LOCUS       sequence-207978-        9275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Controller plasmid [ca. 47.0 relative RBS strength at PLas81*].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9275)
  AUTHORS   Boehm CR, Grant PK, Haseloff J
  TITLE     Programmed hierarchical patterning of bacterial populations.
  JOURNAL   Nat Commun. 2018 Feb 22;9(1):776. doi: 10.1038/s41467-018-03069-3.
  PUBMED    29472537
REFERENCE   2  (bases 1 to 9275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-02-22"; volume: "9(1):776. doi"; pages: " 
            10.1038/s41467-018-03069-3"
COMMENT     Sequence Label: pCRB DRT7VTPLux*50
FEATURES             Location/Qualifiers
     source          1..9275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    26..44
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      852..923
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      940..966
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    975..991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    998..1014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      1806..1877
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1893..1920
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             2590..2601
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2608..2610,2611..2613,2614..3327)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      3347..3418
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3434..3461
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3621..4436)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3621..4436)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4344..4363
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(4651..4678)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(4694..4765)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(join(4785..5498,5499..5501,5502..5504))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     RBS             5511..5522
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     rep_origin      complement(8173..8718)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8253..8272)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9186..9204
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(9208..9251)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    9254..9275
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatacta
       61 gagtcacaca ggactactag atgaaaaaca tcaacgcgga cgacacctac cgtatcatta
      121 acaaaatcaa agcgtgtcgt tctaacaacg atattaacca atgcctgtct gatatgacca
      181 aaatggttca ctgtgaatat tatctgctgg ccatcattta tccgcacagc atggtaaaat
      241 ccgatatttc tattctggat aactacccaa aaaaatggcg tcagtactat gatgacgcca
      301 acctgatcaa gtacgatccg atcgtagatt actctaactc taaccattcc ccgattaact
      361 ggaatatctt tgagaacaac gcggttaaca aaaagtcccc aaacgttatc aaagaagcaa
      421 aaaccagcgg tctgatcacc ggtttcagct tcccgattca taccgcgaac aacggcttcg
      481 gtatgctgtc ttttgcgcac tccgaaaaag acaactacat cgattctctg ttcctgcacg
      541 cgtgcatgaa cattcctctg atcgttccgt ccctggttga taactatcgc aagattaaca
      601 ttgccaataa caaaagcaac aacgacctga ccaaacgcga gaaagaatgc ctggcatggg
      661 cgtgcgaagg taagtcctct tgggacatct ccaaaattct gggttgctct gaacgcaccg
      721 taactttcca tctgaccaac gcgcagatga aactgaacac cacgaatcgt tgtcaatcta
      781 tcagcaaagc tatcctgacc ggtgcgatcg attgcccgta tttcaaaaac taataactaa
      841 tggccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      901 gttgtttgtc ggtgaacgct ctctactaga gtcacacttg ctcaccttcg ggtgggcctt
      961 tctgcgttta taaattgtga gcggataaca attgacattg tgagcggata acaagatact
     1021 gagcacatac tagagggata ttaactaatt taacggcatc acgagtaatg gctctggtgg
     1081 atggttttct ggaactggaa cgttcttctg gtaaactgga atggagcgct atcctgcaaa
     1141 aaatggcctc tgacctgggt ttctctaaaa tcctgttcgg cctgctgccg aaagattccc
     1201 aagactacga aaacgcattc atcgtaggca attacccggc cgcgtggcgt gaacattatg
     1261 atcgtgccgg ttatgcacgt gtagatccaa ccgtttccca ttgcacccag tccgtgctgc
     1321 cgattttctg ggaaccgagc atctaccaga cccgtaaaca gcacgagttc ttcgaagagg
     1381 cgtccgcggc tggtctggtt tacggcctga cgatgccgct gcatggtgct cgtggcgaac
     1441 tgggcgctct gtctctgtcc gtagaagcag aaaaccgcgc ggaggctaac cgtttcatcg
     1501 aatctgtact gccgaccctg tggatgctga aagattatgc actgcaatcc ggcgcgggtc
     1561 tggcgttcga acacccagtt tctaaaccgg tagttctgac cagccgtgaa aaagaggtcc
     1621 tgcaatggtg cgctatcggc aaaacctcct gggagatttc tgtaatttgc aactgctctg
     1681 aagccaacgt caacttccac atgggtaaca ttcgtcgtaa attcggtgtt acttctcgcc
     1741 gcgttgcagc gattatggcc gttaacctgg gcctgattac tctgtaataa gcatgcacca
     1801 ggcatcaaat aaaacgaaag gctcagtcga aagacttggc ctttcgtttt atctgttgtt
     1861 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1921 tttatatact agagtgagct cgacctatag gatcgtatag gtttacgcaa gaaaatggtt
     1981 tgttactttc gaataaatac tagagccata cattattctc gcaagtccag aatagcgtat
     2041 gaacacgatt aacatcgcta agaacgactt ctctgacatc gaactggctg ctatcccgtt
     2101 caacactctg gctgaccatt acggtgagcg tttagctcgc gaacagttgg cccttgagca
     2161 tgagtcttac gagatgggtg aagcacgctt ccgcaagatg tttgagcgtc aacttaaagc
     2221 tggtgaggtt gcggataacg ctgccgccaa gcctctcatc actaccctac tccctaagat
     2281 gattgcacgc atcaacgact ggtttgagga agtgaaagct aagcgcggca agcgcccgac
     2341 agccttccag ttcctgcaag aaatcaagcc ggaagccgta gcgtacatca ccattaagac
     2401 cactctggct tgcctaacca gtgctgacaa tacaaccgtt caggctgtag caagcgcaat
     2461 cggtcgggcc attgaggacg aggctcgctt cggtcgtatc cgtgaccttg aagctaagca
     2521 cttcaagaaa aacgttgagg aacaactcaa caagcgcgta gggcacgtct acaagtaata
     2581 atactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg
     2641 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     2701 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc
     2761 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc
     2821 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     2881 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     2941 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     3001 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     3061 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
     3121 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     3181 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac
     3241 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     3301 ctcggcatgg acgagctgta caagtaataa tactagagcc aggcatcaaa taaaacgaaa
     3361 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     3421 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagatgca
     3481 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3541 tcactcaaag gcggtaatct cgagtcccgt caagtcagcg taatgctctg ccagtgttac
     3601 aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
     3661 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
     3721 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
     3781 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     3841 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg catttccttc
     3901 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     3961 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
     4021 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     4081 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca
     4141 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     4201 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     4261 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt
     4321 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     4381 atgttggaat ttaatcgcgg cctggagcaa gacgtttccc gttgaatatg gctcataaca
     4441 ccacttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
     4501 tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga
     4561 acttttgctg agttgaagga tcagatcacc tacgtaatta gttagttagc ccttagtgac
     4621 tcgaattcgc tgccgcttct agagtataaa cgcagaaagg cccacccgaa ggtgagccag
     4681 tgtgactcta gtagagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     4741 tcgactgagc ctttcgtttt atttgatgcc tggctctagt attatcactt gtacagctcg
     4801 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
     4861 ttctcgttgg ggtctttgct cagcttggac tgggtgctca ggtagtggtt gtcgggcagc
     4921 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgccg
     4981 ccgtcctcga tgttgtggcg gatcttgaag ttggccttga tgccgttctt ctgcttgtcg
     5041 gcggtgatat agacgttgtc gctaaagtag ttgtactcca gcttgtgccc caggatgttg
     5101 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
     5161 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
     5221 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
     5281 tagcgggcga agcactgcac gccccaggac agggtggtca cgagggtggg ccagggcacg
     5341 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
     5401 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
     5461 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatctagta tttctcctct
     5521 ttctctagta ttattacgcg aacgcgaagt ccgactctaa gatgtcacgg aggttcaagt
     5581 tacctttagc cggaagtgct ggcattttgt ccaattgaga ctcgtgcaac tggtcagcga
     5641 actggtcgta gaaatcagcc agtacatcac aagactcata tgtgtcaacc atagtttcgc
     5701 gcactgcttt gaacaggttc gcagcgtcag ccggaatggt accgaaggag tcgtgaatca
     5761 gtgcaaaaga ttcgattccg tacttctcgt gtgcccacac tacagtctta cgaaggtggc
     5821 taccgtcttg gctgtgtaca aagttaggag cgataccaga ctcctgtttg tgtgcatcaa
     5881 tctcgctatc tttgttggtg ttaatggtag gctgtaagcg gaactgaccg aggaacatca
     5941 ggttcaagcg cgtctgaata ggcttcttgt attcctgcca cacagggaaa ccatcaggag
     6001 ttacccaatg cacagcgcaa cgcttgcgaa gaatctctcc agtcttctta tctttgacct
     6061 cagcagccag cagcttagca gcagacttaa gccagttcat tgcttcaacc gcagctacca
     6121 ccgtcacgct cacagattcc caaatcagct tagccatgta tccagcagcc tgattcggct
     6181 gagtgaacat cagacccttg ccggaatcaa tagctggctg aatggtatct tccagcactt
     6241 gttgacggaa gccgaactct ttggacccgt aagccagcgt catgactgaa cgcttagtca
     6301 cactgcgagt aacaccgtaa gccagccatt gaccagccag tgccttagtg cccagcttga
     6361 ctttctcaga gatttcacca gtgttctcat cggtcacggt aactacttcg ttatcggtcc
     6421 cattgattgc gtctgcttgt agaatctcgt tgactttctt agcaacaatc ccgtagatgt
     6481 cctgaacggt ttcactagga agcaagttaa ccgcgcgacc acctacctca tctcggagca
     6541 tcgcggagaa gtgctggatg ccagagcaag acccgtcaaa cgccagcgga agggagcagt
     6601 tatagctcag gccgtggtgc tgtaccccag cgtactcaaa gcagaacgca aggaagcaga
     6661 acggagaatc ttgctcagcc caccaagtgt tctccagtgg agacttagcg caagccatga
     6721 tgttctcgtg gttttcctca atgaacttga tgcgctcagg gaacggaacc ttatcgacac
     6781 ccgcacagtt tgcaccgtgg attttcagcc agtagtaacc ttccttaccg attggtttac
     6841 ctttcgccag cgtaagcagt cctttggtca tatcgttacc ttgcgggttg aacattgaca
     6901 cagcgtaaac acgaccgcgc cagtccatgt tgtaagggaa ccagatggcc ttatggttag
     6961 caaacttatt ggcttgctca agcatgaact caaggctgat acggcgagac ttgcgagcct
     7021 tgtccttgcg gtacacagca gcggcagcac gtttccacgc ggtgagagcc tcaggattca
     7081 tgtcgatgtc ttccggtttc atcgggagtt cttcacgctc aatcgcaggg atgtcctcga
     7141 ccggacaatg cttccacttg gtgattacgt tggcgaccgc taggactttc ttgttgattt
     7201 tccatgcggt gttttgcgca atgttaatcg ctttgtacac ctcaggcatg taaacgtctt
     7261 cgtagcgcat cagtgctttc ttactgtgag tacgcaccag cgccagagga cgacgaccgt
     7321 tagcccaata gccaccacca gtaatgccag tccacggctt aggaggaact acgcaaggtt
     7381 ggaacatcgg agagatgcca gccagcgcac ctgcacgggt tgcgatagcc tcagcgtatt
     7441 caggtgcgag ttcgatagtc tcagagtctt gacctactac gccagcattt tggcggtgta
     7501 agctaaccat tccggttgac tcaatgagca tctcgatgca gcgtactcct acatgaatag
     7561 agtcttcctt atgccacgaa gaccacgcct cgccaccgag tagaccctta gagagcatgt
     7621 cagcctcgac aacttgcata aatgctttca tctagtagtc ctgtgtgact ctagtattta
     7681 ttcgaaagta acaaaccatt ttcttgcgta aacttgtacg atcctacagg tgcatcttcc
     7741 cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta
     7801 caacaaagct ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattcg
     7861 ggcctggtat gagtcagcaa caccttcttc acgaggcaga cctcagcgct agcggagtgt
     7921 atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca tgtggcagga
     7981 gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct
     8041 cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac
     8101 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc
     8161 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tatgacgctc
     8221 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg
     8281 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     8341 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
     8401 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc
     8461 gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt
     8521 gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt
     8581 tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga
     8641 accttcgaaa aaccgccctg caaggcggtt ttttcgttct cagagcaaga gattacgcgc
     8701 agaccaaaac gatctcaaga agatcatctt attaaggggt ctgacgctca gtggaacgaa
     8761 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8821 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8881 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8941 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9001 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9061 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9121 cagtctattc catggtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     9181 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta
     9241 aggatgattt ctggaattcg cggccgcttc tagag
//
