg
     8281 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat
     8341 ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag
     8401 ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat
     8461 cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat
     8521 tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag
     8581 ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag
     8641 aaccttcgaa aaaccgccct gcaaggcggt tttttcgttc tcagagcaag agattacgcg
     8701 cagaccaaaa cgatctcaag aagatcatct tattaagggg tctgacgctc agtggaacga
     8761 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8821 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8881 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8941 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9001 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9061 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9121 ccagtctatt ccatggtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     9181 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct
     9241 aaggatgatt tctggaattc gcggccgctt ctagag
//
